Inhibiting gene expression with peptide nucleic acid (PNA)--peptide conjugates that target chromosomal DNA.

abstract：:Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...

journal_title：Biochemistry

authors： Latruffe N,Brenner SC,Fleischer S

更新日期：1980-11-11 00:00:00

abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

journal_title：Biochemistry

authors： Glaser PE,Gross RW

更新日期：1995-09-26 00:00:00

abstract：:Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...

journal_title：Biochemistry

authors： Rudolph R,Fuchs I,Jaenicke R

更新日期：1986-04-08 00:00:00

abstract：:The binding of ATP to pertussis toxin and its components, the A subunit and B oligomer, was investigated. Whereas, radiolabeled ATP bound to the B oligomer and pertussis toxin, no binding to the A subunit was observed. The binding of [3H]ATP to pertussis toxin and the B oligomer was inhibited by nucleotides. The relat...

journal_title：Biochemistry

authors： Hausman SZ,Manclark CR,Burns DL

更新日期：1990-07-03 00:00:00

abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...

journal_title：Biochemistry

authors： Lee JS,Woodsworth ML,Latimer LJ

更新日期：1984-07-03 00:00:00

abstract：:A series of double-helical oligodeoxyribonucleotides of sequence corresponding to a frame-shift mutational hot spot in the lambda CI gene, 5'-dGATGGGGCAG, are compared by proton magnetic resonance spectroscopy at 500 MHz of the exchangeable protons. Duplexes containing an extra guanine in a run of two, three, and four...

journal_title：Biochemistry

authors： Woodson SA,Crothers DM

更新日期：1988-01-12 00:00:00

abstract：:The oxidation of alcohol to aldehyde by horse liver alcohol dehydrogenase (LADH) requires the transfer of a hydride ion from the alcohol substrate to the cofactor nicotinamide adenine dinucleotide (NAD). A quantum mechanical tunneling contribution to this hydride transfer step has been demonstrated in a number of LADH...

journal_title：Biochemistry

authors： Colby TD,Bahnson BJ,Chin JK,Klinman JP,Goldstein BM

更新日期：1998-06-30 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Vpu is an integral membrane protein that belongs to the viroporin family. Viroporins interact with cell membranes, triggering membrane permeabilization and promoting release of viral particles. In vitro electrophysiological methods have revealed changes in membrane ion curre...

journal_title：Biochemistry

authors： Herrero L,Monroy N,González ME

更新日期：2013-01-08 00:00:00

abstract：:Modified (Arg63-Ile64 reactive-site peptide bond hydrolyzed) soybean trypsin inhibitor (Kunitz) with all reactive amino groups, except that of Ile64, protected was described in the preceding paper (Kowalski, D., and Laskowski, M., Jr. (1976), Biochemistry, preceding paper in this issue). Treatment of this inhibitor wi...

journal_title：Biochemistry

authors： Kowalski D,Laskowski M Jr

更新日期：1976-03-23 00:00:00

abstract：:The expression of apoE mRNA in the adrenal gland is inversely correlated to steroidogenesis and directly correlated to the level of cholesteryl ester stores. To further investigate the relationship between apoE and cellular cholesterol homeostasis, several parameters of cholesterol metabolism in the murine Y1 adrenal ...

journal_title：Biochemistry

authors： Prack MM,Rothblat GH,Erickson SK,Reyland ME,Williams DL

更新日期：1994-05-03 00:00:00

abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

journal_title：Biochemistry

authors： Satoh Y,Mori H,Ito K

更新日期：2003-06-24 00:00:00

abstract：:The molecular mechanism for the displacement of HMGA1 proteins from DNA is integral to disrupting their cellular function, which is linked to many metastatic cancers. Chemical shift and NOESY NMR experiments provide structural evidence for the displacement of an AT hook peptide (DNA binding motif of HMGA1 proteins) by...

journal_title：Biochemistry

authors： Smith AE,Buchmueller KL

更新日期：2011-09-27 00:00:00

abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...

journal_title：Biochemistry

authors： Muhn P,Fahr A,Hucho F

更新日期：1984-06-05 00:00:00

abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

journal_title：Biochemistry

authors： Sidik K,Smerdon MJ

更新日期：1990-08-14 00:00:00

abstract：:cDNAs encoding three cytochrome P-450 enzymes were cloned from a rabbit kidney cDNA library. These three cDNAs exhibit greater than 90% nucleotide sequence identity across the coding region. This degree of sequence identity is also seen with P450IVA4, an enzyme that catalyzes the omega-hydroxylation of prostaglandins ...

