Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes.

abstract：:Malic enzyme from pigeon liver is alkylated by the substrate analogue bromopyruvate, resulting in the concomitant loss of its oxidative decarboxylase and oxalacetate decarboxylase activities, but not its ability to reduce alpha-keto acids. The inactivation of oxidative decarboxylase activity follows saturation kinetic...

journal_title：Biochemistry

authors： Chang GG,Hsu RY

更新日期：1977-01-25 00:00:00

abstract：:Total internal reflection fluorescence microscopy (TIRFM) has been used to directly measure the weak dissociation constants of IgG with a mouse IgG receptor (moFc gamma RII) that has been purified and reconstituted into substrate-supported planar membranes. Dissociation constants were measured for three different mous...

journal_title：Biochemistry

authors： Hsieh HV,Poglitsch CL,Thompson NL

更新日期：1992-11-24 00:00:00

abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...

journal_title：Biochemistry

authors： Tao T,Lamkin M

更新日期：1981-08-18 00:00:00

abstract：:Photolyases reverse the effects of UV light on cells by converting cyclobutane dipyrimidine photoproducts (pyrimidine dimers, Pyr mean value of Pyr) into pyrimidine monomers in a light-dependent reaction. Previous work has suggested that, based on substrate preference, there are two classes of photolyase: DNA photolya...

journal_title：Biochemistry

authors： Kim ST,Sancar A

更新日期：1991-09-03 00:00:00

abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

journal_title：Biochemistry

authors： Lin LF,Houng A,Reed GL

更新日期：2000-04-25 00:00:00

abstract：:The interactions between rhodopsin molecules in a micellar detergent solution (octyl glucoside) and in reconstituted phospholipid vesicles were studied in the dark and after bleaching. Resonance energy transfer measurements were used to monitor the proximity between rhodopsin monomers conjugated with a fluorescent don...

journal_title：Biochemistry

authors： Borochov-Neori H,Fortes PA,Montal M

更新日期：1983-01-04 00:00:00

abstract：:M proteins and other members of the M protein family, expressed on the surface of Streptococcus pyogenes, bind host proteins such as immunoglobulins, albumin, and fibrinogen. Protein H and the M1 protein are expressed by adjacent genes and both belong to the M protein family. In this work, the structure and stability ...

journal_title：Biochemistry

authors： Nilson BH,Frick IM,Akesson P,Forsén S,Björck L,Akerström B,Wikström M

更新日期：1995-10-17 00:00:00

abstract：:A striking feature of the CLIC (chloride intracellular channel) protein family is the ability of its members to convert between a soluble state and an integral membrane channel form. Direct evidence of the structural transition required for the CLIC protein to autonomously insert into the membrane is lacking, largely ...

journal_title：Biochemistry

authors： Goodchild SC,Howell MW,Littler DR,Mandyam RA,Sale KL,Mazzanti M,Breit SN,Curmi PM,Brown LJ

更新日期：2010-06-29 00:00:00

abstract：:The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the proteas...

journal_title：Biochemistry

authors： Fribourg C,Meijer AB,Mertens K

更新日期：2006-09-05 00:00:00

abstract：:Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...

journal_title：Biochemistry

authors： Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

更新日期：2006-02-28 00:00:00

abstract：:Carbamoylation and reductive methylation of tubulin have been shown previously to inhibit microtubule assembly, probably by attack on essential internal lysine residues [Mellado, W., Slebe, J., & Maccioni, R.B. (1982) Biochem. J. 203, 675-681; Szasz, J., Burns, R., & Sternlicht, H. (1982) J. Biol. Chem. 257, 3697-3704...

journal_title：Biochemistry

authors： Clark RW,Volpi M,Berlin RD

更新日期：1988-02-09 00:00:00

abstract：:Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseases. The synuclein family comprises three small naturally unfolded proteins implicated in neurodegenerative diseases and some forms of cancer. α-Synuclein is a soluble protein that forms toxic inclusions associated with ...

journal_title：Biochemistry

authors： Surgucheva I,Sharov VS,Surguchov A

更新日期：2012-06-12 00:00:00

abstract：:Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairin...

journal_title：Biochemistry

authors： Gamper HB Jr,Gewirtz A,Edwards J,Hou YM

更新日期：2004-08-10 00:00:00

abstract：:The propeptide binding/activation site on the vitamin K dependent carboxylase has been localized to a region of carboxylase between residues Arg +50 and Glu +225 by photoinactivation studies using [125I]tyrosyl-labeled benzoylphenylalanine (Bpa)-containing analogs of proFIX19, a peptide containing residues -18 to +1 o...

