当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Effects of paramagnetic shift reagents on the 13C nuclear magnetic resonance spectra of egg phosphatidylcholine enriched with 13C in the N-methyl carbons.

Effects of paramagnetic shift reagents on the 13C nuclear magnetic resonance spectra of egg phosphatidylcholine enriched with 13C in the N-methyl carbons.

Abstract：

:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-methyl carbon resonances is obtained from molecules on the inner and outer faces of the vesicle bilayer. No precipitation of the vesicles is caused by Yb3+ at these concentrations nor is appreciable line broadening observed. Other paramagnetic shift reagents frequently used in proton NMR investigations of phosphatidylcholine vesicles do not give complete separation of the N-methyl 13C signals from the two bilayer surfaces. K3Fe(CN)b,Eu3+, and Pr3+ cause precipitation of the phosphatidylcholine vesicles at concentrations, which give only incomplete resolution of these signals. T1 measurements of the resonances separated by Yb3+ indicate that the choline groups on the inner bilayer surface are less mobile than are the same groups in the outer surface. Gated proton decoupling measurements, which show that the nuclear Overhauser effect is 2.8 +/- 0.1, indicate that the dominant mode of relaxation is dipolar interaction.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Sears B,Hutton WC,Thompson TE

doi

10.1021/bi00653a007

subject

Has Abstract

pub_date

1976-04-20 00:00:00

pages

1635-9

issue

8

eissn

0006-2960

issn

1520-4995

journal_volume

15

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Effects of a distal mutation on active site chemistry.

    abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0518242

    authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

    更新日期：2006-02-07 00:00:00

  • Resonance raman analysis of chromophore structure in the lumi-R photoproduct of phytochrome.

    abstract：:Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is com...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962175k

    authors： Andel F 3rd,Lagarias JC,Mathies RA

    更新日期：1996-12-17 00:00:00

  • Protein kinase C phosphorylation modulates N- and C-terminal regulatory activities of the PITX2 homeodomain protein.

    abstract：:PKC phosphorylation regulates PITX2 DNA binding and transcriptional activity. Mutation of individual PKC sites demonstrates the functional regulation of PITX2 through phosphorylation. Immunoprecipitation of PITX2 and a PITX2 PKC mutant protein reveal specific in vivo phosphorylation by PKC in transfected cells. The tr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048362x

    authors： Espinoza HM,Ganga M,Vadlamudi U,Martin DM,Brooks BP,Semina EV,Murray JC,Amendt BA

    更新日期：2005-03-15 00:00:00

  • Structural Elucidation of Peptide Binding to KLHL-12, a Substrate Specific Adapter Protein in a Cul3-Ring E3 Ligase Complex.

    abstract：:KLHL-12 is a substrate specific adapter protein for a Cul3-Ring ligase complex. It is a member of the Kelch β-propeller domain subclass of Cullin-Ring substrate recognition domains. This E3 ubiquitin ligase complex has many activities, including acting as a negative regulator of the Wnt signaling pathway by mediating ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b01073

    authors： Zhao B,Payne WG,Sai J,Lu Z,Olejniczak ET,Fesik SW

    更新日期：2020-03-03 00:00:00

  • His...Asp catalytic dyad of ribonuclease A: structure and function of the wild-type, D121N, and D121A enzymes.

    abstract：:The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972766q

    authors： Schultz LW,Quirk DJ,Raines RT

    更新日期：1998-06-23 00:00:00

  • Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation.

    abstract：:Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9000062

    authors： Rajala A,Daly RJ,Tanito M,Allen DT,Holt LJ,Lobanova ES,Arshavsky VY,Rajala RV

    更新日期：2009-06-23 00:00:00

  • Binding of calcium to anticoagulant protein S: role of the fourth EGF module.

    abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601151

    authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

    更新日期：2006-09-05 00:00:00

  • Product Release Mechanism Associated with Structural Changes in Monomeric l-Threonine 3-Dehydrogenase.

    abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00832

    authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

    更新日期：2017-10-31 00:00:00

  • Conformations of bombolitins I and III in aqueous solutions: circular dichroism, 1H NMR, and computer simulation studies.