journal_title：Biochemistry

authors： Johnson EF,Walker DL,Griffin KJ,Clark JE,Okita RT,Muerhoff AS,Masters BS

更新日期：1990-01-30 00:00:00

abstract：:Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...

journal_title：Biochemistry

authors： Cogoi S,Codognotto A,Rapozzi V,Meeuwenoord N,van der Marel G,Xodo LE

更新日期：2005-08-09 00:00:00

abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...

journal_title：Biochemistry

authors： Wijffels G,Dalrymple BP,Prosselkov P,Kongsuwan K,Epa VC,Lilley PE,Jergic S,Buchardt J,Brown SE,Alewood PF,Jennings PA,Dixon NE

更新日期：2004-05-18 00:00:00

abstract：:A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...

journal_title：Biochemistry

authors： Flynn PF,Bieber Urbauer RJ,Zhang H,Lee AL,Wand AJ

更新日期：2001-06-05 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:The aromatic residues of fd coat protein in sodium dodecyl sulfate micelles are characterized by 1H and 13C NMR. Resonances from both types of nuclei show structure-induced chemical shift dispersion and line widths indicative of a folded native structure for the protein. The two tyrosines were found to have pKas of 12...

journal_title：Biochemistry

authors： Cross TA,Opella SJ

更新日期：1981-01-20 00:00:00

abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

journal_title：Biochemistry

authors： Chen JY,Waterman MR

更新日期：1992-03-03 00:00:00

abstract：:In Saccharomyces cerevisiae, the histidine-containing phosphotransfer (HPt) protein YPD1 transfers phosphoryl groups between the three different response regulator domains of SLN1, SSK1, and SKN7 (designated R1, R2, and R3, respectively). Together these proteins form a branched histidine-aspartic acid phosphorelay sys...

journal_title：Biochemistry

authors： Janiak-Spens F,Cook PF,West AH

更新日期：2005-01-11 00:00:00

abstract：:The operator-binding domain of phage lambda repressor provides a model for DNA recognition by the helix-turn-helix (HTH) motif. In the wild-type protein, dimerization is mediated by hydrophobic packing (of the dyad-related helix 5), which serves as an indirect determinant of operator affinity. The mutant repressor, Ty...

journal_title：Biochemistry

authors： Benevides JM,Weiss MA,Thomas GJ Jr

更新日期：1991-05-07 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:We constructed and expressed chimeric receptor cDNAs with insulin receptor exon 3 (residues 191-297 of the cysteine-rich region) replaced with either the comparable region of the insulin-like growth factor I receptor (IGF-IR) or the insulin receptor related receptor (IRR). Both chimeric receptors still could bind insu...

journal_title：Biochemistry

authors： Zhang B,Roth RA

更新日期：1991-05-28 00:00:00

abstract：:We use laser flash photolysis and time-resolved Raman spectroscopy of CO-bound H93G myoglobin (Mb) mutants to study the influence of the proximal ligand on the CO rebinding kinetics. In H93G mutants, where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands (e.g., imidazole, 4-b...

journal_title：Biochemistry

authors： Cao W,Ye X,Sjodin T,Christian JF,Demidov AA,Berezhna S,Wang W,Barrick D,Sage JT,Champion PM

更新日期：2004-08-31 00:00:00

abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

journal_title：Biochemistry

authors： Rixon MW,Chung DW,Davie EW

更新日期：1985-04-09 00:00:00

abstract：:Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2)...

journal_title：Biochemistry

authors： Li W,Zhao C,Xia C,Antholine WE,Petering DH

更新日期：2001-06-26 00:00:00

abstract：:Enzyme therapeutics that can degrade l-methionine (l-Met) are of great interest as numerous malignancies are exquisitely sensitive to l-Met depletion. To exhaust the pool of methionine in human serum, we previously engineered an l-Met-degrading enzyme based on the human cystathionine-γ-lyase scaffold (hCGL-NLV) to cir...

journal_title：Biochemistry

authors： Yan W,Stone E,Zhang YJ

更新日期：2017-02-14 00:00:00

abstract：:Water deuteron NMR spectra have been studied for the system dipalmitoyllecithin (DPL)-heavy water (D2O) at different compositions and temperatures. From an analysis of the spectra in terms of quadrupole splittings, a phase diagram has been constructed for the temperature range 25-60 degrees C and the composition range...

journal_title：Biochemistry

authors： Ulmius J,Wennerström H,Lindblom G,Arvidson G

更新日期：1977-12-27 00:00:00