Abstract:
:The crystal structures of the deoxy and carbonmonoxy forms of a distal pocket myoglobin mutant in which valine68(E11) is replaced by threonine have been solved to 2.1- and 2.2-A resolution, respectively. This substitution has been shown previously to cause large decreases in the rate of oxygen binding and to lower the equilibrium association constants for O2 and CO. The synchrotron Laue method was used for the rapid acquisition of X-ray diffraction data to overcome problems caused by the very rapid rate of autooxidation of the mutant protein. The refined deoxy structure shows that the noncoordinated water molecule in the distal pocket is in a position to form strong hydrogen bonds with both the N epsilon-H of the distal histidine64 and O gamma of threonine68 with no other unexpected alterations in the protein structure. In the carbonmonoxy form, the bound ligand is well-defined and inclined away from the two hydrogen-bonding groups, refining to a position in which the Fe-C-O angle is 162 degrees. This value is very close to that previously observed in recombinant wild-type and position-64 (E7) mutants of sperm whale myoglobin (160-170 degrees). The similarity of the CO conformations contrasts with the 150-fold range in equilibrium binding constants (KCO) among the distal pocket myoglobin mutants and indicates that CO affinities cannot be predicted from the coordination geometry of the bound ligand. Furthermore, a comparison of the infrared stretching frequencies of CO in wild-type, valine64 and threonine68 single mutant, and valine64-threonine68 double mutant pig carbonmonoxymyoglobins shows a lack of correlation between KCO and vCO. These effects can be understood in terms of the stability of noncovalently bound water in deoxymyoglobin and electrostatic interactions between bound ligands and the distal pocket residues.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cameron AD,Smerdon SJ,Wilkinson AJ,Habash J,Helliwell JR,Li T,Olson JSdoi
10.1021/bi00211a016subject
Has Abstractpub_date
1993-12-07 00:00:00pages
13061-70issue
48eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00578a015
更新日期:1979-05-29 00:00:00
abstract::The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00083a038
更新日期:1993-08-17 00:00:00
abstract::Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049127h
更新日期:2004-08-17 00:00:00
abstract::Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a033
更新日期:1983-04-12 00:00:00
abstract::The primary structure of the J chain from a human Waldenströms IgM protein has been determined using a combination of automated and conventional Edman degradative procedures. Eighty-five percent of the sequence was established with peptides isolated from tryptic digests of carboxyamidomethylated and citraconylated J c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a002
更新日期:1977-08-09 00:00:00
abstract::The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990431y
更新日期:1999-08-24 00:00:00
abstract::Complete 1H NMR sequential assignments have been made for copper(I)- and silver (I)-substituted metallothionein (MT) from Saccharomyces cerevisiae using standard 2D 1H NMR methods. The fingerprint region of the COSY spectrum of both metalloproteins shows a doubling of a few backbone proton resonances from residue K41 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00077a032
更新日期:1993-07-06 00:00:00
abstract::The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00479a022
更新日期:1990-07-10 00:00:00
abstract::A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00452a026
更新日期:1989-12-26 00:00:00
abstract::The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00227a024
更新日期:1991-04-02 00:00:00
abstract::Peptide nucleic acids (PNAs) are nonionic DNA/RNA mimics that can recognize complementary sequences by Watson-Crick base pairing. The neutral PNA backbone facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs) can be important tools for exploring the structure and function...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700230a
更新日期:2007-06-26 00:00:00
abstract::Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047773a
更新日期:2005-04-05 00:00:00
abstract::Homoisocitrate dehydrogenase (HIcDH, 3-carboxy-2-hydroxyadipate dehydrogenase) catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD as an oxidizing agent. A chemical mechanism for HIcDH is proposed on the basis of the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702361j
更新日期:2008-04-01 00:00:00
abstract::ABCG2 is a half-ATP binding cassette (ABC) drug transporter that consists of a nucleotide binding domain (NBD) followed by a transmembrane domain. This half-ABC transporter is thought to form a homodimer in the plasma membrane where it transports anticancer drugs across the biological membranes in an ATP-dependent man...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900675v
更新日期:2009-09-29 00:00:00
abstract::Biofilms are surface-attached communities of bacteria enclosed in a polysaccharide matrix. Bacteria in a biofilm are extremely resistant to antibiotics. Several recent reports have linked the signaling molecule nitric oxide (NO) with biofilm dispersal. We have previously reported that an H-NOX (heme-nitric oxide/oxyge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401597m
更新日期:2014-04-08 00:00:00
abstract::Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a018
更新日期:1987-06-30 00:00:00
abstract::For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0255322
更新日期:2002-04-09 00:00:00
abstract::Acid beta-glucosidase is a lysosomal membrane protein that cleaves the O-beta-D-glucosidic linkage of glucosylceramide and aryl-beta-glucosides. Full activity reconstitution of the pure enzyme requires phospholipids and saposin C, an 80 aa activator protein. The deficiency of the enzyme or activator leads to Gaucher d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980785+
更新日期:1998-08-18 00:00:00
abstract::Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990286o
更新日期:1999-07-06 00:00:00
abstract::The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00203a004
更新日期:1994-09-20 00:00:00
abstract::The importance of a specific hydrogen bond between thermolysin and a phosphonamidate inhibitor, Z-NHCH2-PO(O-)-Leu-Leu (1) [Bartlett, P. A., & Marlowe, C. K. (1987) Science (Washington D.C.) 235, 569-571], has been reevaluated. We have determined the inhibition constants (binding free energies) for thermolysin of phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a006
更新日期:1989-06-13 00:00:00
abstract::The distances between the anticodon loops of fluorescent tRNAPhe bound to the E site and to either the A or the P site of poly(U)-programmed Escherichia coli ribosomes were measured by fluorescence energy transfer. Donor and acceptor molecules were wybutine and proflavin, respectively, both located 3' to the anticodon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a002
更新日期:1986-05-20 00:00:00
abstract::A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00584a026
更新日期:1979-08-21 00:00:00
abstract::Multivalent binding is an efficient means to enhance the affinity and specificity of chemical probes targeting multidomain proteins in order to study their function and role in disease. While the theory of multivalent binding is straightforward, physical and structural characterization of bivalent binding encounters m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00150
更新日期:2018-04-10 00:00:00
abstract::TOPORS is the first example of a protein that possesses both ubiquitin and SUMO E3 ligase activity. The ubiquitination activity maps to a conserved RING domain in the N-terminal region of the protein, which is not required for sumoylation activity. Similar to other E3 ligases, it is likely that the ubiquitin and sumoy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801904q
更新日期:2008-12-30 00:00:00
abstract::Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301369b
更新日期:2013-02-05 00:00:00
abstract::Prion diseases, also known as the transmissible spongiform encephalopathies (TSEs), are a group of fatal neurodegenerative disorders that affect humans and animals. These diseases are intimately associated with conformational conversion of the cellular prion protein, PrP(C), into an oligomeric beta-sheet-rich form, Pr...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi900108v
更新日期:2009-03-31 00:00:00
abstract::By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0269613
更新日期:2003-04-08 00:00:00
abstract::Structural properties of the isolated extrinsic regulatory 33 kDa protein of the water-oxidizing complex were analyzed at different pH values. It was found that (a) titrations of the buffer capacity reveal a characteristic hysteresis effect that is unique for the 33 kDa subunit and is not observed for the other extrin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963115h
更新日期:1997-05-27 00:00:00
abstract::Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0616706
更新日期:2006-11-28 00:00:00