Abstract:
:The molecular mechanism for the displacement of HMGA1 proteins from DNA is integral to disrupting their cellular function, which is linked to many metastatic cancers. Chemical shift and NOESY NMR experiments provide structural evidence for the displacement of an AT hook peptide (DNA binding motif of HMGA1 proteins) by both monomeric and dimeric distamycin. However, the displaced AT hook alters distamycin binding by weakening the distamycin:DNA complex, while slowing monomeric distamycin dissociation when AT hook is in excess. The central role of the AT hook was evaluated by monitoring full-length HMGA1a protein binding using fluorescence anisotropy. HMGA1a was effectively displaced by distamycin, but the cooperative binding exhibited by distamycin was eliminated by displaced HMGA1a. Additionally, these studies indicate that HMGA1a is displaced from the DNA by 1 equiv of distamycin, suggesting the ability to develop therapeutics that take advantage of the positively cooperative nature of HMGA1a binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Smith AE,Buchmueller KLdoi
10.1021/bi200822csubject
Has Abstractpub_date
2011-09-27 00:00:00pages
8107-16issue
38eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Reductive dehalogenation such as that catalyzed by iodotyrosine deiodinase (IYD) is highly unusual in aerobic organisms but necessary for iodide salvage from iodotyrosine generated during thyroxine biosynthesis. Equally unusual is the dependence of this process on flavin. Rapid kinetics have now been used to define th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00410
更新日期:2015-07-28 00:00:00
abstract::The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00129a010
更新日期:1992-04-14 00:00:00
abstract::Although insulin and insulin-like growth factor-1 (IGF-1) belong to one family, insulin folds into one thermodynamically stable structure, while IGF-1-folds into two thermodynamically stable structures (native and swap forms). We have demonstrated previously that the bifurcating folding behavior of IGF-1 is mainly con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049710y
更新日期:2004-07-20 00:00:00
abstract::Rabbit aortic smooth muscle cells were evaluated for their ability to synthesize and accumulate glycosaminoglycans (GAGs). Because of the sensitivity of the microtechniques utilized, it is possible to determine the specific radioactivity of the GAGs obtained after radioactive incorporation of [35S]SO4(2-) and [14C]glu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00550a026
更新日期:1980-04-29 00:00:00
abstract::Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting its degradation by functioning as an E3 ubiquitin ligase. The primary p53 binding site on mdm2 is located in its N-terminal domain. Through binding to p53 at its N-terminal transactivation domain, mdm2 directly blocks the transcript...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060661u
更新日期:2006-08-01 00:00:00
abstract::A dimeric bis-benzimidazole molecule has been designed by computer modeling to bind to a DNA sequence via the DNA minor groove that covers a complete turn of B-DNA. A series of bis-benzimidazole dimers incorporating a -O-(CH(2))(n)()-X-CH(2))(n)()-O- linker, with n = 2 or 3 and X = O or N(+)H(Me), were screened for th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026926w
更新日期:2003-05-27 00:00:00
abstract::This study presents the first detailed examination by resonance Raman (RR) spectroscopy of the rates of solvent exchange for the C5 and C3 positions of the TPQ cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from Hansenula polymorpha (HPAO). On the basis of crystal struc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011685y
更新日期:2002-01-15 00:00:00
abstract::The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00092
更新日期:2020-03-24 00:00:00
abstract::Peptide deformylase (EC 3.5.1.31) catalyzes the removal of a formyl group from the N-termini of nascent ribosome-synthesized polypeptides, an obligatory step during protein maturation in eubacteria. Since its discovery in crude Escherichia coli extracts 3 decades ago, the deformylase has resisted all attempts of purif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971155v
更新日期:1997-11-11 00:00:00
abstract::Memapsin 2 (beta-secretase) is the membrane-anchored aspartic protease that initiates the cleavage of beta-amyloid precursor protein (APP), leading to the production of amyloid-beta (Abeta), a major factor in the pathogenesis of Alzheimer's disease. The active site of memapsin 2 has been shown, with kinetic data and c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048106k
更新日期:2005-01-11 00:00:00
