Abstract：
:Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five important mechanistic features for HLE. Few important remote subsite contacts are established in the Michaelis complex. Full recognition and tight binding of the substrate occurs in the transition state for acylation. The P3-S3 interaction is critical during acylation. Subsite contacts are unimportant in deacylation. P1 specificity is regulated by peptide length. An important steady-state kinetic consequence of this specificity is that the rate-limiting step of kc for p-nitroanilide hydrolysis changes from acylation to deacylation as the peptide chain is lengthened.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Stein RL,Strimpler AM,Hori H,Powers JCdoi
10.1021/bi00379a015subject
Has Abstractpub_date
1987-03-10 00:00:00pages
1301-5issue
5eissn
0006-2960issn
1520-4995journal_volume
26pub_type
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