Cadmium-113 NMR studies of the DNA binding domain of the mammalian glucocorticoid receptor.

Abstract:

:The DNA binding domain of the mammalian glucocorticoid hormone receptor (GR) contains nine highly conserved cysteine residues, a conservation shared by the superfamily of steroid and thyroid hormone receptors. A fragment [150 amino acids (AA) in length] consisting of GR residues 407-556, containing within it the entire DNA binding domain (residues 440-525), has been overexpressed and purified from Escherichia coli previously. This fragment has been shown to contain 2.3 +/- 0.2 mol of Zn(II) per mole of protein [Freedman, L. P., Luisi, B. F., Korszun, Z. R., Basavappa, R., Sigler, P. B., & Yamamoto, K. R. (1988) Nature 334, 543]. Zn(II) [or Cd(II) substitution] has been shown to be essential for specific DNA binding. 113Cd NMR of a cloned construct containing the minimal DNA binding domain of 86 AA residues [denoted GR(440-525)] with 113Cd(II) substituted for Zn(II) identifies 2 Cd(II) binding sites by the presence of 2 113Cd NMR signals each of which integrates to 1 113Cd nucleus. The chemical shifts of these two sites, 704 and 710 ppm, suggest that each 113Cd(II) is coordinated to four isolated -S- ligands. Shared -S- ligands connecting the two 113Cd(II) ions do not appear to be present, since their T1s differ by 10-fold, 0.2 and 2.0 s, respectively. Addition of a third 113Cd(II) or Zn(II) to 113Cd2GR(440-525) results in occupancy of a third site, which introduces exchange modulation of the two original 113Cd NMR signals causing them to disappear. Addition of EDTA to the protein restores the original two signals.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Pan T,Freedman LP,Coleman JE

doi

10.1021/bi00491a016

subject

Has Abstract

pub_date

1990-10-02 00:00:00

pages

9218-25

issue

39

eissn

0006-2960

issn

1520-4995

journal_volume

29

pub_type

杂志文章
  • Structural and dynamic aspects of binding of a prototype lexitropsin to the decadeoxyribonucleotide d(CGCAATTGCG)2 deduced from high-resolution 1H NMR Studies.

    abstract::Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00401a066

    authors: Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

    更新日期:1988-01-12 00:00:00

  • Formaldehyde metabolism by Escherichia coli. Carbon and solvent deuterium incorporation into glycerol, 1,2-propanediol, and 1,3-propanediol.

    abstract::Escherichia coli were grown on 14.3% uniformly 13C-labeled glucose as the sole carbon source and challenged anaerobically with 90% 13C-labeled formaldehyde. The major multiply labeled metabolites were identified by 13C NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-prop...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00336a050

    authors: Hunter BK,Nicholls KM,Sanders JK

    更新日期:1985-07-16 00:00:00

  • Thermodynamic mapping of effector protein interfaces with RalA and RalB.

    abstract::RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi501530u

    authors: Campbell LJ,Peppa M,Crabtree MD,Shafiq A,McGough NF,Mott HR,Owen D

    更新日期:2015-02-17 00:00:00

  • CO-induced structural rearrangement of the C cluster in Carboxydothermus hydrogenoformans CO dehydrogenase-evidence from Ni K-edge X-ray absorption spectroscopy.

    abstract::We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036104e

    authors: Gu W,Seravalli J,Ragsdale SW,Cramer SP

    更新日期:2004-07-20 00:00:00

  • Isolation and characterization of bovine factor XII (Hageman factor).

    abstract::Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00629a036

    authors: Fujikawa K,Walsh KA,Davie EW

    更新日期:1977-05-17 00:00:00

  • Investigation into the catalytic role for the tryptophan residues within domain III of Pseudomonas aeruginosa exotoxin A.

    abstract::The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi961985t

    authors: Beattie BK,Prentice GA,Merrill AR

    更新日期:1996-12-03 00:00:00

  • Molecular dynamics simulations of beta-ketoacyl-, beta-hydroxyacyl-, and trans-2-enoyl-acyl carrier proteins of Escherichia coli.