    abstract：:The heptadecapeptides bombolitin I and bombolitin III are two of a series of peptides postulated to be biologically active within a membrane environment. In the preceding paper [Bairaktari, E., Mierke, D.F., Mammi, S., & Peggion, E. (1990) Biochemistry (preceding paper in this issue)] the conformational preferences of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00495a012

    authors： Bairaktari E,Mierke DF,Mammi S,Peggion E

    更新日期：1990-10-30 00:00:00

  • Fluorescence energy transfer between Ca2+ transport ATPase molecules in artificial membranes.

    abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00626a003

    authors： Vanderkooi JM,Ierokomas A,Nakamura H,Martonosi A

    更新日期：1977-04-05 00:00:00

  • A novel epsilon-cleavage within the transmembrane domain of the Alzheimer amyloid precursor protein demonstrates homology with Notch processing.

    abstract：:Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015794o

    authors： Weidemann A,Eggert S,Reinhard FB,Vogel M,Paliga K,Baier G,Masters CL,Beyreuther K,Evin G

    更新日期：2002-02-26 00:00:00

  • Atrazine chlorohydrolase from Pseudomonas sp. strain ADP is a metalloenzyme.

    abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020415s

    authors： Seffernick JL,McTavish H,Osborne JP,de Souza ML,Sadowsky MJ,Wackett LP

    更新日期：2002-12-03 00:00:00

  • Dynamics of activation of lecithin:cholesterol acyltransferase by apolipoprotein A-I.

    abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901242k

    authors： Jones MK,Catte A,Li L,Segrest JP

    更新日期：2009-12-01 00:00:00

  • Improvement by benzoquinones of the quantum yield of photoactivation of photosynthetic oxygen evolution: direct evidence for the two-quantum mechanism.

    abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a032

    authors： Miyao-Tokutomi M,Inoue Y

    更新日期：1992-01-21 00:00:00

  • Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis.

    abstract：:The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9517751

    authors： Kumar RA,Vaze MB,Chandra NR,Vijayan M,Muniyappa K

    更新日期：1996-02-13 00:00:00

  • Modified nucleosides in the first positions of the anticodons of tRNA(Leu)4 and tRNA(Leu)5 from Escherichia coli.

    abstract：:Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981865g

    authors： Horie N,Yamaizumi Z,Kuchino Y,Takai K,Goldman E,Miyazawa T,Nishimura S,Yokoyama S

    更新日期：1999-01-05 00:00:00

  • A rotary mechanism for coenzyme B(12) synthesis by adenosyltransferase.

    abstract：:Adenosyltransferases (ATRs) catalyze the synthesis of the reactive cobalt-carbon bond found in coenzyme B(12) or 5'-deoxyadenosylcobalamin (AdoCbl), which serves as a cofactor for a number of isomerases. The reaction involves a reductive adenosylation of cob(II)alamin in which an electron delivered by a reductase redu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900454s

    authors： Padovani D,Banerjee R

    更新日期：2009-06-16 00:00:00

  • EPR and Mössbauer spectroscopy of intact mitochondria isolated from Yah1p-depleted Saccharomyces cerevisiae.

    abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801047q

    authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

    更新日期：2008-09-16 00:00:00

  • Gangliosides as markers of cortisone-sensitive and cortisone-resistant rabbit thymocytes: characterization of thymus-specific gangliosides and preferential changes of particular gangliosides in the thymus of cortisone-treated rabbits.

    abstract：:Neutral glycosphingolipids and gangliosides in rabbit thymus, spleen, bone marrow, and erythrocyte ghosts were analyzed by conventional chemical and enzymatic procedures and negative ion fast atom bombardment mass spectrometry (FABMS). Thymus gangliosides showed a characteristic composition. Major gangliosides compris...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00352a023

    authors： Iwamori M,Kiguchi K,Kanno J,Kitagawa M,Nagai Y

    更新日期：1986-02-25 00:00:00

  • Clustered negative charges on the lipid membrane surface induce beta-sheet formation of prion protein fragment 106-126.

    abstract：:The conformational conversion of prion protein (PrP) from an alpha-helix-rich normal cellular isoform (PrPC) to a beta-sheet-rich pathogenic isoform (PrPSc) is a key event in the development of prion diseases, and it takes place in caveolae, cavelike invaginations of the plasma membrane. A peptide homologous to residu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700939j

    authors： Miura T,Yoda M,Takaku N,Hirose T,Takeuchi H

    更新日期：2007-10-16 00:00:00

  • Water structural changes involved in the activation process of photoactive yellow protein.

