Structure of mammalian cytochrome P450 2C5 complexed with diclofenac at 2.1 A resolution: evidence for an induced fit model of substrate binding.

abstract：:Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...

journal_title：Biochemistry

authors： Perez-Ramirez B,Shearwin KE,Timasheff SN

更新日期：1994-05-24 00:00:00

abstract：:The deposition of amyloid-β (Aβ) plaques and tau-based neurofibrillary tangles is a neuropathological feature of Alzheimer's disease (AD). While studies have shown that the Aβ and tau interaction results in elevated AD pathology, the molecular linkage and mechanism of interaction of Aβ and tau are unclear. A recent st...

journal_title：Biochemistry

authors： Tripathi T,Khan H

更新日期：2020-02-04 00:00:00

abstract：:Human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2, respectively) exhibit extensive genetic variations. It was postulated that much of this genetic variability stems from the low fidelity of the reverse transcription step. Both HIV reverse transcriptases (RTs) were shown to be particularly error-prone during...

journal_title：Biochemistry

authors： Bakhanashvili M,Hizi A

更新日期：1992-10-06 00:00:00

abstract：:Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To d...

journal_title：Biochemistry

authors： Finer-Moore JS,Maley GF,Maley F,Montfort WR,Stroud RM

更新日期：1994-12-27 00:00:00

abstract：:Incubation of bovine plasma amine oxidase (BPAO) with benzylamine and various p-substituted analogues results in a time-dependent inactivation that is attributable to buildup of the H(2)O(2)-turnover product on the basis of protection afforded by coincubation with catalase. The mechanism of inactivation is distinct fr...

journal_title：Biochemistry

authors： Lee Y,Shepard E,Smith J,Dooley DM,Sayre LM

更新日期：2001-01-23 00:00:00

abstract：:Chemical modification of Torpedo californica acetylcholinesterase by various sulfhydryl reagents results in its conversion to one of two principal states. One of these states, viz., that produced by disulfides and alkylating agents, is stable. The second state, produced by mercury derivatives, is metastable. At room t...

journal_title：Biochemistry

authors： Kreimer DI,Dolginova EA,Raves M,Sussman JL,Silman I,Weiner L

更新日期：1994-12-06 00:00:00

abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...

journal_title：Biochemistry

authors： Nagpal A,Valley MP,Fitzpatrick PF,Orville AM

更新日期：2006-01-31 00:00:00

abstract：:The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...

journal_title：Biochemistry

authors： Voelker DR,Kennedy EP

更新日期：1982-05-25 00:00:00

abstract：:The effect of Ca2+ binding on the circular dichroism (CD) and 270-MHz proton nuclear magnetic resonance (NMR) spectra of brain-specific S-100b calcium binding protein has been examined at two pH values, 8.5 and 7.5. At pH 8.5, S-100b protein binds two Ca2+ per monomer with Kd values of 6 x 10(-5) and 2 x 10(-4) M, whe...

journal_title：Biochemistry

authors： Mani RS,Shelling JG,Sykes BD,Kay CM

更新日期：1983-03-29 00:00:00

abstract：:The mechanism of wild-type and R37A mutant Pseudomonas dacunhae aspartate beta-decarboxylase (ABDC) was studied by rapid-scanning stopped-flow spectrophotometry. Mixing wild-type ABDC with 50 mM disodium l-Asp resulted in the formation of a 325 nm absorption peak within the dead time of the stopped-flow instrument, li...

journal_title：Biochemistry

authors： Phillips RS,Lima S,Khristoforov R,Sudararaju B

更新日期：2010-06-22 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:The structure of a mutant Escherichia coli thioredoxin with a glutamic acid substituted for a conserved lysine at position 36 adjacent to the active site has been solved using molecular replacement and refined at 2.0-A resolution to a crystallographic residual of 19.9%. The mutant was crystallized in an orthorhombic s...

journal_title：Biochemistry

authors： Nikkola M,Gleason FK,Fuchs JA,Eklund H

更新日期：1993-05-18 00:00:00

abstract：:The thermotropic behavior of purified human complement protein C9 was investigated by high-sensitivity differential scanning calorimetry. When dissolved in physiological buffers (pH 7.2, 150 mM NaCl), C9 underwent three endothermic transitions with transition temperatures (Tm) centered at about 32, 48, and 53 degrees ...

journal_title：Biochemistry

authors： Lohner K,Esser AF

更新日期：1991-07-02 00:00:00

abstract：:Single-molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks "internally" labeled in the duplex r...

journal_title：Biochemistry

authors： Lee W,Jose D,Phelps C,Marcus AH,von Hippel PH

更新日期：2013-05-07 00:00:00

abstract：:A compound tentatively identified as O2-methyl-5-carboxymethyluridine (cm5Um) was recently isolated in this laboratory from bulk yeast transfer RNA (Gray, M. W. (1975), Can, J. Biochem. 53, 735-746). Alkaline hydrolysis of yeast tRNA releases this nucleoside as part of an alkali-stable dinucleotide, cm5Um-Ap, from whi...

