Structures of Pseudomonas aeruginosa LpxA Reveal the Basis for Its Substrate Selectivity.

abstract：:Protein engineering to alter recognition underlying ligand binding and activity has enormous potential. Here, ligand binding for Escherichia coli phosphoenolpyruvate carboxykinase (PEPCK), which converts oxaloacetate into CO2 and phosphoenolpyruvate as the first committed step in gluconeogenesis, was engineered to acc...

journal_title：Biochemistry

authors： Tang HYH,Shin DS,Hura GL,Yang Y,Hu X,Lightstone FC,McGee MD,Padgett HS,Yannone SM,Tainer JA

更新日期：2018-12-04 00:00:00

abstract：:The interaction of Triton X-100 and other nonionic detergents with a delipidated preparation of the Ca2+ ATPase from sarcoplasmic reticulum has been studied. Binding of radiolabeled Triton X-100 was determined by column chromatography at 6 degrees C, and two classes of binding sites were observed. Below the critical m...

journal_title：Biochemistry

authors： Dean WL,Suárez CP

更新日期：1981-03-31 00:00:00

abstract：:The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

journal_title：Biochemistry

authors： Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

更新日期：1997-03-25 00:00:00

abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

journal_title：Biochemistry

authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

更新日期：2002-12-31 00:00:00

abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...

journal_title：Biochemistry

authors： Oatley P,Stewart AP,Sandford R,Edwardson JM

更新日期：2012-04-03 00:00:00

abstract：:Mass spectrometry was used to identify the sites of covalent attachment of [(14)C]-17alpha-bromoacetamidopropylestradiol ([(14)C]17BAPE(2), an estradiol agonist) to the ligand-binding domain (LBD) of mouse estrogen receptor alpha (ERalpha). A glutathione S-transferase (GST)-LBD chimera protein was overexpressed in Esc...

journal_title：Biochemistry

authors： Mattras H,Aliau S,Richard E,Bonnafous JC,Jouin P,Borgna JL

更新日期：2002-12-31 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00

abstract：:The R2 protein of ribonucleotide reductase catalyzes the dioxygen-dependent one-electron oxidation of Tyr122 at a diiron-carboxylate site. Methane monooxygenase and related hydroxylases catalyze hydrocarbon hydroxylation at diiron sites structurally related to the one in R2. In protein R2, the likely reaction site for...

journal_title：Biochemistry

authors： Logan DT,deMaré F,Persson BO,Slaby A,Sjöberg BM,Nordlund P

更新日期：1998-07-28 00:00:00

abstract：:Initiation of RNA synthesis by Bacillus subtilis RNA polymerase (sigma-43) has been examined at two early promoters of phage phi 29: the A2 promoter, which is a weak promoter, and the G2 promoter, which is a strong promoter. The delta subunit of the polymerase inhibits the rate of initiation at A2, but not G2. In addi...

journal_title：Biochemistry

authors： Dobinson KF,Spiegelman GB

更新日期：1987-12-15 00:00:00

abstract：:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-...

journal_title：Biochemistry

authors： Sears B,Hutton WC,Thompson TE

更新日期：1976-04-20 00:00:00

abstract：:The nitrophorins are a family of proteins that use ferric heme to transport nitric oxide (NO) from the salivary glands of blood-sucking insects to their victims, resulting in vasodilation and reduced blood coagulation. We have refined atomic resolution structures of nitrophorin 4 (NP4) from Rhodnius prolixus complexed...

journal_title：Biochemistry

authors： Roberts SA,Weichsel A,Qiu Y,Shelnutt JA,Walker FA,Montfort WR

更新日期：2001-09-25 00:00:00

abstract：:Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...

journal_title：Biochemistry

authors： Swart GW,Parmeggiani A

更新日期：1989-01-10 00:00:00

abstract：:A mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotope trapping experiments and kinetic isotope effects on V/K for the ST-catalyzed reaction of isotopically labeled CMP-N-acetylneuraminate and N-acetyllactosamine. The isotope trapping experiments confirmed that the kin...

journal_title：Biochemistry

authors： Bruner M,Horenstein BA

更新日期：1998-01-06 00:00:00

abstract：:Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte li...

