Thermodynamic stability of RNA structures formed by CNG trinucleotide repeats. Implication for prediction of RNA structure.

abstract：:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...

journal_title：Biochemistry

authors： Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

更新日期：2001-11-27 00:00:00

abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...

journal_title：Biochemistry

authors： Shin A,Lee E,Jeon D,Park YG,Bang JK,Park YS,Shin SY,Kim Y

更新日期：2015-06-30 00:00:00

abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

journal_title：Biochemistry

authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

更新日期：2000-02-22 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a plasmid-encoded, type II enzyme. Four monomers (78 amino acids long) assemble into a homotetramer possessing 222 symmetry. In previous studies, a tandem array of four R67 DHFR gene copies was fused in frame to generate a functional monomer named Quad1. This protein possessed the...

journal_title：Biochemistry

authors： Feng J,Grubbs J,Dave A,Goswami S,Horner CG,Howell EE

更新日期：2010-08-31 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The reaction of cytochrome c oxidase with hydrogen peroxide has been of great value in generating and characterizing oxygenated species of the enzyme that are identical or similar to those formed during turnover of the enzyme with dioxygen. Most previous studies have utilized relatively low peroxide concentrations (mi...

journal_title：Biochemistry

authors： Zaslavsky D,Smirnova IA,Brzezinski P,Shinzawa-Itoh K,Yoshikawa S,Gennis RB

更新日期：1999-11-30 00:00:00

abstract：:Apolipoprotein (apo) B, the major protein component of the atherogenic low-density lipoprotein (LDL), has a pentapartite structure, NH2-betaalpha1-beta1-alpha2-beta2-alpha3-COOH, the beta domains containing multiple amphipathic beta strands and the alpha domains containing multiple amphipathic alpha helixes. We recent...

journal_title：Biochemistry

authors： Dashti N,Gandhi M,Liu X,Lin X,Segrest JP

更新日期：2002-06-04 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00

abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...

journal_title：Biochemistry

authors： Fann YC,Ahmed I,Blackburn NJ,Boswell JS,Verkhovskaya ML,Hoffman BM,Wikström M

更新日期：1995-08-15 00:00:00

abstract：:TCDD (dioxin) induces a rapid inflammatory response from 3T3-L1 adipocytes as judged by prominent induction of the mRNA expression of prostaglandin-endperoxide synthase 2 (Cox-2) along with other inflammation markers within 1 h. This action of TCDD is clearly antagonized by cell pretreatment with AACOCF3 (an inhibitor...

journal_title：Biochemistry

authors： Li W,Matsumura F

更新日期：2008-12-30 00:00:00

abstract：:In the ras gene superfamily, codon 12 (-TGGTG-) of the K-ras gene is the most frequently mutated codon in human cancers. Recently, we have found that bulky chemical carcinogens preferentially form DNA adducts at codons 12 and 14 (-CGTAG-) in the K-ras gene in normal human bronchial epithelial (NHBE) cells. Furthermore...

journal_title：Biochemistry

authors： Hu W,Feng Z,Tang MS

更新日期：2003-08-26 00:00:00

abstract：:The P-glycoprotein multidrug transporter functions as an ATP-driven efflux pump for a large number of structurally unrelated hydrophobic compounds. Substrates are believed to gain access to the transporter after partitioning into the membrane, rather than from the extracellular aqueous phase. The binding of drug subst...

journal_title：Biochemistry

authors： Romsicki Y,Sharom FJ

更新日期：1999-05-25 00:00:00

abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

journal_title：Biochemistry

authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

更新日期：1998-12-15 00:00:00

abstract：:X-ray absorption spectroscopy at the Zn K-edge indicates that the active site of the marine diatom Thalassiosira weissflogii carbonic anhydrase is strikingly similar to that of mammalian alpha-carbonic anhydrase enzymes. The zinc has three histidine ligands and a single water at 1.98 A. This is quite different from th...

journal_title：Biochemistry

authors： Cox EH,McLendon GL,Morel FM,Lane TW,Prince RC,Pickering IJ,George GN

更新日期：2000-10-10 00:00:00

abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

journal_title：Biochemistry

authors： Büning H,Altman J,Beck W,Zorbas H

更新日期：1997-09-23 00:00:00

abstract：:Tetraheme cytochromes involved in photosynthetic electron transport have previously been described associated with the reaction centers of purple photosynthetic bacteria; however, similar heme proteins have not until now been characterized in the phylogenetically distinct green sulfur bacteria. In this paper we descri...