journal_title：Biochemistry

authors： Yamada M,Kuliopulos A,Nelson NP,Roth DA,Furie B,Furie BC,Walsh CT

更新日期：1995-01-17 00:00:00

abstract：:The two dominant glucolipids in Acholeplasma laidlawii, viz., 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDG) and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1----2)-O-alpha-D-glucopyranosyl ]- sn-glycerol (DGlcDG), have markedly different phase behavior. MGlcDG has an ability to form nonlamellar phases, wher...

journal_title：Biochemistry

authors： Eriksson PO,Rilfors L,Wieslander A,Lundberg A,Lindblom G

更新日期：1991-05-21 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:The structure of the His-94-->Cys variant of human carbonic anhydrase II (CAII) has been determined by X-ray crystallographic methods to a resolution of 2.3 A with a final crystallographic R factor of 0.155. This variant of CAII crystallizes in orthorhombic space group P2(1)2(1)2(1) which is the first example of a new...

journal_title：Biochemistry

authors： Alexander RS,Kiefer LL,Fierke CA,Christianson DW

更新日期：1993-02-16 00:00:00

abstract：:RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...

journal_title：Biochemistry

authors： Chen JL,VanEtten DM,Fountain MA,Yildirim I,Disney MD

更新日期：2017-07-11 00:00:00

abstract：:The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive...

journal_title：Biochemistry

authors： Evans TC Jr,Nelsestuen GL

更新日期：1994-11-15 00:00:00

abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

journal_title：Biochemistry

authors： Isambert MF,Gasnier B,Botton D,Henry JP

更新日期：1992-02-25 00:00:00

abstract：:Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...

journal_title：Biochemistry

authors： Huber RE,Parfett C,Woulfe-Flanagan H,Thompson DJ

更新日期：1979-09-18 00:00:00

abstract：:The Ogg1 protein of Saccharomyces cerevisiae belongs to a family of DNA glycosylases and apurinic/apyrimidinic site (AP) lyases, the signature of which is the alpha-helix-hairpin-alpha-helix-Gly/Pro-Asp (HhH-GPD) active site motif together with a conserved catalytic lysine residue, to which we refer as the HhH-GPD/K f...

journal_title：Biochemistry

authors： Guibourt N,Castaing B,Van Der Kemp PA,Boiteux S

更新日期：2000-02-22 00:00:00

abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...

journal_title：Biochemistry

authors： Cao H,Pietrak BL,Grubmeyer C

更新日期：2002-03-12 00:00:00

abstract：:The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...

journal_title：Biochemistry

authors： Song B,Cho JH,Raleigh DP

更新日期：2007-12-11 00:00:00

abstract：:Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...

journal_title：Biochemistry

authors： Shiozawa JA,Jelenska MM,Jacobson BS

更新日期：1987-07-28 00:00:00

abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...

journal_title：Biochemistry

authors： Orban-Glaß I,Voskoboynikova N,Busch KB,Klose D,Rickert C,Mosslehy W,Roder F,Wilkens V,Piehler J,Engelhard M,Steinhoff HJ,Klare JP

更新日期：2015-01-20 00:00:00

abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...

journal_title：Biochemistry

authors： Snitko Y,Han SK,Lee BI,Cho W

更新日期：1999-06-15 00:00:00

abstract：:The complete amino acid sequence was determined for bovine ubiquitin, and adenylate cyclase stimulating polypeptide, which is probably represented universally in living cells. Ubiquitin has a molecular weight of 8451 and consists of a single polypeptide chain containing 74 amino acid residues. It contains four argini...

journal_title：Biochemistry

authors： Schlesinger DH,Goldstein G,Niall HD

更新日期：1975-05-20 00:00:00

abstract：:A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...

journal_title：Biochemistry

authors： Wang ZF,Yang J,Nie ZQ,Wu M

更新日期：1991-01-29 00:00:00

abstract：:Aminoacyl-tRNA synthetases contain one or three Mg(2+) ions in their catalytic sites. In addition to their role in ATP binding, these ions are presumed to play a role in catalysis by increasing the electropositivity of the alpha-phosphate and stabilizing the pentavalent transition state. In the class II aaRS, two high...

journal_title：Biochemistry

authors： Ador L,Jaeger S,Geslain R,Martin F,Cavarelli J,Eriani G

更新日期：2004-06-08 00:00:00