A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus.

abstract：:Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...

journal_title：Biochemistry

authors： Parsons JL,Dianova II,Allinson SL,Dianov GL

更新日期：2005-08-09 00:00:00

abstract：:Translocation of DNA during in vitro DNA synthesis on nuclear matrix bound replicational assemblies from regenerating rat liver was determined by measuring the processivity (average number of nucleotides added following one productive binding event of the polymerase to the DNA template) of nuclear matrix bound DNA pol...

journal_title：Biochemistry

authors： Tubo RA,Martelli AM,Berezney R

更新日期：1987-09-08 00:00:00

abstract：:Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...

journal_title：Biochemistry

authors： Clarkson MW,Gilmore SA,Edgell MH,Lee AL

更新日期：2006-06-27 00:00:00

abstract：:The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the condition...

journal_title：Biochemistry

authors： Glick DM,Shalitin Y,Hilt CR

更新日期：1989-03-21 00:00:00

abstract：:Human frataxin has a vital role in the biosynthesis of iron-sulfur (Fe-S) clusters in mitochondria, and its deficiency causes the neurodegenerative disease Friedreich's ataxia. Proposed functions for frataxin in the Fe-S pathway include iron donation to the Fe-S cluster machinery and regulation of cysteine desulfurase...

journal_title：Biochemistry

authors： Gentry LE,Thacker MA,Doughty R,Timkovich R,Busenlehner LS

更新日期：2013-09-03 00:00:00

abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...

journal_title：Biochemistry

authors： Hayes GR,Williams AM,Lucas JJ,Enns CA

更新日期：1997-04-29 00:00:00

abstract：:Two-dimensional (2D) proton magnetic resonance techniques used in conjunction with laser photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy have been applied to studying the kringle 4 domain from human plasminogen at 360 MHz. Out of 11 potential CIDNP-sensitive aromatic side chains, only 5...

journal_title：Biochemistry

authors： De Marco A,Zetta L,Petros AM,Llinás M,Boelens R,Kaptein R

更新日期：1986-12-02 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...

journal_title：Biochemistry

authors： Serrano S,Huarte N,Rujas E,Andreu D,Nieva JL,Jiménez MA

更新日期：2017-10-17 00:00:00

abstract：:The mutants Arg54-->Leu, Arg54-->Lys, Arg305-->Lys, Asp309-->Glu, and Trp317-->Phe, located at subsites 1 and 2 in glucoamylase from Aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. As suggested ...

journal_title：Biochemistry

authors： Frandsen TP,Christensen T,Stoffer B,Lehmbeck J,Dupont C,Honzatko RB,Svensson B

更新日期：1995-08-15 00:00:00

abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...

journal_title：Biochemistry

authors： Huang HW

更新日期：2000-07-25 00:00:00

abstract：:The mechanism of the oxidative decarboxylation reaction catalyzed by the NAD-malic enzyme from Ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. Primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning rati...

journal_title：Biochemistry

authors： Karsten WE,Gavva SR,Park SH,Cook PF

更新日期：1995-03-14 00:00:00

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00

abstract：:Signal transducer and activator of transcription factors (STATs) are proteins that can translocate into the nucleus, bind DNA, and activate gene transcription. STAT proteins play a crucial role in cell proliferation, apoptosis, and differentiation. The prevalent view is that STAT proteins are able to form dimers and b...

journal_title：Biochemistry

authors： Sgrignani J,Olsson S,Ekonomiuk D,Genini D,Krause R,Catapano CV,Cavalli A

更新日期：2015-09-08 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is an intracellular receptor protein that regulates gene transcription in response to both man-made and natural ligands. A modular transactivaton domain (TAD) has been mapped to the 304 C-terminal amino acids and consists of acidic, Q-rich, and P/S/T-rich subdomains. We have used st...

journal_title：Biochemistry

authors： Watt K,Jess TJ,Kelly SM,Price NC,McEwan IJ

更新日期：2005-01-18 00:00:00

abstract：:Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...

journal_title：Biochemistry

authors： Habersetzer-Rochat C,Sampieri F

更新日期：1976-06-01 00:00:00

abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...

journal_title：Biochemistry

authors： Schurter EJ,Sayer JM,Oh-hara T,Yeh HJ,Yagi H,Luxon BA,Jerina DM,Gorenstein DG

更新日期：1995-07-18 00:00:00

abstract：:Phosphorescent probes are described that are quenchable by dioxygen and that partition into membranes. These probes are derivatives of porphyrin, in which the central metal, either zinc or palladium, induces intersystem crossing to enhance the triplet yield. The location of the probe in a suspension of membranes depen...

journal_title：Biochemistry

authors： Vanderkooi JM,Wright WW,Erecinska M

更新日期：1990-06-05 00:00:00

abstract：:The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...

journal_title：Biochemistry

authors： Heinrichs AA,Bortell R,Rahman S,Stein JL,Alnemri ES,Litwack G,Lian JB,Stein GS

更新日期：1993-10-26 00:00:00

abstract：:Protein disulfide isomerase (PDI) utilizes the active site sequence Cys-Gly-His-Cys (CGHC; E degrees ' = -180 mV) to effect thiol-disulfide interchange during oxidative protein folding. Here, the Cys-Gly-Cys-NH(2) (CGC) peptide is shown to have a disulfide reduction potential (E degrees ' = -167 mV) that is close to t...

journal_title：Biochemistry

authors： Woycechowsky KJ,Raines RT

更新日期：2003-05-13 00:00:00

abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...

journal_title：Biochemistry

authors： Bucci E,Fronticelli C,Gryczynski Z

更新日期：1991-04-02 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

journal_title：Biochemistry

authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

更新日期：2001-09-11 00:00:00

abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...

journal_title：Biochemistry

authors： Margalit H,Shapiro BA,Nussinov R,Owens J,Jernigan RL

更新日期：1988-07-12 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...

journal_title：Biochemistry

authors： Bowler BE,Dong A,Caughey WS

更新日期：1994-03-08 00:00:00

abstract：:An expression plasmid encoding the extracellular portion of the human tumor necrosis factor (TNF) type 1 receptor (TNF-R1) was constructed and used to generate a stable cell line secreting soluble TNF-R1 (sTNF-R1). The sTNF-R1 was purified, and its biochemical properties and its interactions with human TNF-alpha were ...

journal_title：Biochemistry

authors： Pennica D,Kohr WJ,Fendly BM,Shire SJ,Raab HE,Borchardt PE,Lewis M,Goeddel DV

更新日期：1992-02-04 00:00:00

abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...

journal_title：Biochemistry

authors： Kristensen T,Saris CJ,Hunter T,Hicks LJ,Noonan DJ,Glenney JR Jr,Tack BF

更新日期：1986-08-12 00:00:00

abstract：:Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...

journal_title：Biochemistry

authors： Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

更新日期：2013-08-13 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

journal_title：Biochemistry

authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

更新日期：1975-04-22 00:00:00