Abstract：
:A method has been developed for calculating rate constants for dehydration of aldehydes that induce ATPase reactions by kinases and where 18O is transferred from the aldehyde or its hydrate to inorganic phosphate during the reaction. The method involves measurement of the fraction of 18O in phosphate by 31P NMR after the ATPase reaction has proceeded for several minutes with zero-order kinetics. The reaction is started by addition of the aldehyde in a small volume of H2 18O, and the speed of washout of 18O by reversible dehydration relative to the rate of the ATPase reaction allows calculation of the rate constants if the hydration equilibrium constant is known from the proton NMR spectrum of the aldehyde. Dehydration rate constants (s-1 at pH 8-8.5, 0.1 M buffer, 25 degrees C) for the following aldehydes (all over 95% hydrated) and kinases used are as follows: D-glyceraldehyde with glycerokinase, 0.03; 2,5-anhydro-D-mannose 6-phosphate with fructose-6-phosphate kinase, 0.025; 2,5-anhydro-D-mannose or 2,5-anhydro-D-talose with fructokinase, 0.029 and 0.017, respectively; D-gluco-hexodialdose with hexokinase, 0.068. With betaine aldehyde and choline kinase or glyoxylate and pyruvate kinase, no 18O was transferred to phosphate during the ATPase reactions. However, the dehydration rate constant for glyoxylate (0.007 s-1 at pH 7 extrapolated to zero buffer concentration and up to 0.11 s-1 at pH 9.0 with 0.3 M buffer) was determined by extrapolating the initial rate of reduction of the free aldehyde catalyzed by lactate dehydrogenase to infinite enzyme levels.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rendina AR,Hermes JD,Cleland WWdoi
10.1021/bi00317a011subject
Has Abstractpub_date
1984-10-23 00:00:00pages
5148-56issue
22eissn
0006-2960issn
1520-4995journal_volume
23pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Studying the evolution of catalytically promiscuous enzymes like those from the N-succinylamino acid racemase/ o-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal mechanisms by which new functions evolve. Some enzymes in this subfamily have only OSBS activity, while others catalyze OSBS and NSAR reactions. We...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00088
更新日期：2018-07-03 00:00:00
abstract：:The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri , providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is composed of a basal body, external needle, and regulatory tip comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400755f
更新日期：2013-12-10 00:00:00
abstract：:Several basic proteins, including the ubiquitous HU proteins, serve histone-like functions in prokaryotes. Significant sequence conservation exists between HU homologues; yet binding sites varying from 9 to 37 bp have been reported. TF1, an HU homologue with a 37 bp binding site that is encoded by the Bacillus subtili...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi016095e
更新日期：2002-06-18 00:00:00
abstract：:Genetic knockout of the BtR4 gene encoding the Heliothis virescens cadherin-like protein (HevCaLP) is linked to resistance against Cry1Ac toxin from Bacillus thuringiensis. However, the functional Cry1Ac receptor role of this protein has not been established. We previously proposed HevCaLP as a shared binding site for...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0606703
更新日期：2006-08-15 00:00:00
abstract：:Early studies involving purine salvage in Salmonella typhimurium resulted in the isolation and identification of a mutant strain possessing a genetically modified hypoxanthine phosphoribosyl-transferase (HPRT) with enhanced substrate specificity for guanine [Benson, C. E., and Gots, J. S. (1975) J. Bacteriol. 121, 77-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9720179
更新日期：1998-03-10 00:00:00
abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0524215
更新日期：2006-03-21 00:00:00
abstract：:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00222a012
更新日期：1991-02-26 00:00:00
abstract：:Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050030k
更新日期：2005-07-19 00:00:00
abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00227a006
更新日期：1991-04-02 00:00:00
abstract：:It has been shown that in bovine heart submitochondrial particles, antimycin and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) inhibit the oxidation of NADH, succinate, and reduced ubiquinone incompletely, the uninhibited rate being about 20-40 nmol of substrate oxidized min-1 (mg of protein)-1. By contrast, rotenone, cy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00268a045
更新日期：1982-12-07 00:00:00
abstract：:The cell-surface component (alpha) which binds monomeric immunoglobulin E with high affinity is associated with a second polypeptide (beta) in the plasma membrane. The latter component tends to dissociate during purification of the alpha chain from detergent extracts of cells, even at neutral pHs and physiological ion...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00269a047
更新日期：1982-12-21 00:00:00
abstract：:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034426c
更新日期：2003-07-29 00:00:00
abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00685a027
更新日期：1975-07-15 00:00:00
abstract：:The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0006971
更新日期：2000-12-05 00:00:00
abstract：:Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00265a009
更新日期：1982-10-26 00:00:00
abstract：:Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049211x
更新日期：2004-07-27 00:00:00
abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0480887
更新日期：2004-12-21 00:00:00
abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900811p
更新日期：2009-08-25 00:00:00
abstract：:The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051316s
更新日期：2005-11-08 00:00:00
abstract：:We have used diethyl pyrocarbonate (DEP), which carbethoxylates adenine bases, and dimethyl sulfate (DMS), which methylates guanine residues and single-stranded cytosines, to probe bases in open complexes between RNA polymerase and the lac UV5 promoter in vitro. We compared the kinetics of reactivity between bases in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00436a040
更新日期：1989-05-16 00:00:00
abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00600a014
更新日期：1978-04-04 00:00:00
abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035348w
更新日期：2003-12-16 00:00:00
abstract：:The expression of the transforming gene product of Rous sarcoma virus (pp60v-src) in Saccharomyces cerevisiae has recently been reported (Kornbluth et al., 1987; Brugge et al., 1987). To carry out biochemical and structural studies of this enzyme, a facile purification was developed. The purification was accomplished ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00449a013
更新日期：1989-11-14 00:00:00
abstract：:The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00528
更新日期：2019-09-10 00:00:00
abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0355645
更新日期：2004-01-13 00:00:00
abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00238a013
更新日期：1991-06-18 00:00:00
abstract：:Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900946r
更新日期：2009-11-24 00:00:00
abstract：:The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00287a025
更新日期：1983-08-30 00:00:00
abstract：:Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0502339
更新日期：2005-08-16 00:00:00
abstract：:Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00265a007
更新日期：1982-10-26 00:00:00