    abstract：:Fourier transform infrared (FTIR) spectroscopy was applied to the blue-light photoreceptor photoactive yellow protein (PYP) to investigate water structural changes possibly involved in the photocycle of PYP. Photointermediates were stabilized at low temperature, and difference IR spectra were obtained between intermed...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000357f

    authors： Kandori H,Iwata T,Hendriks J,Maeda A,Hellingwerf KJ

    更新日期：2000-07-11 00:00:00

  • Systematic comparison of statistical analyses of electronic and vibrational circular dichroism for secondary structure prediction of selected proteins.

    abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00242a012

    authors： Pancoska P,Keiderling TA

    更新日期：1991-07-16 00:00:00

  • Active-site cobalt(II)-substituted horse liver alcohol dehydrogenase: characterization of intermediates in the oxidation and reduction processes as a function of pH.

    abstract：:Substitution of Co(II) for the catalytic site Zn(II) of horse liver alcohol dehydrogenase (LADH) yields an active enzyme derivative, CoIIE, with characteristic Co(II) charge-transfer and d-d electronic transitions that are sensitive to the events which take place during catalysis [Koerber, S. C., MacGibbon, A. K. H., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00377a031

    authors： Sartorius C,Gerber M,Zeppezauer M,Dunn MF

    更新日期：1987-02-10 00:00:00

  • Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity.

    abstract：:UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that operates as a gatekeeper for quality control by preventing transport of improperly folded glycoproteins out of the ER. We report the isolation of two cDNAs encoding human UDP-glucose:glycoprotein glucosyltran...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9916473

    authors： Arnold SM,Fessler LI,Fessler JH,Kaufman RJ

    更新日期：2000-03-07 00:00:00

  • Signal amplification between Gbetagamma release and PI3Kgamma-mediated PI(3,4,5)P3 formation monitored by a fluorescent Gbetagamma biosensor protein and repetitive two component total internal reflection/fluorescence redistribution after photobleaching an

    abstract：:Phosphoinositide 3-kinase gamma (PI3Kgamma) is activated by Gbetagamma release after stimulation of Galpha i -coupled receptors, involving a recruitment of the enzyme to the plasma membrane via interaction of the regulatory subunit p101 or p87 with Gbetagamma. The receptor-mediated release of Gbetagamma was, however, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800596b

    authors： Tannert A,Voigt P,Burgold S,Tannert S,Schaefer M

    更新日期：2008-10-28 00:00:00

  • Structural determinants of cytochrome P450 2B1 specificity: evidence for five substrate recognition sites.

    abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00180a040

    authors： He Y,Luo Z,Klekotka PA,Burnett VL,Halpert JR

    更新日期：1994-04-12 00:00:00

  • Excitation-Wavelength-Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila.

    abstract：:Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01114

    authors： Mix LT,Carroll EC,Morozov D,Pan J,Gordon WR,Philip A,Fuzell J,Kumauchi M,van Stokkum I,Groenhof G,Hoff WD,Larsen DS

    更新日期：2018-03-20 00:00:00

  • Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding.

    abstract：:Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801202a

    authors： Thackray SJ,Bruckmann C,Anderson JL,Campbell LP,Xiao R,Zhao L,Mowat CG,Forouhar F,Tong L,Chapman SK

    更新日期：2008-10-07 00:00:00

  • Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition.

    abstract：:P-Selectin, expressed on activated endothelial cells and platelets, is a high kinetic adhesion receptor involved in leukocyte rolling of the inflammatory response, or in tumor cell binding in the course of metastasis. Thus, P-selectin inhibition is a promising therapeutic target. The anti-inflammatory and anti-metasta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602347g

    authors： Simonis D,Fritzsche J,Alban S,Bendas G

    更新日期：2007-05-22 00:00:00

  • Structure and catalytic mechanism of N(5),N(10)-methenyl-tetrahydromethanopterin cyclohydrolase.

    abstract：:Methenyltetrahydromethanopterin (methenyl-H(4)MPT(+)) cyclohydrolase (Mch) catalyzes the interconversion of methenyl-H(4)MPT(+) and formyl-H(4)MPT in the one-carbon energy metabolism of methanogenic, methanotrophic, and sulfate-reducing archaea and of methylotrophic bacteria. To understand the catalytic mechanism of t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300777k

    authors： Upadhyay V,Demmer U,Warkentin E,Moll J,Shima S,Ermler U

    更新日期：2012-10-23 00:00:00