Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity.

abstract：:The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Win...

journal_title：Biochemistry

authors： Lu M,Guo Q,Kallenbach NR,Sheardy RD

更新日期：1992-05-19 00:00:00

abstract：:In Gram-negative bacteria, the first step of lipid A biosynthesis is catalyzed by UDP-N-acetylglucosamine acyltransferase (LpxA) through the transfer of a R-3-hydroxyacyl chain from the acyl carrier protein (ACP) to the 3-hydroxyl group of UDP-GlcNAc. Previous studies suggest that LpxA is a critical determinant of the...

journal_title：Biochemistry

authors： Smith EW,Zhang X,Behzadi C,Andrews LD,Cohen F,Chen Y

更新日期：2015-09-29 00:00:00

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...

journal_title：Biochemistry

authors： Techtmann SM,Ghirlando R,Kao S,Strebel K,Maynard EL

更新日期：2012-03-13 00:00:00

abstract：:The solution nuclear magnetic resonance (NMR) structures and backbone (15)N dynamics of the specialized acyl carrier protein (ACP), RpAcpXL, from Rhodopseudomonas palustris, in both the apo form and holo form modified by covalent attachment of 4'-phosphopantetheine at S37, are virtually identical, monomeric, and corre...

journal_title：Biochemistry

authors： Ramelot TA,Rossi P,Forouhar F,Lee HW,Yang Y,Ni S,Unser S,Lew S,Seetharaman J,Xiao R,Acton TB,Everett JK,Prestegard JH,Hunt JF,Montelione GT,Kennedy MA

更新日期：2012-09-18 00:00:00

abstract：:Sufiredoxins (Srx) repair the inactivated forms of typical two-Cys peroxiredoxins (Prx) implicated in hydrogen peroxide-mediated cell signaling. The reduction of the cysteine sulfinic acid moiety within the active site of the Prx by Srx involves novel sulfur chemistry and the use of ATP and Mg(2+). The 1.65 A crystal ...

journal_title：Biochemistry

authors： Jönsson TJ,Murray MS,Johnson LC,Poole LB,Lowther WT

更新日期：2005-06-21 00:00:00

abstract：:M13 DNA containing 20-30 apurinic/apyrimidinic (AP) sites per intact circular molecule was prepared by growing phage on an ung- dut- Escherichia coli mutant and treating the DNA with uracil N-glycosylase. AP sites obstruct in vitro DNA synthesis catalyzed by E. coli pol I. The position at which termination of synthesi...

journal_title：Biochemistry

authors： Sagher D,Strauss B

更新日期：1983-09-13 00:00:00

abstract：:Three tyrosine-to-phenylalanine mutants of ribonuclease A (Y25F, Y92F, and Y97F) are investigated for their enzymatic activities, molecular stabilities, and unfolding/refolding kinetics. These mutants exhibit 80, 90, and 80%, respectively, of the catalytic activity of the wild-type enzyme. Thermal, Gdn.HCl, and pH tra...

journal_title：Biochemistry

authors： Juminaga D,Wedemeyer WJ,Garduño-Júarez R,McDonald MA,Scheraga HA

更新日期：1997-08-19 00:00:00

abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

journal_title：Biochemistry

authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

更新日期：2004-01-13 00:00:00

abstract：:Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...

journal_title：Biochemistry

authors： Varadarajan S,Shah D,Dande P,Settles S,Chen FX,Fronza G,Gold B

更新日期：2003-12-09 00:00:00

abstract：:The sucrose (CscB) permease belongs to the oligosaccharide:H(+) symporter family of the Major Facilitator Superfamily and is homologous to the lactose permease from Escherichia coli. Sucrose transport in cells expressing sucrose permease is completely inhibited by N-ethylmaleimide (NEM), suggesting that one or more of...

journal_title：Biochemistry

authors： Sahin-Tóth M,Frillingos S,Lawrence MC,Kaback HR

更新日期：2000-05-23 00:00:00

abstract：:The effect on overall alpha-helix content of substituting proline for alanine has been determined at 5 positions (1, 2, 4, 5, and 13) of a 13-residue peptide related in sequence to residues 1-13 of ribonuclease A. The helix content falls off rapidly as proline is moved inward, and the proline residue effectively trunc...

journal_title：Biochemistry

authors： Strehlow KG,Robertson AD,Baldwin RL

更新日期：1991-06-11 00:00:00

abstract：:The gamma-phosphoryl groups of two intermediates (M-ATP and M-ADP-P1) in the pathway of MgATP hydrolysis by myosin undergo extensive oxygen exchange with water. Actin activates the overall rate of hydrolysis at a rate-limiting step which follows these exchange reactions. Thus, actin, by decreasing the turnover time of...

journal_title：Biochemistry

authors： Shukla KK,Levy HM

更新日期：1977-01-11 00:00:00

abstract：:Oxidative damage by transition metals bound to proteins may be an important pathogenic mechanism. Ceruloplasmin (Cp) is a Cu-containing plasma protein thought to be involved in oxidative modification of lipoproteins. We have previously shown that Cp increased cell-mediated low-density lipoprotein (LDL) oxidation by a ...

