Kringle 4 from human plasminogen: a proton magnetic resonance study via two-dimensional photochemically induced dynamic nuclear polarization spectroscopy.

abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

journal_title：Biochemistry

authors： Pancoska P,Keiderling TA

更新日期：1991-07-16 00:00:00

abstract：:There are four isoforms of centrin in mammals, with variable sequence, tissue expression, and functional properties. We have recently characterized a number of structural, ion, and target binding properties of human centrin isoform HsCen2. This paper reports a similar characterization of HsCen3, overexpressed in Esche...

journal_title：Biochemistry

authors： Cox JA,Tirone F,Durussel I,Firanescu C,Blouquit Y,Duchambon P,Craescu CT

更新日期：2005-01-25 00:00:00

abstract：:Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...

journal_title：Biochemistry

authors： Milne SB,Ivanova PT,Armstrong MD,Myers DS,Lubarda J,Shulga YV,Topham MK,Brown HA,Epand RM

更新日期：2008-09-09 00:00:00

abstract：:Flavo-diiron proteins (FDPs) function as anaerobic nitric oxide scavengers in some microorganisms, catalyzing reduction of nitric to nitrous oxide. The FDP from Thermotoga maritima can be prepared in a deflavinated form with an intact diferric site (deflavo-FDP). Hayashi et al. [(2010) Biochemistry 49, 7040-7049] repo...

journal_title：Biochemistry

authors： Caranto JD,Weitz A,Giri N,Hendrich MP,Kurtz DM Jr

更新日期：2014-09-09 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The effect of the general anesthetics alpha-chloralose and chloral hydrate as well as the nonanesthetic beta-chloralose on the order of phosphatidylcholine and phosphatidylcholine-cholesterol liposomes has been examined by 2H nuclear magnetic resonance. Chloral hydrate interacts with the hydrophilic head-group region,...

journal_title：Biochemistry

authors： Forrest BJ,Mattai J

更新日期：1985-12-03 00:00:00

abstract：:The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....

journal_title：Biochemistry

authors： Grasser M,Christensen JM,Peterhänsel C,Grasser KD

更新日期：2007-05-29 00:00:00

abstract：:As part of a high-throughput, structural proteomic project we have used NMR spectroscopy to determine the solution structure and ascertain the function of a previously unknown, conserved protein (MtH895) from the thermophilic archeon Methanobacterium thermoautotrophicum. Our findings indicate that MtH895 contains a ce...

journal_title：Biochemistry

authors： Bhattacharyya S,Habibi-Nazhad B,Amegbey G,Slupsky CM,Yee A,Arrowsmith C,Wishart DS

更新日期：2002-04-16 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labile, trehalose-containing lipooligosaccharides (LOS). The structures from...

journal_title：Biochemistry

authors： Besra GS,McNeil MR,Khoo KH,Dell A,Morris HR,Brennan PJ

更新日期：1993-11-30 00:00:00

abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...

journal_title：Biochemistry

authors： Konkle ME,Muellner SK,Schwander AL,Dicus MM,Pokhrel R,Britt RD,Taylor AB,Hunsicker-Wang LM

更新日期：2009-10-20 00:00:00

abstract：:Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five importa...

journal_title：Biochemistry

authors： Stein RL,Strimpler AM,Hori H,Powers JC

更新日期：1987-03-10 00:00:00

abstract：:Homing endonucleases are distinguished by their ability to catalyze the cleavage of double-stranded DNA with extremely high specificity. I-PpoI endonuclease, a homing endonuclease from the slime mold Physarum polycephalum, is a small enzyme (2 x 20 kDa) of known three-dimensional structure that catalyzes the cleavage ...

journal_title：Biochemistry

authors： Mannino SJ,Jenkins CL,Raines RT

更新日期：1999-12-07 00:00:00

abstract：:A slow conversion of adenine residues to hypoxanthine occurs in single-stranded DNA when heated in neutral aqueous buffers. The rate of this reaction at pH 7.6 and 110 degrees C is k = 4 x 10(-8) s-1, as determined by base analysis of heat-treated DNA that contains radioactively labeled adenine residues. It is propose...

journal_title：Biochemistry

authors： Karran P,Lindahl T

更新日期：1980-12-23 00:00:00

abstract：:Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...

journal_title：Biochemistry

authors： Yokota K,Yamazaki I

更新日期：1977-05-03 00:00:00

abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

journal_title：Biochemistry

authors： Tripet BP,Goel A,Copie V

更新日期：2011-06-14 00:00:00

abstract：:Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...