journal_title：Biochemistry

authors： Albouy D,Sturgis JN,Feiler U,Nitschke W,Robert B

更新日期：1997-02-18 00:00:00

abstract：:Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate...

journal_title：Biochemistry

authors： Oláh J,Szénási T,Szabó A,Kovács K,Lőw P,Štifanić M,Orosz F

更新日期：2017-02-21 00:00:00

abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...

journal_title：Biochemistry

authors： Ahmed SA,McPhie P,Smith LA

更新日期：2003-11-04 00:00:00

abstract：:Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...

journal_title：Biochemistry

authors： Zheng Y,Shopes B,Holowka D,Baird B

更新日期：1992-08-25 00:00:00

abstract：:The laminin alpha3 chain is mainly expressed at the skin, and its C-terminal G domain has a critical role in multiple biological functions. We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 hu...

journal_title：Biochemistry

authors： Urushibata S,Katagiri F,Takaki S,Yamada Y,Fujimori C,Hozumi K,Kikkawa Y,Kadoya Y,Nomizu M

更新日期：2009-11-10 00:00:00

abstract：:The metabolism of the anticonvulsant mephenytoin is subject to a genetic polymorphism. In 2-5% of Caucasians and 18-23% of Japanese subjects a specific cytochrome P-450 isozyme, P-450 meph, is functionally deficient or missing. We have accumulated evidence that autoimmune antibodies observed in sera of patients with t...

journal_title：Biochemistry

authors： Meier UT,Meyer UA

更新日期：1987-12-15 00:00:00

abstract：:Human DNA polymerase kappa (pol kappa) has a sequence significantly homologous with that of Escherichia coli DNA polymerase IV (pol IV). We used a truncated form of human pol kappa (pol kappaDeltaC) and full-length pol IV to explore the miscoding properties of these enzymes. Oligodeoxynucleotides, modified site-specif...

journal_title：Biochemistry

authors： Suzuki N,Ohashi E,Hayashi K,Ohmori H,Grollman AP,Shibutani S

更新日期：2001-12-18 00:00:00

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:Notwithstanding the relevance of their biological function, slow motions in proteins, beyond the microsecond range, are still poorly understood and often elusive. We propose that acrylamide quenching of Trp phosphorescence of deeply buried residues, when extended over the entire accessible range of lifetime measuremen...

journal_title：Biochemistry

authors： Strambini GB,Gonnelli M

更新日期：2009-08-11 00:00:00

abstract：:The three-dimensional structure of the duplex formed by the association of the unnatural oligonucleotide alpha-d(TCTAAACTC) covalently linked to an acridine derivative (m5Acr) with its natural and parallel complementary sequence beta-d(AGATTTGAG) was investigated by nuclear magnetic resonance spectroscopy and constrai...

journal_title：Biochemistry

authors： Guesnet JL,Vovelle F,Thuong NT,Lancelot G

更新日期：1990-05-22 00:00:00

abstract：:The removal of cylclobutane pyrimidine dimers from cellular DNA occurs preferentially in actively transcribed genes of cells subjected to ultraviolet radiation. In contrast, reports concerning the transcription-dependent repair of N-methylpurines formed in cellular DNA following exposure to methylating agents are quit...

journal_title：Biochemistry

authors： Wang W,Sitaram A,Scicchitano DA

更新日期：1995-02-07 00:00:00

abstract：:Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...

journal_title：Biochemistry

authors： Peng D,Satterlee JD,Ma LH,Dallas JL,Smith KM,Zhang X,Sato M,La Mar GN

更新日期：2011-10-18 00:00:00

abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...

journal_title：Biochemistry

authors： Burger RM,Horwitz SB,Peisach J

更新日期：1985-07-02 00:00:00

abstract：:The structure of the anti-inflammatory drug diclofenac bound in the active site of rabbit microsomal cytochrome P450 2C5/3LVdH was determined by X-ray crystallography to 2.1 A resolution. P450 2C5/3LVdH and the related enzyme 2C5dH catalyze the 4'-hydroxylation of diclofenac with apparent K(m) values of 80 and 57 micr...

journal_title：Biochemistry

authors： Wester MR,Johnson EF,Marques-Soares C,Dijols S,Dansette PM,Mansuy D,Stout CD

更新日期：2003-08-12 00:00:00

abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

journal_title：Biochemistry

authors： Ding D,Gryaznov SM,Wilson WD

更新日期：1998-09-01 00:00:00