journal_title：Biochemistry

authors： Romanova LY,Alexandrov IA,Nordan RP,Blagosklonny MV,Mushinski JF

更新日期：1998-04-21 00:00:00

abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...

journal_title：Biochemistry

authors： Agarwal T,Roy S,Kumar S,Chakraborty TK,Maiti S

更新日期：2014-06-17 00:00:00

abstract：:A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...

journal_title：Biochemistry

authors： Miller JS,Burgess RR

更新日期：1978-05-30 00:00:00

abstract：:Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ternary complexes influence ET. We probe this issue through a study of the photocycle...

journal_title：Biochemistry

authors： Page TR,Hoffman BM

更新日期：2015-02-10 00:00:00

abstract：:Purification and potential tachykinin and enkephalin precursor cleaving enzymes from bovine chromaffin granules was undertaken using as substrates the model precursors 35S-(Met)-beta-preprotachykinin [35S-(Met)-beta-PPT] and 35S-(Met)-preproenkephalin [35S-(Met)-PPE]. Purification by concanavalin A-Sepharose, Sephacry...

journal_title：Biochemistry

authors： Krieger TJ,Hook VY

更新日期：1992-05-05 00:00:00

abstract：:During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...

journal_title：Biochemistry

authors： Casy W,Prater AR,Cornish PV

更新日期：2018-04-03 00:00:00

abstract：:CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...

journal_title：Biochemistry

authors： Furuhata Y,Kato Y

更新日期：2021-01-26 00:00:00

abstract：:Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...

journal_title：Biochemistry

authors： Snitko Y,Koduri RS,Han SK,Othman R,Baker SF,Molini BJ,Wilton DC,Gelb MH,Cho W

更新日期：1997-11-25 00:00:00

abstract：:Protein carboxyl methyltransferases (EC 2.1.1.77) that catalyze the transfer of a methyl group from S-adenosylmethionine to L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely, but not universally, distributed in nature. These enzymes can participate in the repair of damaged proteins...

journal_title：Biochemistry

authors： Mudgett MB,Clarke S

更新日期：1993-10-19 00:00:00

abstract：:The influenza M2 proton channel is a major drug target, but unfortunately, the acquisition of resistance mutations greatly reduces the functional life span of a drug in influenza treatment. New M2 inhibitors that inhibit mutant M2 channels otherwise resistant to the early adamantine-based drugs have been reported, but...

journal_title：Biochemistry

authors： Santner P,Martins JMDS,Kampmeyer C,Hartmann-Petersen R,Laursen JS,Stein A,Olsen CA,Arkin IT,Winther JR,Willemoës M,Lindorff-Larsen K

更新日期：2018-10-16 00:00:00

abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...

journal_title：Biochemistry

authors： Robbins D,Odom OW Jr,Lynch J,Kramer G,Hardesty B,Liou R,Ofengand J

更新日期：1981-09-01 00:00:00

abstract：:Clustering of anionic lipids away from zwitterionic ones by cationic antimicrobial agents has recently been established as a mechanism of action of natural small, flexible peptides as well as non-natural synthetic peptide mimics. One of the largest classes of antimicrobial peptides consists of peptides that form catio...

journal_title：Biochemistry

authors： Epand RF,Maloy WL,Ramamoorthy A,Epand RM

更新日期：2010-05-18 00:00:00

abstract：:Parkinson's disease (PD) involves loss of dopaminergic neurons in the substantia nigra and is characterized by intracellular inclusions, Lewy bodies, consisting primarily of aggregated alpha-synuclein. Two substitution mutations (A53T and A30P) in alpha-synuclein gene have been identified in familial early-onset PD. T...

journal_title：Biochemistry

authors： Moussa CE,Wersinger C,Tomita Y,Sidhu A

更新日期：2004-05-11 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic ch...

journal_title：Biochemistry

authors： Singh RK,Vikram P,Makker J,Jabeen T,Sharma S,Dey S,Kaur P,Srinivasan A,Singh TP

更新日期：2003-10-14 00:00:00

abstract：:Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appea...

journal_title：Biochemistry

authors： Silow M,Tan YJ,Fersht AR,Oliveberg M

更新日期：1999-10-05 00:00:00

abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...

journal_title：Biochemistry

authors： Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

更新日期：2006-10-31 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00