journal_title：Biochemistry

authors： Munishkina LA,Henriques J,Uversky VN,Fink AL

更新日期：2004-03-23 00:00:00

abstract：:Aspartoacylase catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate and is the only brain enzyme that has been shown to effectively metabolize NAA. Although the exact role of this enzymatic reaction has not yet been completely elucidated, the metabolism of NAA appears to be nec...

journal_title：Biochemistry

authors： Le Coq J,An HJ,Lebrilla C,Viola RE

更新日期：2006-05-09 00:00:00

abstract：:We have previously shown that both recombinant apo(a) and native Lp(a) inhibit the binding of Glu-plasminogen to fibrin surfaces [Fleury & Anglés-Cano (1991) Biochemistry 30, 7630-7638; Rouy et al. (1992) Biochemistry 31, 6332-6339]. The aim of the present study was to characterize the mechanism of this inhibition and...

journal_title：Biochemistry

authors： Hervio L,Durlach V,Girard-Globa A,Anglés-Cano E

更新日期：1995-10-17 00:00:00

abstract：:During a recent investigation of the persistence of substrate ambiguity in contemporary enzymes, we identified three distinct ambiguous sugar kinases embedded within the modern Escherichia coli genome [Miller, B. G., and Raines, R. T. (2004) Biochemistry 43, 6387-6392]. These catalysts are the YajF, YcfX, and NanK pol...

journal_title：Biochemistry

authors： Miller BG,Raines RT

更新日期：2005-08-16 00:00:00

abstract：:In order to quantitate the contributions of the polar, uncharged amino acids to the stability of the native state of staphylococcal nuclease, each of the 13 alanines, 9 glycines, 9 threonines, 6 prolines, 6 glutamines, 6 asparagines, and 3 serines was substituted, either with both alanine and glycine or with 1 of thes...

journal_title：Biochemistry

authors： Green SM,Meeker AK,Shortle D

更新日期：1992-06-30 00:00:00

abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...

journal_title：Biochemistry

authors： Turchi JJ,Patrick SM,Henkels KM

更新日期：1997-06-17 00:00:00

abstract：:Efforts to elucidate the nature of the bimolecular interaction of parathyroid hormone (PTH) with its cognate receptor, the PTH receptor type 1 (PTHR1), have relied heavily on benzoylphenylalanine- (Bpa-) based photoaffinity cross-linking. However, given the flexibility, size, and shape of Bpa, the resolution at the PT...

journal_title：Biochemistry

authors： Monaghan P,Thomas BE,Woznica I,Wittelsberger A,Mierke DF,Rosenblatt M

更新日期：2008-06-03 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) catalyzes the acetyl-CoA-dependent acetylation of chloramphenicol by a ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates. Such a kinetic mechanism for a two-substrate reaction provides an opportunity to compare the affinity...

journal_title：Biochemistry

authors： Ellis J,Bagshaw CR,Shaw WV

更新日期：1991-11-05 00:00:00

abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...

journal_title：Biochemistry

authors： Childers MC,Daggett V

更新日期：2019-11-05 00:00:00

abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

journal_title：Biochemistry

authors： Sheardy RD,Winkle SA

更新日期：1989-01-24 00:00:00

abstract：:Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...

journal_title：Biochemistry

authors： Jayasena SD,Johnston BH

更新日期：1992-01-21 00:00:00

abstract：:Continuum methods were used to calculate the electrostatic contributions of charged and polar side chains to the overall stability of a small 41-residue helical protein, the peripheral subunit-binding domain. The results of these calculations suggest several residues that are destabilizing, relative to hydrophobic iso...

journal_title：Biochemistry

authors： Spector S,Wang M,Carp SA,Robblee J,Hendsch ZS,Fairman R,Tidor B,Raleigh DP

更新日期：2000-02-08 00:00:00

abstract：:We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...

journal_title：Biochemistry

authors： Watson DK,Moudrianakis EN

更新日期：1982-01-19 00:00:00

abstract：:Nitric oxide (NO) synthases (NOS) are thiolate-ligated heme-, tetrahydrobiopterin (BH(4))-, and flavin-containing monooxygenases which catalyze the NADPH-dependent conversion of L-arginine (L-Arg) to NO AND citrulline. NOS consists of two domains: an N-terminal oxygenase (heme- and BH(4)-bound) domain and a C-terminal...

journal_title：Biochemistry

authors： Sono M,Ledbetter AP,McMillan K,Roman LJ,Shea TM,Masters BS,Dawson JH

更新日期：1999-11-30 00:00:00