Abstract：
:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using bis(4-nitrophenyl) phosphate as substrate. The results suggest a ping-pong type mechanism, with participation of a covalent enzyme intermediate.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kelly SJ,Butler LGdoi
10.1021/bi00625a011subject
Has Abstractpub_date
1977-03-22 00:00:00pages
1102-4issue
6eissn
0006-2960issn
1520-4995journal_volume
16pub_type
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