    abstract::Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi901713r

    authors: Chan DI,Tieleman DP,Vogel HJ

    更新日期:2010-04-06 00:00:00

  • Structural coupling between the oxygen-evolving Mn cluster and a tyrosine residue in photosystem II as revealed by Fourier transform infrared spectroscopy.

    abstract::The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971760y

    authors: Noguchi T,Inoue Y,Tang XS

    更新日期:1997-12-02 00:00:00

  • Oxygen equilibria of Octopus dofleini hemocyanin.

    abstract::Oxygen binding by Octopus dofleini hemocyanin was examined under very nearly physiological conditions. The effects of pH, ionic composition, temperature, and aggregation were controlled so that the role each plays in modulating oxygen binding can be isolated. There is a very large effect of pH on affinity, the Bohr ef...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00338a015

    authors: Miller KI

    更新日期:1985-08-13 00:00:00

  • Simulation-based prediction of phosphatidylinositol 4,5-bisphosphate binding to an ion channel.

    abstract::Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi301350s

    authors: Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

    更新日期:2013-01-15 00:00:00

  • Conformational changes in the cytoplasmic domain of the Escherichia coli aspartate receptor upon adaptive methylation.

    abstract::By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980343y

    authors: Le Moual H,Quang T,Koshland DE Jr

    更新日期:1998-10-20 00:00:00

  • Synthesis and inhibition mechanism of Delta lac-acetogenins, a novel type of inhibitor of bovine heart mitochondrial complex I.

    abstract::We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0479003

    authors: Ichimaru N,Murai M,Abe M,Hamada T,Yamada Y,Makino S,Nishioka T,Makabe H,Makino A,Kobayashi T,Miyoshi H

    更新日期:2005-01-18 00:00:00

  • Nucleotide sequence of the gene for the gamma chain of human fibrinogen.

    abstract::A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00329a041

    authors: Rixon MW,Chung DW,Davie EW

    更新日期:1985-04-09 00:00:00

  • Retinoschisin (RS1) interacts with negatively charged lipid bilayers in the presence of Ca2+: an atomic force microscopy study.

    abstract::Retinoschisin (RS1) is a retina-specific secreted protein encoding a conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in the X-linked retinoschisis gene (RS1) cause X-linked reti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1007029

    authors: Kotova S,Vijayasarathy C,Dimitriadis EK,Ikonomou L,Jaffe H,Sieving PA

    更新日期:2010-08-24 00:00:00

  • Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2',3'-dideoxynucleotide terminators.

    abstract::Taq and T7 DNA polymerases have become basic molecular biology "tools" for DNA sequence analysis. However, Taq, unlike T7 DNA polymerase, is strongly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very different substrate selectivities. Equilibrium binding and rate consta...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi951682j

    authors: Brandis JW,Edwards SG,Johnson KA

    更新日期:1996-02-20 00:00:00

  • The hydrogen-bonding structure in parallel-stranded duplex DNA is reverse Watson-Crick.

    abstract::Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00226a012

    authors: Otto C,Thomas GA,Rippe K,Jovin TM,Peticolas WL

    更新日期:1991-03-26 00:00:00

  • Comparison of cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha-subunits and platelet glycoprotein IIb.

    abstract::The fibronectin receptor (FnR), the vitronectin receptor (VnR), and the platelet membrane glycoprotein (GP) IIb-IIIa complex are members of a family of cell adhesion receptors, which consist of noncovalently associated alpha- and beta-subunits. The present study was designed to compare the cDNA-derived protein sequenc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00399a021

    authors: Fitzgerald LA,Poncz M,Steiner B,Rall SC Jr,Bennett JS,Phillips DR

    更新日期:1987-12-15 00:00:00

  • A novel epsilon-cleavage within the transmembrane domain of the Alzheimer amyloid precursor protein demonstrates homology with Notch processing.

    abstract::Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi015794o

    authors: Weidemann A,Eggert S,Reinhard FB,Vogel M,Paliga K,Baier G,Masters CL,Beyreuther K,Evin G

    更新日期:2002-02-26 00:00:00

  • Phenylalanine hydroxylase: absolute configuration and source of oxygen of the 4a-hydroxytetrahydropterin species.

