Abstract：
:The ubiquitin signaling pathway consists of hundreds of enzymes that are tightly regulated for the maintenance of cell homeostasis. Parkin is an E3 ubiquitin ligase responsible for conjugating ubiquitin onto a substrate protein, which itself can be ubiquitinated. Ataxin-3 performs the opposing function as a deubiquitinating enzyme that can remove ubiquitin from parkin. In this work, we have identified the mechanism of interaction between the ubiquitin-like (Ubl) domain from parkin and three C-terminal ubiquitin-interacting motifs (UIMs) in ataxin-3. (1)H-(15)N heteronuclear single-quantum coherence titration experiments revealed that there are weak direct interactions between all three individual UIM regions of ataxin-3 and the Ubl domain. Each UIM utilizes the exposed β-grasp surface of the Ubl domain centered around the I44 patch that did not vary in the residues involved or the surface size as a function of the number of ataxin-3 UIMs involved. Further, the apparent dissociation constant for ataxin-3 decreased as a function of the number of UIM regions used in experiments. A global multisite fit of the nuclear magnetic resonance titration data, based on three identical binding ligands, resulted in a KD of 669 ± 62 μM for each site. Our observations support a multivalent ligand binding mechanism employed by the parkin Ubl domain to recruit multiple UIM regions in ataxin-3 and provide insight into how these two proteins function together in ubiquitination-deubiquitination pathways.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bai JJ,Safadi SS,Mercier P,Barber KR,Shaw GSdoi
10.1021/bi400780vsubject
Has Abstractpub_date
2013-10-22 00:00:00pages
7369-76issue
42eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0005456
更新日期：2000-08-01 00:00:00
abstract：:Kinetics studies in homogeneous aqueous solution showed that solubilized penicillin-binding protein 2a (sPBP2a) of methicillin-resistant Staphylococcus aureus (a bacterial DD-peptidase) was inhibited by the amphiphilic glycolipid antibiotic moenomycin. Inhibition at the peptidase site was determined by competition exp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982309p
更新日期：1999-08-10 00:00:00
abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0508688
更新日期：2006-01-31 00:00:00
abstract：:Distinct structural features of DNA, such as the curvature of dA tracts, are important in the recognition, packaging, and regulation of DNA. Physiologically relevant concentrations of magnesium have been found to enhance the curvature of dA tract DNAs, as monitored by solution-state NMR, indicating that the structure ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010853j
更新日期：2001-08-07 00:00:00
abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a011
更新日期：1982-01-19 00:00:00
abstract：:When Pseudomonas putida ATCC 39167 and plant-deleterious Pseudomonas fluorescens A225 were grown in an iron-deficient culture medium, they each produced two different novel yellow-green fluorescent pseudobactins: P39167-I, II and PA225-I, II. Pseudobactin P39167-I has a molecular formula of C46H65O23N13 and is monoani...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962670g
更新日期：1997-04-08 00:00:00
abstract：:Recently, a new clathrin assembly protein (AP180) has been purified from coated vesicles of bovine brain (Ahle & Ungewickell, 1986). This protein has been shown to promote polymerization of clathrin into a homogeneous population of baskets under conditions where pure clathrin does not polymerize by itself. We have pur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00416a040
更新日期：1988-08-09 00:00:00
abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00330a014
更新日期：1985-04-23 00:00:00
abstract：:The interaction of glutamyl-tRNA synthetase with tRNAGlu2 has been studied. The enzyme was purified to apparent homogeneity, and consists of a single chain with a molecular weight of 59 000. The sedimentation coefficient (sdegrees20,w) was found to be 3.7 S and suggests this enzyme is quite asymmetric. The enzyme bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a032
更新日期：1976-09-21 00:00:00
abstract：:Cytochrome c-553 from Desulfovibrio vulgaris exhibits a highly exposed heme and an unusually low reduction potential with respect to other c-type cytochromes. Solvent heme exposure has been indicated as one of the most important factors in modulating the midpoint potential of the redox center. To test this hypothesis,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025759x
更新日期：2002-07-09 00:00:00
abstract：:Rapid withdrawal of estrogen from immature chicks, previously stimulated with the hormone, results in the inhibition of transcription of mRNAs of egg white proteins, rapid degradation of existing estrogen-induced mRNAs of egg white proteins, and decline in ribosomes and weight of the oviduct. On rapid withdrawal of es...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00409a019
更新日期：1988-05-03 00:00:00
abstract：:A series of secreted proteases are included among the virulence factors documented for Staphylococcus aureus. In light of increasing antibiotic resistance of this dangerous human pathogen, these proteases are considered as suitable targets for the development of novel therapeutic strategies. The recent discovery of st...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035310j
更新日期：2003-11-25 00:00:00
abstract：:The committed step in menaquinone biosynthesis is the formation of o-succinylbenzoate (OSB). It is presumed to require the reaction of a seven-carbon intermediate of the shikimate pathway with a succinic semialdehyde-thiamin pyrophosphate (TPP) anion, derived by decarboxylation of 2-ketoglutarate. The following eviden...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00354a017
更新日期：1986-03-25 00:00:00
abstract：:Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1011755
更新日期：2010-12-07 00:00:00
abstract：:The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00392a033
更新日期：1987-09-08 00:00:00
abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00198a032
更新日期：1994-08-16 00:00:00
abstract：:PDZ domains are modular protein-protein interaction domains that bind to specific C-terminal sequences of membrane proteins and/or to other PDZ domains. Certain PDZ domains in PSD-95 and syntrophins interact with C-terminal peptide ligands and heterodimerize with the extended nNOS PDZ domain. The capacity to interact ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001633t
更新日期：2000-11-28 00:00:00
abstract：:DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00677
更新日期：2017-09-19 00:00:00
abstract：:NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a033
更新日期：1992-12-15 00:00:00
abstract：:The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00228a023
更新日期：1991-04-09 00:00:00
abstract：:Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0027581
更新日期：2001-03-27 00:00:00
abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992811p
更新日期：2000-06-13 00:00:00
abstract：:The highly conserved nature and tissue specificity of the seven vertebrate beta-tubulin isotypes provide circumstantial evidence that functional differences among isotypes may exist in vivo. Compelling evidence from studies of bovine brain beta-isotypes indicated significant conformational and functional differences i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972763d
更新日期：1998-03-31 00:00:00
abstract：:Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972612a
更新日期：1998-04-07 00:00:00
abstract：:Ks values for binding of selected substrates, competitive inhibitors, and a noncompetitive inhibitor were found to be similar for alpha-chymotrypsin and N-methyl-alpha-chymotrypsin. The rates and steps of binding of a competitive inhibitor and a noncompetitive inhibitor were also found to be similar for alpha-chymotry...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00262a017
更新日期：1982-09-14 00:00:00
abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100055m
更新日期：2010-05-11 00:00:00
abstract：:The association of nucleolar phosphoprotein C23 with preribosomal ribonucleoprotein (RNP) particles was examined in Novikoff hepatoma nucleoli. RNA was labeled with [3H]uridine for various times in cell suspensions, and RNP particles were extracted from isolated nucleoli and fractionated by sucrose gradient ultracentr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00368a063
更新日期：1986-10-07 00:00:00
abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971465d
更新日期：1997-10-14 00:00:00
abstract：:Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970380+
更新日期：1997-07-01 00:00:00
abstract：:To elucidate the role of the proline residue in the engineered signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae, we have remodeled an idealized signal sequence L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [Yamamoto, Y., Taniyama, Y., Kikuchi, M., & Ikehara, M. (1987) Biochem. Bio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00432a054
更新日期：1989-03-21 00:00:00