abstract::Azotobacter vinelandii bacterioferritin (AvBF) containing 800-1500 Co or Mn atoms as Co(III) and Mn(III) oxyhydroxide cores (Co-AvBF, Mn-AvBF) was synthesized by the same procedure used previously for horse spleen ferritin (HoSF). The kinetics of reduction of Co-AvBF and Mn-AvBF by ascorbic acid are first-order in eac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060164d
更新日期:2006-05-09 00:00:00
abstract::Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. The heme binding region has been localized to residues 1-385 of the beta1 subunit [beta1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972686m
更新日期:1998-03-31 00:00:00
abstract::Multiple intracellular receptors of the FK506 binding protein (FKBP) family of peptidylprolyl cis/trans-isomerases are potential targets for the immunosuppressive drug FK506. Inhibition of the protein phosphatase calcineurin (CaN), which has been implicated in the FK506-mediated blockade of T cell proliferation, was s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061616p
更新日期:2006-12-26 00:00:00
abstract::In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. In rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00409a025
更新日期:1988-05-03 00:00:00
abstract::The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300886q
更新日期:2013-01-08 00:00:00
abstract::Heme A, an obligatory cofactor in eukaryotic cytochrome c oxidase, is produced from heme B (protoheme) via two enzymatic reactions catalyzed by heme O synthase and heme A synthase. Heme O synthase is responsible for the addition of a farnesyl moiety, while heme A synthase catalyzes the oxidation of a methyl substituen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0203536
更新日期:2002-09-10 00:00:00
abstract::The folding behavior of bovine growth hormone (bGH) is examined by chemical and pH denaturation using several spectroscopic probes of protein secondary and tertiary structure. Partially denaturing concentrations of urea eliminate the native-state quenching of intrinsic tryptophan fluorescence, from the single protein ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00457a022
更新日期:1990-02-06 00:00:00
abstract::The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001071d
更新日期:2000-12-12 00:00:00
abstract::Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer sol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00530a011
更新日期:1982-01-05 00:00:00
abstract::Retinal membrane guanylyl cyclase (RetGC) in the outer segments of vertebrate photoreceptors is controlled by guanylyl cyclase activating proteins (GCAPs), responding to light-dependent changes of the intracellular Ca(2+) concentrations. We present evidence that a different RetGC binding protein, retinal degeneration ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201342b
更新日期:2011-11-08 00:00:00
abstract::Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a024
更新日期:1977-05-03 00:00:00
abstract::Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960132y
更新日期:1996-08-13 00:00:00
abstract::The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01072
更新日期:2018-12-11 00:00:00
abstract::One- and two-dimensional NMR experiments were carried out on a decamer, d-(CGCTTTTCGC).d(GCGAAAAGCG), and on the same sequence with the addition of an unpaired thymidine, d(CGCTTTTCGC).d(GCGAATAAGCG), which will be referred to as the T-bulge decamer. Evidence from one-dimensional NOE experiments on the exchangeable pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00489a047
更新日期:1990-09-18 00:00:00
abstract::Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00552a023
更新日期:1980-05-27 00:00:00
abstract::Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found, for example, in such macrolide antibiotics as erthyromycin, azithromycin, and clarithromycin. The efficacies of these macrolide antibiotics are markedly reduced in the absence of desosamine. In the bacterium Streptomyces venezuelae, six enzymes are required...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700751d
更新日期:2007-08-07 00:00:00
abstract::2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00163a036
更新日期:1992-12-08 00:00:00
abstract::A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a008
更新日期:1994-06-14 00:00:00
abstract::Life span regulation and inhibition of gene silencing in yeast have been linked to nicotinamide effects on Sir2 enzymes. The Sir2 enzymes are NAD(+)-dependent protein deacetylases that influence gene expression by forming deacetylated proteins, nicotinamide and 2'-O-acetyl-ADPR. Nicotinamide is a base-exchange substra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034959l
更新日期:2003-08-12 00:00:00
abstract::The recently identified Krokinobacter rhodopsin 2 (KR2) functions as a light-driven sodium ion pump. The structure of the retinal-binding pocket of KR2 offers important insights into the mechanisms of KR2, which has motif of Asn112, Asp116, and Gln123 (NDQ) that is common among sodium ion pump rhodopsins but is unique...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00999
更新日期:2017-01-31 00:00:00