FTIR study of a nonclassical dT10*dA10-dT10 intramolecular triple helix.

abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

journal_title：Biochemistry

authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

更新日期：1992-02-11 00:00:00

abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...

journal_title：Biochemistry

authors： Moise G,Gallup NM,Alexandrova AN,Hengge AC,Johnson SJ

更新日期：2015-10-27 00:00:00

abstract：:Production of single-strand breaks (ssb) and double-strand breaks (dsb) of PM2 phage DNA by several structurally related bleomycin (BLM) analogues was studied by gel electrophoresis. BLM A2 and BLM B2 produced a comparable extent of dsb. In various experiments, BLM A2 and BLM B2, at 22-41 ng/mL, degraded 50% of the fo...

journal_title：Biochemistry

authors： Huang CH,Mirabelli CK,Jan Y,Crooke ST

更新日期：1981-01-20 00:00:00

abstract：:The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...

journal_title：Biochemistry

authors： Juszczak LJ,Desamero RZ

更新日期：2009-03-31 00:00:00

abstract：:Clostridial neurotoxins are zinc endopeptidases, and each contains one Zn(2+)/molecule. To investigate the structural/functional role of Zn(2+) in botulinum neurotoxin light chain (the enzymatic subunit of the neurotoxin), the effect of the removal of zinc on protein folding and enzyme kinetics was investigated. The a...

journal_title：Biochemistry

authors： Li L,Singh BR

更新日期：2000-08-29 00:00:00

abstract：:G proteins play an important role in transmitting hormonal signals, and fluorescence techniques would be useful to study their cellular distribution and mechanisms. To prepare active fluorescent G protein Go/Gi or beta gamma subunits were reacted with fluorescein isothiocyanate (FITC) to label the alpha (F-alpha) and ...

journal_title：Biochemistry

authors： Kwon G,Remmers AE,Datta S,Neubig RR

更新日期：1993-03-09 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00

abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...

journal_title：Biochemistry

authors： Jackson RC

更新日期：1976-12-14 00:00:00

abstract：:Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...

journal_title：Biochemistry

authors： Fromant M,Plateau P,Schmitt E,Mechulam Y,Blanquet S

更新日期：1999-04-20 00:00:00

abstract：:Deamidation of proteins is one of the most prevalent post-translational modifications found upon aging, and in age-onset diseases. Specific asparagine and glutamine residues are often selectively deamidated during this process. In the human lens, deamidation has been shown to occur in many crystallins, but it is not c...

journal_title：Biochemistry

authors： Pande A,Mokhor N,Pande J

更新日期：2015-08-11 00:00:00

abstract：:Reactive metabolites of environmental chemicals and drugs can cause site specific damage to the p53 tumor suppressor gene in a major pathway for genotoxicity. We report here a high-throughput, cell-free, 96-well plate magnetic bead-enzyme system interfaced with LC-MS/MS sequencing for bioactivating test chemicals and ...

journal_title：Biochemistry

authors： Malla S,Kadimisetty K,Jiang D,Choudhary D,Rusling JF

更新日期：2018-07-03 00:00:00

abstract：:The binding of bacteriophage T4 DNA polymerase (T4 pol) to primer-template DNA with 2-aminopurine (2AP) located at the primer terminus results in the formation of a hyperfluorescent 2AP state. Changes in this hyperfluorescent state were utilized to investigate the fractional concentration of primer-templates bound at ...

journal_title：Biochemistry

authors： Beechem JM,Otto MR,Bloom LB,Eritja R,Reha-Krantz LJ,Goodman MF

更新日期：1998-07-14 00:00:00

abstract：:Protein flexibility is thought to play key roles in numerous biological processes, including antibody affinity maturation, signal transduction, and enzyme catalysis, yet only limited information is available regarding the molecular details linking protein dynamics with function. A single point mutation at the distal s...

journal_title：Biochemistry

authors： Grossman M,Tworowski D,Dym O,Lee MH,Levy Y,Murphy G,Sagi I

更新日期：2010-07-27 00:00:00

abstract：:Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...

journal_title：Biochemistry

authors： Sheth PR,Basso A,Duca JS,Lesburg CA,Ogas P,Gray K,Nale L,Mannarino AF,Prongay AJ,Le HV

更新日期：2009-11-24 00:00:00

abstract：:The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracel...

journal_title：Biochemistry

authors： Vachon V,Paradis MJ,Marsolais M,Schwartz JL,Laprade R

更新日期：1995-11-21 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...

