Analysis of pH-induced structural changes of the isolated extrinsic 33 kilodalton protein of photosystem II.

abstract：:Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...

journal_title：Biochemistry

authors： Rim J,Oprian DD

更新日期：1995-09-19 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...

journal_title：Biochemistry

authors： Gao XL,Patel DJ

更新日期：1990-12-11 00:00:00

abstract：:The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...

journal_title：Biochemistry

authors： Lennick M,Brew SA,Ingham KC

更新日期：1985-05-07 00:00:00

abstract：:While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

journal_title：Biochemistry

authors： Xin X,Xi L,Tu SC

更新日期：1994-10-11 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...

journal_title：Biochemistry

authors： Castilho MS,Pavão F,Oliva G,Ladame S,Willson M,Périé J

更新日期：2003-06-17 00:00:00

abstract：:The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...

journal_title：Biochemistry

authors： Barr I,Stich TA,Gizzi AS,Grove TL,Bonanno JB,Latham JA,Chung T,Wilmot CM,Britt RD,Almo SC,Klinman JP

更新日期：2018-02-27 00:00:00

abstract：:The DNA binding domain of the mammalian glucocorticoid hormone receptor (GR) contains nine highly conserved cysteine residues, a conservation shared by the superfamily of steroid and thyroid hormone receptors. A fragment [150 amino acids (AA) in length] consisting of GR residues 407-556, containing within it the entir...

journal_title：Biochemistry

authors： Pan T,Freedman LP,Coleman JE

更新日期：1990-10-02 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:Polyalanine (polyA) is the third-most prevalent homopeptide repeat in eukaryotes, behind polyglutamine and polyasparagine. Abnormal expansion of the polyA repeat is linked to at least nine human diseases, and the disease mechanism likely involves enhanced length-dependent aggregation. Because of the simplicity of its ...

journal_title：Biochemistry

authors： Bernacki JP,Murphy RM

更新日期：2011-11-01 00:00:00

abstract：:Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...

journal_title：Biochemistry

authors： Chai W,Leteux C,Westling C,Lindahl U,Feizi T

更新日期：2004-07-06 00:00:00

abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

journal_title：Biochemistry

authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

更新日期：1985-12-17 00:00:00

abstract：:This paper defines the properties of interaction of endothelin-1 (Et-1) with cloned bovine ETA receptors. The Kd value of Et-1/ETA receptor complexes was estimated in membrane preparations to 20 pM using kinetic experiments and saturation experiments performed under quasi equilibrium conditions. Competition experiment...

journal_title：Biochemistry

authors： Desmarets J,Gresser O,Guedin D,Frelin C

更新日期：1996-11-26 00:00:00

abstract：:Apolipoprotein B (apoB) is the essential nonexchangeable protein in chylomicrons and very low-density lipoprotein-derived lipoprotein particles, including low-density lipoprotein (LDL). ApoB has been a key target for cardiovascular research because of its essential role in the assembly, secretion, delivery, and recept...

journal_title：Biochemistry

authors： Jiang ZG,Carraway M,McKnight CJ

更新日期：2005-02-01 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semialdehyde by the addition of H(2)O to the C-2, C-3 double bond, followed by the loss of HCl from the C-3 position. Sequence similarity between CaaD, an (alphabeta)(3) heterohexamer (molecular weight 47,547), and 4-oxalocr...

journal_title：Biochemistry

authors： Azurmendi HF,Wang SC,Massiah MA,Poelarends GJ,Whitman CP,Mildvan AS

更新日期：2004-04-13 00:00:00

abstract：:Sedimentation equilibrium and sedimentation velocity measurements demonstrate that turkey gizzard caldesmon is an elongated molecule of molecular mass 75 +/- 2 kDa. The frictional ratio (2.14) is consistent with a prolate ellipsoid of axial ratio 24, corresponding to an apparent length and width of 516 and 21.5 A, res...

journal_title：Biochemistry

authors： Malencik DA,Ausio J,Byles CE,Modrell B,Anderson SR

更新日期：1989-10-03 00:00:00

abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

journal_title：Biochemistry

authors： Pancoska P,Keiderling TA

更新日期：1991-07-16 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...

journal_title：Biochemistry

authors： Sigfridsson K,Young S,Hansson O

更新日期：1996-01-30 00:00:00

abstract：:Pokeweed antiviral protein (PAP) is a ribosome inactivating protein (RIP) that depurinates the sarcin/ricin loop (SRL) of rRNA, inhibiting protein synthesis. PAP depurinates viral RNA, and in doing so, lowers the infectivity of many plant viruses. The mechanism by which PAP accesses uncapped viral RNA is not known, im...

journal_title：Biochemistry

authors： Domashevskiy AV,Williams S,Kluge C,Cheng SY

更新日期：2017-11-14 00:00:00

abstract：:Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...

journal_title：Biochemistry

authors： Cosman M,Fiala R,Hingerty BE,Laryea A,Lee H,Harvey RG,Amin S,Geacintov NE,Broyde S,Patel D

更新日期：1993-11-23 00:00:00

abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

journal_title：Biochemistry

authors： Rice HC,Young-Pearse TL,Selkoe DJ

更新日期：2013-05-14 00:00:00

abstract：:The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...

journal_title：Biochemistry

authors： Rodday SM,Webber AN,Bingham SE,Biggins J

更新日期：1995-05-16 00:00:00

abstract：:Electron transfer between the Rieske iron-sulfur protein (Fe(2)S(2)) and cytochrome c(1) was studied using the ruthenium dimer, Ru(2)D, to either photoreduce or photooxidize cytochrome c(1) within 1 micros. Ru(2)D has a charge of +4, which allows it to bind with high affinity to the cytochrome bc(1) complex. Flash pho...

journal_title：Biochemistry

authors： Sadoski RC,Engstrom G,Tian H,Zhang L,Yu CA,Yu L,Durham B,Millett F

更新日期：2000-04-18 00:00:00

abstract：:Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...

journal_title：Biochemistry

authors： Perez-Ramirez B,Shearwin KE,Timasheff SN

更新日期：1994-05-24 00:00:00

abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...

journal_title：Biochemistry

authors： Zambelli B,Musiani F,Savini M,Tucker P,Ciurli S

更新日期：2007-03-20 00:00:00

abstract：:Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...

journal_title：Biochemistry

authors： Lee WC,Lin WL,Matsui T,Chen ES,Wei TY,Lin WH,Hu H,Zheng YG,Tsai MD,Ho MC

更新日期：2015-12-29 00:00:00

abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...

journal_title：Biochemistry

authors： Marinetti TD,Snyder GH,Sykes BD

更新日期：1977-02-22 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

journal_title：Biochemistry

authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

更新日期：2005-02-15 00:00:00