当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes.

Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes.

Abstract：

:Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is also involved in anaerobic methane oxidation in reverse of methanogenesis and most probably in the anaerobic oxidation of ethane, propane, and butane. The challenging question is how the unreactive CH3-S thioether bond in methyl-coenzyme M and the even more unreactive C-H bond in methane and the other hydrocarbons are anaerobically cleaved. A key to the answer is the negative redox potential (Eo') of the Ni(II)F-430/Ni(I)F-430 couple below -600 mV and the radical nature of Ni(I)F-430. However, the negative one-electron redox potential is also the Achilles heel of MCR; it makes the nickel enzyme one of the most O2-sensitive enzymes known to date. Even under physiological conditions, the Ni(I) in MCR is oxidized to the Ni(II) or Ni(III) states, e.g., when in the cells the redox potential (E') of the CoM-S-S-CoB/HS-CoM and HS-CoB couple (Eo' = -140 mV) gets too high. Methanogens therefore harbor an enzyme system for the reactivation of inactivated MCR in an ATP-dependent reduction reaction. Purification of active MCR in the Ni(I) oxidation state is very challenging and has been achieved in only a few laboratories. This perspective reviews the function, structure, and properties of MCR, what is known and not known about the catalytic mechanism, how the inactive enzyme is reactivated, and what remains to be discovered.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Thauer RK

doi

10.1021/acs.biochem.9b00164

subject

Has Abstract

pub_date

2019-12-31 00:00:00

pages

5198-5220

issue

52

eissn

0006-2960

issn

1520-4995

journal_volume

58

pub_type

杂志文章,评审

相关文献

BIOCHEMISTRY文献大全
  • Crystal structures of the recombinant kringle 1 domain of human plasminogen in complexes with the ligands epsilon-aminocaproic acid and trans-4-(aminomethyl)cyclohexane-1-carboxylic Acid.

    abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9521351

    authors： Mathews II,Vanderhoff-Hanaver P,Castellino FJ,Tulinsky A

    更新日期：1996-02-27 00:00:00

  • Mapping the bimolecular interface of the parathyroid hormone (PTH)-PTH1 receptor complex: spatial proximity between Lys(27) (of the hormone principal binding domain) and leu(261) (of the first extracellular loop) of the human PTH1 receptor.

    abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000195n

    authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

    更新日期：2000-07-18 00:00:00

  • Molecular organization in striated domains induced by transmembrane alpha-helical peptides in dipalmitoyl phosphatidylcholine bilayers.

    abstract：:Transmembrane (TM) alpha-helical peptides with neutral flanking residues such as tryptophan form highly ordered striated domains when incorporated in gel-state 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers and inspected by atomic force microscopy (AFM) (1). In this study, we analyze the molecular organiz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048047a

    authors： Sparr E,Ganchev DN,Snel MM,Ridder AN,Kroon-Batenburg LM,Chupin V,Rijkers DT,Killian JA,de Kruijff B

    更新日期：2005-01-11 00:00:00

  • Arginine-42 and threonine-45 are required for FAD incorporation and catalytic activity in human monoamine oxidase B.

    abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9806910

    authors： Kirksey TJ,Kwan SW,Abell CW

    更新日期：1998-09-01 00:00:00

  • Heterodimerization of the yeast homeodomain transcriptional regulators alpha 2 and a1 induces an interfacial helix in alpha 2.

    abstract：:The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00197a033

    authors： Phillips CL,Stark MR,Johnson AD,Dahlquist FW

    更新日期：1994-08-09 00:00:00

  • Penicillanic acid sulfone: an unexpected isotope effect in the interaction of 6 alpha- and 6 beta-monodeuterio and of 6,6-dideuterio derivatives with RTEM beta-lactamase from Escherichia coli.

    abstract：:Penicillanic acid sulfone (1) is both a substrate and an inactivator of the RTEM beta-lactamase. About 7000 hydrolytic events occur before enzyme inactivation. The 6,6-dideuterio sulfone shows a 3-fold acceleration of both the hydrolysis reaction and the enzyme inactivation. The kinetic and spectroscopic results are n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a003

    authors： Brenner DG,Knowles JR

    更新日期：1981-06-23 00:00:00

  • Replacement of a conserved proline and the alkaline conformational change in iso-2-cytochrome c.

    abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00451a043

    authors： Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

    更新日期：1989-12-12 00:00:00

  • Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes.

    abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00161a024

    authors： Buser CA,Diner BA,Brudvig GW

    更新日期：1992-11-24 00:00:00

  • Evidence for the kinetic partitioning of polymerase activity on G-quadruplex DNA.

    abstract：:We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00060

    authors： Eddy S,Maddukuri L,Ketkar A,Zafar MK,Henninger EE,Pursell ZF,Eoff RL

    更新日期：2015-05-26 00:00:00

  • Inhibition of peptidyl-prolyl cis/trans isomerase activity by substrate analog structures: thioxo tetrapeptide-4-nitroanilides.

    abstract：:The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00040a012

    authors： Schutkowski M,Wöllner S,Fischer G

    更新日期：1995-10-10 00:00:00

  • Indoleglycerol phosphate synthase-phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains.

    abstract：:Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00016a013

    authors： Eberhard M,Tsai-Pflugfelder M,Bolewska K,Hommel U,Kirschner K

    更新日期：1995-04-25 00:00:00

  • HlyC, the internal protein acyltransferase that activates hemolysin toxin: the role of conserved tyrosine and arginine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis.

    abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990138y

    authors： Trent MS,Worsham LM,Ernst-Fonberg ML

    更新日期：1999-07-06 00:00:00

  • Effect of alpha-methylation on inactivation of monoamine oxidase by N-cyclopropylbenzylamine.

    abstract：:Monoamine oxidase (MAO) was shown previously [Silverman, R. B., & Hoffman, S. J. (1980) J. Am. Chem. Soc. 102, 7126-7128] to catalyze the oxidation of N-cyclopropylbenzylamine (N-CBA) at two sites on the molecule. Oxidation at the benzyl methylene gave benzaldehyde and cyclopropylamine; oxidation of the cyclopropyl gr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00317a019

    authors： Silverman RB

    更新日期：1984-10-23 00:00:00

  • Inhibition of in vitro transcription by a triplex-forming oligonucleotide targeted to human c-myc P2 promoter.

    abstract：:Triplex-forming oligonucleotides (TFOs) have been shown to bind in a sequence-specific manner to polypurine/polypyrimidine sequences in several human gene promoters, including the c-myc P1 promoter. TFOs have been shown to inhibit transcription in vitro and the expression of target genes in cell culture. The human c-m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00025a023

    authors： Kim HG,Miller DM

    更新日期：1995-06-27 00:00:00

  • 2'-O-methyl-RNA hairpins generate loop-loop complexes and selectively inhibit HIV-1 Tat-mediated transcription.

    abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025974d

    authors： Darfeuille F,Arzumanov A,Gait MJ,Di Primo C,Toulmé JJ

    更新日期：2002-10-08 00:00:00

  • Evidence that the FX domain in photosystem I interacts with the subunit PsaC: site-directed changes in PsaB destabilize the subunit interaction in Chlamydomonas reinhardtii.

    abstract：:The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00019a010

    authors： Rodday SM,Webber AN,Bingham SE,Biggins J

    更新日期：1995-05-16 00:00:00

  • Catalytically active cross-species heterodimers of thymidylate synthase.

    abstract：:Thymidylate synthase (TS) is a highly conserved homodimeric enzyme with two active sites, each of which contains amino acid residues from both subunits. We show that the conservation at the subunit interface between Escherichia coli TS and Lactobacillus casei TS is sufficient to permit the formation of a cross-species...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00090a002

    authors： Greene PJ,Maley F,Pedersen-Lane J,Santi DV

    更新日期：1993-10-05 00:00:00

  • Phenylalanine hydroxylase: absolute configuration and source of oxygen of the 4a-hydroxytetrahydropterin species.

    abstract：:The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00333a022

    authors： Dix TA,Bollag GE,Domanico PL,Benkovic SJ

    更新日期：1985-06-04 00:00:00

  • A novel method for determining rate constants for dehydration of aldehyde hydrates.

