Complete amino acid sequence of the 4Fe-4S, thermostable ferredoxin from Clostridium thermoaceticum.

abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

journal_title：Biochemistry

authors： McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

更新日期：2011-09-06 00:00:00

abstract：:Cytochrome bo from Escherichia coli is a ubiquinol oxidase which is a member of the superfamily of heme-copper respiratory oxidases. This superfamily, which includes the eukaryotic cytochrome c oxidases, has in common a bimetallic center consisting of a high-spin heme component and a copper atom (CuB) which is the sit...

journal_title：Biochemistry

authors： Thomas JW,Lemieux LJ,Alben JO,Gennis RB

更新日期：1993-10-19 00:00:00

abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...

journal_title：Biochemistry

authors： Clausen H,McKibbin JM,Hakomori S

更新日期：1985-10-22 00:00:00

abstract：:Lipidic membrane systems that have been reported to be composed of sphingomyelin (SM)-cholesterol (Chol) microdomains or "rafts" by Dietrich et al. [palmitoyloleoyl-phosphatidylcholine(POPC)/SM/Chol, 1/1/1; Dietrich, C., Bagatolli, L. A., Volovyk, Z. N., Thompson, N. L., Levi, M., Jacobson, K., and Gratton, E. (2001) ...

journal_title：Biochemistry

authors： Aussenac F,Tavares M,Dufourc EJ

更新日期：2003-02-18 00:00:00

abstract：:Most nuclear receptors recognize the same consensus hexameric sequence, AGGTCA. An important question has been how the various members of this transcription factor family distinguish identity features in these closely related DNA sites. We determined structures from several crystal forms of the RevErb-DNA complex and ...

journal_title：Biochemistry

authors： Sierk ML,Zhao Q,Rastinejad F

更新日期：2001-10-30 00:00:00

abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...

journal_title：Biochemistry

authors： Lotierzo M,Raux E,Tse Sum Bui B,Goasdoue N,Libot F,Florentin D,Warren MJ,Marquet A

更新日期：2006-10-10 00:00:00

abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...

journal_title：Biochemistry

authors： Osborne RL,Zhu H,Iavarone AT,Hess CR,Klinman JP

更新日期：2012-09-25 00:00:00

abstract：:While within a human host the opportunistic pathogen Toxoplasma gondii relies heavily on glycolysis for its energy needs. Lactate dehydrogenase (LDH), the terminal enzyme in anaerobic glycolysis necessary for NAD(+) regeneration, therefore represents an attractive therapeutic target. The tachyzoite stage lactate dehyd...

journal_title：Biochemistry

authors： Kavanagh KL,Elling RA,Wilson DK

更新日期：2004-02-03 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...

journal_title：Biochemistry

authors： Gasiewicz TA,Elferink CJ,Henry EC

更新日期：1991-03-19 00:00:00

abstract：:The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...

journal_title：Biochemistry

authors： Resch CT,Winogrodzki JL,Patterson CT,Lind EJ,Quinn MJ,Dibrov P,Häse CC

更新日期：2010-03-23 00:00:00

abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...

journal_title：Biochemistry

authors： Stein RL,Melandri F,Dick L

更新日期：1996-04-02 00:00:00

abstract：:The technique of fluorescence recovery after photobleaching is used to perform an extensive study of the lateral diffusion of a phospholipid probe in the binary mixture dimyristoylphosphatidylcholine/cholesterol, above the melting temperature of the phospholipid. In the regions of the phase diagram where a single liqu...

journal_title：Biochemistry

authors： Almeida PF,Vaz WL,Thompson TE

更新日期：1992-07-28 00:00:00

abstract：:Prion diseases, also known as the transmissible spongiform encephalopathies (TSEs), are a group of fatal neurodegenerative disorders that affect humans and animals. These diseases are intimately associated with conformational conversion of the cellular prion protein, PrP(C), into an oligomeric beta-sheet-rich form, Pr...

journal_title：Biochemistry

authors： Cobb NJ,Surewicz WK

更新日期：2009-03-31 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:The acetoacetate decarboxylase-like superfamily (ADCSF) is a little-explored group of enzymes that may contain new biocatalysts. The low level of sequence identity (∼20%) between many ADCSF enzymes and the confirmed acetoacetate decarboxylases led us to investigate the degree of diversity in the reaction and substrate...

journal_title：Biochemistry

authors： Mydy LS,Hoppe RW,Hagemann TM,Schwabacher AW,Silvaggi NR

更新日期：2019-10-08 00:00:00

abstract：:Coumermycin A(1) is a member of the aminocoumarin family of antibiotics. Unlike its structural relatives, novobiocin and clorobiocin, coumermycin A(1) is a dimer built on a 3-methyl-2,4-dicarboxypyrrole scaffold and bears two decorated noviose sugar components which are the putative target binding motifs for DNA gyras...

journal_title：Biochemistry

authors： Freel Meyers CL,Oberthür M,Heide L,Kahne D,Walsh CT

更新日期：2004-11-30 00:00:00

abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...

journal_title：Biochemistry

authors： Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

更新日期：2016-03-15 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...

journal_title：Biochemistry

authors： Chu LK,Huang WY,Littledike ET,Hamilton JW,Cohn DV

更新日期：1975-08-12 00:00:00

abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

journal_title：Biochemistry

authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

更新日期：2008-08-19 00:00:00

abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...

journal_title：Biochemistry

authors： Casadio R,Stoeckenius W

更新日期：1980-07-08 00:00:00

abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...

journal_title：Biochemistry

authors： Joshi MD,Sidhu G,Nielsen JE,Brayer GD,Withers SG,McIntosh LP

更新日期：2001-08-28 00:00:00

abstract：:The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...

journal_title：Biochemistry

authors： Ramsey AJ,Fitzpatrick PF

更新日期：1998-06-23 00:00:00

abstract：:The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 h...

journal_title：Biochemistry

authors： Balhorn R,Jackson V,Granner D,Chalkey R

更新日期：1975-06-03 00:00:00

abstract：:Amyloid beta-protein (Abeta) self-assembly is linked strongly to Alzheimer's disease. We found that PP-Leu, a tridecapeptide analogue of broad-spectrum antiviral peptides termed theta-defensins, potently inhibits Abeta oligomer and fibril formation. This effect appeared to be mediated through sequestration of the amyl...

journal_title：Biochemistry

authors： Yamin G,Ruchala P,Teplow DB

更新日期：2009-12-08 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...

journal_title：Biochemistry

authors： Thompson JE,Basarab GS,Andersson A,Lindqvist Y,Jordan DB

更新日期：1997-02-18 00:00:00

abstract：:Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...

journal_title：Biochemistry

authors： Strand L,Moe SE,Solbu TT,Vaadal M,Holen T

更新日期：2009-06-30 00:00:00

abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...

journal_title：Biochemistry

authors： Quantin B,Murphy G,Breathnach R

更新日期：1989-06-27 00:00:00