    abstract::The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00333a022

    authors: Dix TA,Bollag GE,Domanico PL,Benkovic SJ

    更新日期:1985-06-04 00:00:00

  • High-resolution crystal structure of manganese peroxidase: substrate and inhibitor complexes.

    abstract::Manganese peroxidase (MnP) is an extracellular heme enzyme that catalyzes the peroxide-dependent oxidation of Mn(II) to Mn(III). The Mn(III) is released from the enzyme in complex with oxalate. One heme propionate and the side chains of Glu35, Glu39, and Asp179 were identified as Mn(II) ligands in the 2.0 A resolution...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi047318e

    authors: Sundaramoorthy M,Youngs HL,Gold MH,Poulos TL

    更新日期:2005-05-03 00:00:00

  • Effect of monoclonal antibody binding on alpha-beta gamma subunit interactions in the rod outer segment G protein, Gt.

    abstract::The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00451a047

    authors: Mazzoni MR,Hamm HE

    更新日期:1989-12-12 00:00:00

  • Interaction of myosin subfragments with F-actin.

    abstract::The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00618a017

    authors: Margossian SS,Lowey S

    更新日期:1978-12-12 00:00:00

  • Kinetics of ATP-dependent Mg2+ flux in mitochondria.

    abstract::ATP-dependent Mg2+ accumulation in isolated mitochondria occurs predominantly in the matrix and inner membrane compartments. In mitochondria contaminated with lysosomes, the time course and magnitude of ATP-dependent Mg2+ accumulation are influenced by various cytoplasmic substances, besides substrates of the citric a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00656a013

    authors: Kun E

    更新日期:1976-06-01 00:00:00

  • Single-turnover kinetics of helicase-catalyzed DNA unwinding monitored continuously by fluorescence energy transfer.

    abstract::We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00251a044

    authors: Bjornson KP,Amaratunga M,Moore KJ,Lohman TM

    更新日期:1994-11-29 00:00:00

  • Induction by torsional stress of an altered DNA conformation 5' upstream of the gene for a high mobility group protein from trout and specific binding to flanking sequences by the gene product HMG-T.

    abstract::We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00402a012

    authors: Wright JM,Dixon GH

    更新日期:1988-01-26 00:00:00

  • Catalysis of RNA cleavage by a ribozyme derived from the group I intron of Anabaena pre-tRNA(Leu).

    abstract::In the cyanobacterium Anabaena PCC7120, the precursor to tRNA(Leu) contains a 249-nucleotide group I intron that undergoes efficient self-splicing in vitro. By deleting the 5' and 3' splice sites, this intron has now been converted to an RNA enzyme that uses a guanosine nucleophile to cleave substrate RNAs (S) with mu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00253a033

    authors: Zaug AJ,Dávila-Aponte JA,Cech TR

    更新日期:1994-12-13 00:00:00

  • Functional groups at the catalytic site of BF1 adenosinetriphosphatase from Escherichia coli.

    abstract::The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00531a011

    authors: Ting LP,Wang JH

    更新日期:1982-01-19 00:00:00

  • Direct visualization of the binding of c-Myc/Max heterodimeric b-HLH-LZ to E-box sequences on the hTERT promoter.

    abstract::Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi700076m

    authors: Lebel R,McDuff FO,Lavigne P,Grandbois M

    更新日期:2007-09-11 00:00:00

  • Inhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase.

    abstract::Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi7016427

    authors: Korman TP,Tan YH,Wong J,Luo R,Tsai SC

    更新日期:2008-02-19 00:00:00

  • Dioldehydrase: an essential role for potassium ion in the homolytic cleavage of the cobalt-carbon bond in adenosylcobalamin.

    abstract::The complex of dioldehydrase with adenosylcobalamin (coenzyme B12) and potassium ion reacts with molecular oxygen in the absence of a substrate to oxidize coenzyme and inactivate the complex. In this article, high performance liquid chromatography and mass spectral analysis are used to identify the nucleoside products...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi700078z

    authors: Schwartz PA,Frey PA

    更新日期:2007-06-19 00:00:00