Direct measurement of the weak interactions between a mouse Fc receptor (Fc gamma RII) and IgG1 in the absence and presence of hapten: a total internal reflection fluorescence microscopy study.

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00

abstract：:Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...

journal_title：Biochemistry

authors： Prakobphol A,Thomsson KA,Hansson GC,Rosen SD,Singer MS,Phillips NJ,Medzihradszky KF,Burlingame AL,Leffler H,Fisher SJ

更新日期：1998-04-07 00:00:00

abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

journal_title：Biochemistry

authors： Cloherty EK,Levine KB,Carruthers A

更新日期：2001-12-25 00:00:00

abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...

journal_title：Biochemistry

authors： Yoder G,Pancoska P,Keiderling TA

更新日期：1997-12-09 00:00:00

abstract：:OleD is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in Stenotrophomonas maltophilia. The gene coding for OleD clusters with three other genes, oleABC, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. In this stu...

journal_title：Biochemistry

authors： Bonnett SA,Papireddy K,Higgins S,del Cardayre S,Reynolds KA

更新日期：2011-11-08 00:00:00

abstract：:The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...

journal_title：Biochemistry

authors： Liuzzi M,Weinfeld M,Paterson MC

更新日期：1987-06-16 00:00:00

abstract：:We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...

journal_title：Biochemistry

authors： Callender R,Chen D,Lugtenburg J,Martin C,Rhee KW,Sloan D,Vandersteen R,Yue KT

更新日期：1988-05-17 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...

journal_title：Biochemistry

authors： Hannibal L,Collins D,Brassard J,Chakravarti R,Vempati R,Dorlet P,Santolini J,Dawson JH,Stuehr DJ

更新日期：2012-10-30 00:00:00

abstract：:The ubiquitin signaling pathway consists of hundreds of enzymes that are tightly regulated for the maintenance of cell homeostasis. Parkin is an E3 ubiquitin ligase responsible for conjugating ubiquitin onto a substrate protein, which itself can be ubiquitinated. Ataxin-3 performs the opposing function as a deubiquiti...

journal_title：Biochemistry

authors： Bai JJ,Safadi SS,Mercier P,Barber KR,Shaw GS

更新日期：2013-10-22 00:00:00

abstract：:The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...

journal_title：Biochemistry

authors： Rehmann JP,Barton JK

更新日期：1990-02-20 00:00:00

abstract：:ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that sen...

journal_title：Biochemistry

authors： Liu T,Chen X,Ma Z,Shokes J,Hemmingsen L,Scott RA,Giedroc DP

更新日期：2008-10-07 00:00:00

abstract：:Incubation of bovine plasma amine oxidase (BPAO) with benzylamine and various p-substituted analogues results in a time-dependent inactivation that is attributable to buildup of the H(2)O(2)-turnover product on the basis of protection afforded by coincubation with catalase. The mechanism of inactivation is distinct fr...

journal_title：Biochemistry

authors： Lee Y,Shepard E,Smith J,Dooley DM,Sayre LM

更新日期：2001-01-23 00:00:00

abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...

journal_title：Biochemistry

authors： Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

更新日期：1999-10-05 00:00:00

abstract：:When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ...

journal_title：Biochemistry

authors： Ray WJ Jr,Long JW

更新日期：1976-09-07 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...

journal_title：Biochemistry

authors： Chen JL,VanEtten DM,Fountain MA,Yildirim I,Disney MD

更新日期：2017-07-11 00:00:00

abstract：:A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...

journal_title：Biochemistry

authors： Frankel AD,Ackers GK,Smith HO

更新日期：1985-06-04 00:00:00

abstract：:The incorporation of extensively fluorinated, or fluorous, analogues of hydrophobic amino acids into proteins potentially provides the opportunity to modulate the physicochemical properties of proteins in a predictable manner. On the basis of the properties of small fluorocarbon molecules, extensively fluorinated prot...

journal_title：Biochemistry

authors： Buer BC,de la Salud-Bea R,Al Hashimi HM,Marsh EN

更新日期：2009-11-17 00:00:00

abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

journal_title：Biochemistry

authors： Crute JJ,Wahl AF,Bambara RA

更新日期：1986-01-14 00:00:00

abstract：:The solution structure of the self-complementary dodecamer 5'd(CGCGPATTCGCG)2, containing a purine-thymine base pair within the hexameric canonical recognition site GAATTC for the restriction endonuclease EcoRI, is investigated by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Nonexchangeab...

journal_title：Biochemistry

authors： Clore GM,Oschkinat H,McLaughlin LW,Benseler F,Happ CS,Happ E,Gronenborn AM

更新日期：1988-05-31 00:00:00

abstract：:At pH 8.9 and 37 degrees C the half-times for tritium exchange with the C-2 protons of the histidines of trypsin are 73 days for His-57, and greater than 1000 days for His-40 and His-91. These half-times are much longer than the half-life of exchange for the C-2 proton of free histidine (2.8 days at pD 8.2), and longe...

journal_title：Biochemistry

authors： Krieger M,Koeppe RE 2nd,Stroud RM

更新日期：1976-08-10 00:00:00

abstract：:The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...

journal_title：Biochemistry

authors： Berndsen CE,Selleck W,McBryant SJ,Hansen JC,Tan S,Denu JM

更新日期：2007-02-27 00:00:00

abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

journal_title：Biochemistry

authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

更新日期：2004-09-07 00:00:00

abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...

journal_title：Biochemistry

authors： Plotnick MI,Mayne L,Schechter NM,Rubin H

更新日期：1996-06-11 00:00:00

abstract：:Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...

journal_title：Biochemistry

authors： Mowery PC,Lozier RH,Chae Q,Tseng YW,Taylor M,Stoeckenius W

更新日期：1979-09-18 00:00:00

abstract：:S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this com...

journal_title：Biochemistry

authors： Lee YT,Dimitrova YN,Schneider G,Ridenour WB,Bhattacharya S,Soss SE,Caprioli RM,Filipek A,Chazin WJ

更新日期：2008-10-14 00:00:00

abstract：:Escherichia coli were grown on 14.3% uniformly 13C-labeled glucose as the sole carbon source and challenged anaerobically with 90% 13C-labeled formaldehyde. The major multiply labeled metabolites were identified by 13C NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-prop...

journal_title：Biochemistry

authors： Hunter BK,Nicholls KM,Sanders JK

更新日期：1985-07-16 00:00:00

abstract：:Glucarate dehydratase (GlucD) from Pseudomonas putida catalyzes the dehydration of both (D)-glucarate and (L)-idarate to 3-deoxy-(L)-threo-2-hexulosarate as well as their epimerization. (D)-[6-13C]Glucarate and (L)-[6-13C]idarate have been synthesized for use in continuous assay of the reactions catalyzed by GlucD by ...

journal_title：Biochemistry

authors： Palmer DR,Hubbard BK,Gerlt JA

更新日期：1998-10-13 00:00:00

abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...

journal_title：Biochemistry

authors： Ziegler A,Seelig J

更新日期：2007-07-10 00:00:00