当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Formation of highly ordered multimers in G-quadruplexes.

Formation of highly ordered multimers in G-quadruplexes.

Abstract：

:G-Rich DNA and RNA have a higher propensity to form G-quadruplex structures, but the presence of G-runs alone is not sufficient to prove that such sequences can form stable G-quadruplexes. While G-rich sequences are essential for G-quadruplex formation, not all G-rich sequences have the propensity to form G-quadruplex structures. In addition, monovalent metal ions, dehydrating agents, and loop sequences connecting the G-runs also play important roles in the topology of G-quadruplex folding. To date, no quantitative analysis of the CD spectra of G-quadruplexes in confrontation with the electrophoretic results has been performed. Therefore, in this study, we use information gained through the analysis of a series of well-known G-quadruplex-forming sequences to evaluate other less-studied sets of aptameric sequences. A simple and cost-effective methodology that can verify the formation of G-quadruplex motifs from oligomeric DNA sequences and a technique to determine the molecularity of these structures are also described. This methodology could be of great use in the prediction of G-quadruplex assembly, and the basic principles of our techniques can be extrapolated for any G-rich DNA sequences. This study also presents a model that can predict the multimerization of G-quadruplexes; the predictions offered by this model are shown to match the results obtained using circular dichroism.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Tóthová P,Krafčíková P,Víglaský V

doi

10.1021/bi500773c

subject

Has Abstract

pub_date

2014-11-18 00:00:00

pages

7013-27

issue

45

eissn

0006-2960

issn

1520-4995

journal_volume

53

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全