Binding isotherms of nucleosides and polynucleotides measured by continuous ultrafiltration at constant volume: a rapid and precise technique.

abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...

journal_title：Biochemistry

authors： Zaug AJ,McEvoy MM,Cech TR

更新日期：1993-08-10 00:00:00

abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...

journal_title：Biochemistry

authors： Ebneth A,Schweers O,Thole H,Fagin U,Urbanke C,Maass G,Wolfes H

更新日期：1994-12-06 00:00:00

abstract：:We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...

journal_title：Biochemistry

authors： Watson DK,Moudrianakis EN

更新日期：1982-01-19 00:00:00

abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...

journal_title：Biochemistry

authors： Suzuki A,Ito T

更新日期：1996-04-23 00:00:00

abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...

journal_title：Biochemistry

authors： Mayer M,Schiffer S,Marchfelder A

更新日期：2000-02-29 00:00:00

abstract：:Comparative primary structural analyses have begun to elucidate polymorphic residues and segments of the class I antigens of the major histocompatibility complex, at least some of which presumably contribute to determinants important in immune recognition events. HLA-A2 structural variants have been described which ar...

journal_title：Biochemistry

authors： Krangel MS,Taketani S,Biddison WE,Strong DM,Strominger JL

更新日期：1982-11-23 00:00:00

abstract：:Effects of different end sequences on melting, circular dichroism spectra (CD), and enzyme binding properties were investigated for four 40 base pair, non-self-complementary duplex DNA oligomers. The center sequences of these oligoduplexes have either of two 22 base pair modules flanked on both sides by sequences diff...

journal_title：Biochemistry

authors： Vallone PM,Benight AS

更新日期：2000-07-04 00:00:00

abstract：:The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In this study, a method for expanding the genetic code of this cyanobacterium has been established, thereby allowing the incorporation of unn...

journal_title：Biochemistry

authors： Chemla Y,Friedman M,Heltberg M,Bakhrat A,Nagar E,Schwarz R,Jensen MH,Alfonta L

更新日期：2017-04-25 00:00:00

abstract：:Protein farnesyltransferase catalyzes the posttranslational farnesylation of several proteins involved in signal transduction, including Ras, and is a target enzyme for antitumor therapies. Efficient product formation catalyzed by protein farnesyltransferase requires an enzyme-bound zinc cation and high concentrations...

journal_title：Biochemistry

authors： Saderholm MJ,Hightower KE,Fierke CA

更新日期：2000-10-10 00:00:00

abstract：:Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-assoc...

journal_title：Biochemistry

authors： Krylova OO,Pohl P

更新日期：2004-03-30 00:00:00

abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...

journal_title：Biochemistry

authors： Matsubara T,Iida M,Tsumuraya T,Fujii I,Sato T

更新日期：2008-07-01 00:00:00

abstract：:Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...

journal_title：Biochemistry

authors： Zhao B,Lamb DC,Lei L,Kelly SL,Yuan H,Hachey DL,Waterman MR

更新日期：2007-07-31 00:00:00

abstract：:Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum...

journal_title：Biochemistry

authors： Frederiksen DW

更新日期：1979-05-01 00:00:00

abstract：:Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...

journal_title：Biochemistry

authors： Schonhoft JD,Stivers JT

更新日期：2013-04-16 00:00:00

abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

journal_title：Biochemistry

authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

更新日期：2013-12-03 00:00:00

abstract：:ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...

journal_title：Biochemistry

authors： Roberts CS,Muralidharan S,Ni F,Mitra B

更新日期：2020-12-01 00:00:00

abstract：:We have analyzed the interactions of a histidine residue with a substrate using site-directed mutagenesis. Previous studies on tyrosyl-tRNA synthetase from Bacillus stearothermophilus have shown that a histidine residue (His-48) makes an interaction with ATP, which is improved on mutating Thr-51----Pro-51. We find on ...

journal_title：Biochemistry

authors： Lowe DM,Fersht AR,Wilkinson AJ,Carter P,Winter G

更新日期：1985-09-10 00:00:00

abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

journal_title：Biochemistry

authors： Rice HC,Young-Pearse TL,Selkoe DJ

更新日期：2013-05-14 00:00:00

abstract：:Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...

journal_title：Biochemistry

authors： Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

更新日期：1999-08-10 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:The heterotrimeric G protein subunit, alpha(s), can move reversibly from plasma membranes to cytoplasm in response to activation by GPCRs or activating mutations. We examined the importance of the unique N-terminus of alpha(s) in this translocation in cultured cells. alpha(s) contains a single site for palmitoylation ...

journal_title：Biochemistry

authors： Thiyagarajan MM,Bigras E,Van Tol HH,Hébert TE,Evanko DS,Wedegaertner PB

更新日期：2002-07-30 00:00:00

abstract：:A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...

journal_title：Biochemistry

authors： Dooijewaard G,Roossien FF,Robillard GT

更新日期：1979-07-10 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is als...

journal_title：Biochemistry

authors： Thauer RK

更新日期：2019-12-31 00:00:00

abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

journal_title：Biochemistry

authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

更新日期：1995-07-04 00:00:00

abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...

journal_title：Biochemistry

authors： Makde RD,Mahajan SK,Kumar V

更新日期：2007-02-27 00:00:00

abstract：:TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...

journal_title：Biochemistry

authors： Fu H,Alvarez MA,Khosla C,Bailey JE

更新日期：1996-05-28 00:00:00

abstract：:Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...

journal_title：Biochemistry

authors： Chai W,Leteux C,Westling C,Lindahl U,Feizi T

更新日期：2004-07-06 00:00:00

abstract：:Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...

journal_title：Biochemistry

authors： Forneris F,Heuts DP,Delvecchio M,Rovida S,Fraaije MW,Mattevi A

更新日期：2008-01-22 00:00:00

abstract：:The rubredoxin from Clostridium pasteurianum contains a single iron atom bound to the polypeptide chain by cysteines 6, 9, 39, and 42. The C42A variant of this protein has been prepared by site-directed mutagenesis and heterologous expression of the gene in Escherichia coli. The mutated protein was found to contain an...

journal_title：Biochemistry

authors： Meyer J,Gagnon J,Gaillard J,Lutz M,Achim C,Münck E,Pétillot Y,Colangelo CM,Scott RA

更新日期：1997-10-28 00:00:00