    abstract：:A method has been developed for calculating rate constants for dehydration of aldehydes that induce ATPase reactions by kinases and where 18O is transferred from the aldehyde or its hydrate to inorganic phosphate during the reaction. The method involves measurement of the fraction of 18O in phosphate by 31P NMR after ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00317a011

    authors： Rendina AR,Hermes JD,Cleland WW

    更新日期：1984-10-23 00:00:00

  • Redox modulation of cyclic electron flow around photosystem I in C3 plants.

    abstract：:We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061439s

    authors： Breyton C,Nandha B,Johnson GN,Joliot P,Finazzi G

    更新日期：2006-11-14 00:00:00

  • Binding to cisplatin-modified DNA by the Saccharomyces cerevisiae HMGB protein Nhp6A.

    abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012077l

    authors： Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

    更新日期：2002-04-30 00:00:00

  • Coherent dynamics during the primary electron-transfer reaction in membrane-bound reaction centers of Rhodobacter sphaeroides.

    abstract：:The temporal evolution of the near-infrared stimulated emission band of the special pair excited state (P*) in the reaction center of Rhodobacter sphaeroides has been studied in intracytoplasmic membranes of the antenna-deficient RCO1 mutant at 10 K with a resolution of 30 fs. On the 100-fs time scale the emission ban...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00188a002

    authors： Vos MH,Jones MR,Hunter CN,Breton J,Lambry JC,Martin JL

    更新日期：1994-06-07 00:00:00

  • Conformational characteristics of deoxyribonucleic acid-butylamine complexes with C-type circular dichroism spectra. 2. A Raman spectroscopic study.

    abstract：:The derivatives of calf thymus DNA in which n-butylamine is covalently attached as described in the preceding paper in this series [Chen, C. Y., Pheiffer, B. H., Zimmerman, S. B., & Hanlon, S. (1983) Biochemistry (preceding paper in this issue)] were examined by Raman spectroscopy. As previously mentioned, these compl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00289a021

    authors： Fish SR,Chen CY,Thomas GJ Jr,Hanlon S

    更新日期：1983-09-27 00:00:00

  • Lipid binding by fragments of apolipoprotein C-III-1 obtained by thrombin cleavage.

    abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00644a004

    authors： Sparrow JT,Pownall HJ,Hsu FJ,Blumenthal LD,Culwell AR,Gotto AM

    更新日期：1977-12-13 00:00:00

  • 3-Bromotyrosine and 3,5-dibromotyrosine are major products of protein oxidation by eosinophil peroxidase: potential markers for eosinophil-dependent tissue injury in vivo.

    abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982401l

    authors： Wu W,Chen Y,d'Avignon A,Hazen SL

    更新日期：1999-03-23 00:00:00

  • Alamethicin incorporation in lipid bilayers: a thermodynamic study.

    abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00384a015

    authors： Rizzo V,Stankowski S,Schwarz G

    更新日期：1987-05-19 00:00:00

  • Substrate specificity of homogeneous monkeypox virus uracil-DNA glycosylase.

    abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700726a

    authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

    更新日期：2007-10-23 00:00:00

  • Functional interactions of recombinant alpha 2 adrenergic receptor subtypes and G proteins in reconstituted phospholipid vesicles.

    abstract：:The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00227a024

    authors： Kurose H,Regan JW,Caron MG,Lefkowitz RJ

    更新日期：1991-04-02 00:00:00

  • Oxidative inactivation of a charge transfer complex in the medium-chain acyl-CoA dehydrogenase.

    abstract：:The intense charge transfer complex between the enolate of 3-thia-octanoyl-CoA and the oxidized flavin of the medium-chain acyl-CoA dehydrogenase is discharged by the ferricenium ion with irreversible inactivation of the enzyme. Charge transfer complex formation is a necessary, but insufficient, condition for oxidativ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00050a025

    authors： Schaller RA,Thorpe C

    更新日期：1995-12-19 00:00:00

  • Development of indolequinone mechanism-based inhibitors of NAD(P)H:quinone oxidoreductase 1 (NQO1): NQO1 inhibition and growth inhibitory activity in human pancreatic MIA PaCa-2 cancer cells.

    abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700008y

    authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

    更新日期：2007-05-22 00:00:00