Abstract:
:We have analyzed the interactions of a histidine residue with a substrate using site-directed mutagenesis. Previous studies on tyrosyl-tRNA synthetase from Bacillus stearothermophilus have shown that a histidine residue (His-48) makes an interaction with ATP, which is improved on mutating Thr-51----Pro-51. We find on replacing His-48 in wild-type enzyme with either asparagine or glutamine that Asn-48 is equally as good as His-48 but His-48----Gln-48 leads to a far lower activity. The side chain of an asparagine residue may be superimposed on that of a histidine so that the amide-NH2 group of asparagine occupies the same position as the pi-N of histidine, whereas the equivalent -NH2 group of glutamine may be superimposed upon the tau-N. This suggests that it is the pi-N of histidine that hydrogen bonds with ATP and that there is no significant electrostatic interaction between the histidine and ATP. Incorporating the Pro-51 mutation into each of the Asn-48 and Gln-48 mutants gives an improvement in the affinity of the enzyme for ATP, but this improvement is less than that seen with the wild-type enzyme.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lowe DM,Fersht AR,Wilkinson AJ,Carter P,Winter Gdoi
10.1021/bi00340a022subject
Has Abstractpub_date
1985-09-10 00:00:00pages
5106-9issue
19eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900123j
更新日期:2009-11-03 00:00:00
abstract::The reaction center protein D1 in photosystem II shows a high turnover during illumination. The degradation of the D1 protein is preceded by photoinhibition of the electron transport in photosystem II. There are two distinct mechanisms for this: acceptor-side- and donor-side-induced photoinhibition. Here, donor-side-i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960083p
更新日期:1996-06-18 00:00:00
abstract::ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00770
更新日期:2020-12-01 00:00:00
abstract::Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and regulation of the tissue distribution of signaling molecules...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi6025424
更新日期:2007-04-03 00:00:00
abstract::A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025692d
更新日期:2002-06-25 00:00:00
abstract::Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00182
更新日期:2018-07-03 00:00:00
abstract::Electronic absorption, EPR, and resonance Raman spectroscopies revealed that CooA, the CO-sensing transcriptional regulator from Rhodospirillum rubrum, reacts with NO to form a five-coordinate NO-heme. NO must therefore displace both of the heme ligands from six-coordinate, low-spin Fe(II)CooA in forming five-coordina...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991378g
更新日期:2000-01-18 00:00:00
abstract::The respiratory chain of Vibrio cholerae contains three bd-type quinol oxygen reductases as well as one cbb(3) oxygen reductase. The cbb(3) oxygen reductase has been previously isolated and characterized; however, the natural mobile electron donor(s) that shuttles electrons between the bc(1) complex and the cbb(3) oxy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1004574
更新日期:2010-09-07 00:00:00
abstract::The plasma membrane of the Ehrlich ascites tumor cell contains an NADH dehydrogenase. This activity was shown not to be due to contamination by other subcellular membranes. A variety of electron acceptors have been compared as to rate with the following result: ferricyanide greater than cytochrome c greater than cytoc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00575a021
更新日期:1979-04-17 00:00:00
abstract::The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0509402
更新日期:2005-09-27 00:00:00
abstract::The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9605651
更新日期:1996-10-22 00:00:00
abstract::The light-sensing outer segments of photoreceptor cells harbor hundreds of flattened membranous discs containing the visual pigment, rhodopsin, and all the proteins necessary for visual signal transduction. PRCD (progressive rod-cone degeneration) protein is one of a few proteins residing specifically in photoreceptor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00489
更新日期:2016-09-13 00:00:00
abstract::Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972247n
更新日期:1998-05-05 00:00:00
abstract::Some properties of [4-vinyl] chlorophyllide a reductase are described. This enzyme converts divinyl chlorophyllide a to monovinyl chlorophyllide a. The latter is the immediate precursor of monovinyl chlorophyll a, the main chlorophyll in green plants. [4-Vinyl] chlorophyllide a reductase plays an important role in day...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00151a011
更新日期:1992-09-15 00:00:00
abstract::The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00519a024
更新日期:1981-08-04 00:00:00
abstract::The human endonuclease III homologue (hNTH1) removes premutagenic cytosine damage from DNA. This includes 5-hydroxycytosine, which has increased potential for pairing with adenine, resulting in C --> T transition mutations. Here we report that hNTH1 acts on both 5-hydroxycytosine and abasic sites preferentially when t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0028901
更新日期:2001-06-05 00:00:00
abstract::5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00377a042
更新日期:1987-02-10 00:00:00
abstract::We have previously shown functional differences in fibrinogen from hyperhomocysteinemic rabbits compared to that in control rabbits. This acquired dysfibrinogenemia is characterized by fibrin clots that are composed of abnormally thin, tightly packed fibers with increased resistance to fibrinolysis. Homocysteine thiol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052076j
更新日期:2006-02-28 00:00:00
abstract::SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9012462
更新日期:2009-11-24 00:00:00
abstract::The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00272a026
更新日期:1983-02-01 00:00:00
abstract::Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01347
更新日期:2016-04-12 00:00:00
abstract::A type II topoisomerase is essential for decatenating DNA replication products, and it accomplishes this task by passing one DNA duplex through a transient break in a second duplex. The B' domain of topoisomerase II contains three highly conserved motifs, EGDSA, PL(R/K)GK(I/L/M)LNVR, and IMTD(Q/A)DXD. We have investig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991328b
更新日期:2000-02-15 00:00:00
abstract::The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01199
更新日期:2017-01-17 00:00:00
abstract::The enzyme form of the calcium adenosinetriphosphatase of sarcoplasmic reticulum (CaATPase) that is stable in the presence of calcium, cE.Ca2, has a binding site for the catalytic Mg2+ ion with a dissociation constant of 0.94 +/- 0.15 mM at 25 degrees C, pH 7.0, and 100 mM KCl. This is approximately 10 times smaller t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00077a016
更新日期:1993-07-06 00:00:00
abstract::The Escherichia coli repressor of biotin biosynthesis is both a biotin ligase and the repressor of transcriptional initiation at the biotin biosynthetic operon. The small molecule, biotinyl-5'-adenylate (bio-5'-AMP), is the intermediate in the biotin ligation reaction and the positive allosteric effector for sequence-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a041
更新日期:1994-06-14 00:00:00
abstract::SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0490744
更新日期:2004-09-28 00:00:00
abstract::D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201378c
更新日期:2011-11-22 00:00:00
abstract::Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990599j
更新日期:1999-06-22 00:00:00
abstract::Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a041
更新日期:1989-02-07 00:00:00
abstract::The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801483u
更新日期:2009-01-20 00:00:00