journal_title：Biochemistry

authors： Mukhopadhyay CK,Fox PL

更新日期：1998-10-06 00:00:00

abstract：:The mammalian iron-binding proteins lactoferrin (Lf) and transferrin (Tf) bind iron very tightly, but reversibly. Despite homologous structures and essentially identical iron binding sites, Tf begins to release iron at pH 6.0, whereas Lf retains iron to pH approximately 3.5. This difference in iron retention gives the...

journal_title：Biochemistry

authors： Peterson NA,Arcus VL,Anderson BF,Tweedie JW,Jameson GB,Baker EN

更新日期：2002-12-03 00:00:00

abstract：:The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-i...

journal_title：Biochemistry

authors： Jockel P,Schmid M,Choinowski T,Dimroth P

更新日期：2000-04-18 00:00:00

abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

journal_title：Biochemistry

authors： Roychowdhury S,Rasenick MM

更新日期：1994-08-16 00:00:00

abstract：:The cAMP-dependent protein kinase (cAPK) plays an essential role during differentiation and fruit morphogenesis in Dictyostelium discoideum. The presence of an open reading frame on the gene, pkaC (previously named either Dd PK2 or Dd PK3 by different groups), predicts a 73-kDa polypeptide with 54% similarity to the c...

journal_title：Biochemistry

authors： Anjard C,Etchebehere L,Pinaud S,Véron M,Reymond CD

更新日期：1993-09-21 00:00:00

abstract：:Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated am...

journal_title：Biochemistry

authors： Polverini E,Rangaraj G,Libich DS,Boggs JM,Harauz G

更新日期：2008-01-08 00:00:00

abstract：:Two series of experiments were carried out to characterize (a) peptide fragments of sarcoplasmic reticulum (SR) ATPase, based on proteolysis with different enzymes and distribution of known labels, and (b) specific labeling and functional inactivation patterns, following ATPase derivatization with dicyclohexylcarbodii...

journal_title：Biochemistry

authors： de Ancos JG,Inesi G

更新日期：1988-03-08 00:00:00

abstract：:Intramolecular excimerization of 1,3-di-1-pyrenylpropane [Py(3)Py] was used to assess the fluidity of sarcoplasmic reticulum membranes (SR); on the basis of the spectral data, the probe incorporates completely inside the membrane probably somewhere close to the polar head groups of phospholipid molecules, however not ...

journal_title：Biochemistry

authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

更新日期：1984-09-25 00:00:00

abstract：:Human heart short chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) catalyzes the oxidation of the hydroxyl group of L-3-hydroxyacyl-CoA to a keto group, concomitant with the reduction of NAD+ to NADH, as part of the beta-oxidation pathway. The homodimeric enzyme has been overexpressed in Escherichia coli, purified to h...

journal_title：Biochemistry

authors： Barycki JJ,O'Brien LK,Bratt JM,Zhang R,Sanishvili R,Strauss AW,Banaszak LJ

更新日期：1999-05-04 00:00:00

abstract：:Vc H-NOX (or VCA0720) is an H-NOX (heme-nitric oxide and oxygen binding) protein from facultative aerobic bacterium Vibrio cholerae. It shares significant sequence homology with soluble guanylyl cyclase (sGC), a NO sensor protein commonly found in animals. Similar to sGC, Vc H-NOX binds strongly to NO and CO with affi...

journal_title：Biochemistry

authors： Wu G,Liu W,Berka V,Tsai AL

更新日期：2013-12-31 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...

journal_title：Biochemistry

authors： Rosen CA,Siekierka J,Ennis HL,Cohen PS

更新日期：1984-05-22 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...

journal_title：Biochemistry

authors： Robbins D,Odom OW Jr,Lynch J,Kramer G,Hardesty B,Liou R,Ofengand J

更新日期：1981-09-01 00:00:00

abstract：:Nuclear DNA of the marine chromophytic alga Olisthodiscus luteus was analyzed in this study. Reassociation kinetics analysis has shown that 440-nucleotide DNA fragments from the genome of this alga contain 4% foldback, 58% repetitive, and 34% single-copy sequences. Precise analysis using isolated single-copy DNA revea...

journal_title：Biochemistry

authors： Ersland DR,Cattolico RA

更新日期：1981-11-24 00:00:00

abstract：:The high resolution proton nuclear magnetic resonance (NMR) spectra of the synthetic DNA block polymer d(C15A15)-d(T15G15) were studied in order to more completely understand telestability in DNA, and to provide fundamental NMR data on DNA helices and random coils. Spectra were measured in the spectral region from 0 t...

journal_title：Biochemistry

authors： Early TA,Kearns DR,Burd JF,Larson JE,Wells RD

更新日期：1977-02-08 00:00:00