journal_title：Biochemistry

authors： Rim J,Oprian DD

更新日期：1995-09-19 00:00:00

abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

journal_title：Biochemistry

authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

更新日期：1999-06-22 00:00:00

abstract：:The initial steps of actomyosin subfragment 1 (acto-S1) ATPase (dissociation and binding of ATP) were studied at -15 degrees C with 40% ethylene glycol as antifreeze. The dissociation kinetics were followed by light scattering in a stopped-flow apparatus, and the binding of ATP was followed by the ATP chase method in ...

journal_title：Biochemistry

authors： Tesi C,Travers F,Barman T

更新日期：1990-02-20 00:00:00

abstract：:The role of the C-domain sites of cardiac troponin C in the modulation of the calcium signal remains unclear. In this study, we investigated the effects of hypertrophic cardiomyopathy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sites. The A8V mutation had essentially ...

journal_title：Biochemistry

authors： Swindle N,Tikunova SB

更新日期：2010-06-15 00:00:00

abstract：:A double mutant of the single-domain protein barstar having a single tryptophan (W53) was made by mutating the remaining two tryptophans (W38 and W44) into phenylalanines. W53 is buried in the core of barstar. Time-resolved fluorescence of the mutant barstar (W38FW44F) showed that W53 has a single fluorescence lifetim...

journal_title：Biochemistry

authors： Swaminathan R,Nath U,Udgaonkar JB,Periasamy N,Krishnamoorthy G

更新日期：1996-07-16 00:00:00

abstract：:The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one a...

journal_title：Biochemistry

authors： Poulos TL,Finzel BC,Howard AJ

更新日期：1986-09-09 00:00:00

abstract：:The crystallographic structure of oxygenated trHbN from Mycobacterium tuberculosis showed an extended heme distal site hydrogen-bonding network that includes Y(B10), Q(E11), and the bound O(2) (Milani, M., et al. (2001) EMBO J. 20, 3902-3909). In the present work, we analyze the effects that substitutions at the B10 a...

journal_title：Biochemistry

authors： Ouellet Y,Milani M,Couture M,Bolognesi M,Guertin M

更新日期：2006-07-25 00:00:00

abstract：:Two peptides designed for drug delivery were generated by the combination of a signal peptide with a nuclear localization sequence and are shown to facilitate the cellular internalization of small molecules which are covalently linked to these peptides. In order to understand the mechanism of internalization, the conf...

journal_title：Biochemistry

authors： Chaloin L,Vidal P,Heitz A,Van Mau N,Méry J,Divita G,Heitz F

更新日期：1997-09-16 00:00:00

abstract：:The transmission of regulatory signals between the alpha- and beta-subunits of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium has been investigated by monitoring the luminescence properties of the enzyme in the presence and in the absence of the alpha-subunit ligand DL-alpha-glycerol 3-phos...

journal_title：Biochemistry

authors： Strambini GB,Cioni P,Peracchi A,Mozzarelli A

更新日期：1992-08-25 00:00:00

abstract：:Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active m...

journal_title：Biochemistry

authors： Burger KN,Demel RA,Schmid SL,de Kruijff B

更新日期：2000-10-10 00:00:00

abstract：:13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...

journal_title：Biochemistry

authors： Hunter BK,Nicholls KM,Sanders JK

更新日期：1984-01-31 00:00:00

abstract：:The dependence of enzymatic catalysis on diffusion rates in solution was examined with regard to high specific activity carbonic anhydrase (CA II) by varying the viscosity of the reaction medium with added glycerol, sucrose, and ficoll (a copolymer of sucrose and epichlorohydrin). Responses of the Michaelis-Menten par...

journal_title：Biochemistry

authors： Pocker Y,Janjić N

更新日期：1987-05-05 00:00:00

abstract：:We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase ...

journal_title：Biochemistry

authors： Ellens H,Siegel DP,Alford D,Yeagle PL,Boni L,Lis LJ,Quinn PJ,Bentz J

更新日期：1989-05-02 00:00:00

abstract：:The pH-dependent behavior of the ferric forms of two soluble truncations of Rhizobium meliloti FixL, FixL (heme and kinase domains, functional), and FixLN (heme domain) are examined by UV-visible, resonance Raman, and electron paramagnetic resonance spectroscopy. Global analysis of UV-visible data indicates that the p...

journal_title：Biochemistry

authors： Lukat-Rodgers GS,Rexine JL,Rodgers KR

更新日期：1998-09-29 00:00:00