journal_title：Biochemistry

authors： Gray MW

更新日期：1976-07-13 00:00:00

abstract：:The role of the "C-terminal tail" segment of long neurotoxins has been investigated. The C-terminal four to five residues of alpha-bungarotoxin and Laticauda colubrina b have been cleaved off by carboxypeptidase P. The effect of such deletion on the toxin conformation has been monitored in proton nuclear magnetic reso...

journal_title：Biochemistry

authors： Endo T,Oya M,Tamiya N,Hayashi K

更新日期：1987-07-14 00:00:00

abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

journal_title：Biochemistry

authors： Laughrea M,Jetté L

更新日期：1996-02-06 00:00:00

abstract：:In the absence of ligands, dihydrofolate reductase from Escherichia coli exists in at least two interconvertible conformations, only one of which binds NADPH with high affinity. This equilibrium is pH dependent, involving an ionizable group of the enzyme (pK approximately 5.5), and the proportion of the NADPH-binding ...

journal_title：Biochemistry

authors： Dunn SM,Lanigan TM,Howell EE

更新日期：1990-09-18 00:00:00

abstract：:The 24-kDa subunit of mitochondrial NADH-ubiquinone reductase (complex I) is an iron-sulfur protein that is present in the flavoprotein or NADH dehydrogenase II subcomplex. It is a nuclear gene product and is imported into the organelle. A group of human patients with mitochondrial myopathy have been shown to have red...

journal_title：Biochemistry

authors： Pilkington SJ,Walker JE

更新日期：1989-04-18 00:00:00

abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

journal_title：Biochemistry

authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

更新日期：1994-11-01 00:00:00

abstract：:Trypanosoma and Leishmania, pathogens responsible for diseases such as African sleeping sickness, Chagas' heart disease, or Oriental sore, are two of the very few genera that do not use the ubiquitous glutathione/glutathione reductase system to keep a stable cellular redox balance. Instead, they rely on trypanothione ...

journal_title：Biochemistry

authors： Stoll VS,Simpson SJ,Krauth-Siegel RL,Walsh CT,Pai EF

更新日期：1997-05-27 00:00:00

abstract：:We have used [(1)H-(15)N]-HSQC NMR to investigate the structural changes that occur in both serpin and proteinase in forming the kinetically trapped covalent protein-protein complex that is the basis for serpin inhibition of serine proteinases. By alternately using (15)N-alanine specifically-labeled alpha(1)-proteinas...

journal_title：Biochemistry

authors： Peterson FC,Gettins PG

更新日期：2001-05-29 00:00:00

abstract：:The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...

journal_title：Biochemistry

authors： Görisch H,Goss DJ,Parkhurst LJ

更新日期：1976-12-28 00:00:00

abstract：:The effect of Ca2+ on the interaction of bovine cardiac myosin subfragment 1 (S-1) with actin regulated by cardiac troponin-tropomyosin was evaluated. The ratios of actin to troponin and to tropomyosin were adjusted to optimize the Ca2+-dependent regulation of the steady-state actin-activated magnesium adenosinetripho...

journal_title：Biochemistry

authors： Tobacman LS,Adelstein RS

更新日期：1986-02-25 00:00:00

abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...

journal_title：Biochemistry

authors： Caplow M,Shanks J

更新日期：1998-09-15 00:00:00

abstract：:The reactions of Rhodopseudomonas viridis cytochrome c2 and horse cytochrome c with Rps. viridis photosynthetic reaction centers were studied by using both single- and double-flash excitation. Single-flash excitation of the reaction centers resulted in rapid photooxidation of cytochrome c-556 in the cytochrome subunit...

journal_title：Biochemistry

authors： Knaff DB,Willie A,Long JE,Kriauciunas A,Durham B,Millett F

更新日期：1991-02-05 00:00:00

abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

journal_title：Biochemistry

authors： Arndt JW,Chai Q,Christian T,Stevens RC

更新日期：2006-03-14 00:00:00

abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

journal_title：Biochemistry

authors： Yano Y,Matsuzaki K

更新日期：2002-10-15 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...

journal_title：Biochemistry

authors： Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

更新日期：2015-09-29 00:00:00

abstract：:Neuronal PAS protein 2 (NPAS2), a heme-binding transcriptional regulatory factor, is involved in circadian rhythms. Period homologue (Per) is another important transcriptional regulatory factor that binds to cryptochrome (Cry). The resultant Per/Cry heterodimer interacts with the NPAS2/BMAL1 heterodimer to inhibit the...

journal_title：Biochemistry

authors： Kitanishi K,Igarashi J,Hayasaka K,Hikage N,Saiful I,Yamauchi S,Uchida T,Ishimori K,Shimizu T

更新日期：2008-06-10 00:00:00