Modified nucleosides in the first positions of the anticodons of tRNA(Leu)4 and tRNA(Leu)5 from Escherichia coli.

abstract：:In Bacillus subtilis, P protein is the noncatalytic component of ribonuclease P (RNase P) that is critical for achieving maximal nuclease activity under physiological conditions. P protein is predominantly unfolded (D) at neutral pH and low ionic strength; however, it folds upon the addition of sulfate anions (ligands...

journal_title：Biochemistry

authors： Henkels CH,Oas TG

更新日期：2005-10-04 00:00:00

abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

journal_title：Biochemistry

authors： Santoro MM,Bolen DW

更新日期：1992-05-26 00:00:00

abstract：:To describe the time course of cellular systems, we integrate ideas from thermodynamics and information theory to discuss the work needed to change the state of the cell. The biological example analyzed is experimental microarray transcription level oscillations of yeast in the different phases as characterized by oxy...

journal_title：Biochemistry

authors： Gross A,Li CM,Remacle F,Levine RD

更新日期：2013-03-05 00:00:00

abstract：:One- and two-dimensional NMR experiments were carried out on a decamer, d-(CGCTTTTCGC).d(GCGAAAAGCG), and on the same sequence with the addition of an unpaired thymidine, d(CGCTTTTCGC).d(GCGAATAAGCG), which will be referred to as the T-bulge decamer. Evidence from one-dimensional NOE experiments on the exchangeable pr...

journal_title：Biochemistry

authors： Morden KM,Gunn BM,Maskos K

更新日期：1990-09-18 00:00:00

abstract：:The transfer of cytochrome P-450 from bovine adrenocortical submitochondrial particles (smp) to unilamellar liposome membranes was investigated using a table top ultracentrifuge. Submitochondrial particles were incubated with liposome membranes at 25 degrees C and precipitated by ultracentrifugation at 200000g for a f...

journal_title：Biochemistry

authors： Kominami S,Onizuka M,Tasaka-Marumoto C,Takemori S

更新日期：1995-04-11 00:00:00

abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...

journal_title：Biochemistry

authors： Querfurth HW,Selkoe DJ

更新日期：1994-04-19 00:00:00

abstract：:Glycopeptidolipids (GPLs) are specific constituents of mycobacteria known as opportunistic pathogens. The influence of the carbohydrate moiety on GPL-induced membrane alterations was examined with GPLs bearing 1-5 sugar residues (GPL-1 to GPL-5) and a sulfated GPL (S-GPL-2). GPLs decreased the ADP/O ratio and increase...

journal_title：Biochemistry

authors： Lopez-Marin LM,Quesada D,Lakhdar-Ghazal F,Tocanne JF,Lanéelle G

更新日期：1994-06-14 00:00:00

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

journal_title：Biochemistry

authors： Yano Y,Matsuzaki K

更新日期：2002-10-15 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:The growth and shortening of microtubules in guanosine triphosphate-(GTP-) mediated dynamic instability has previously been observed to occur at rates which are remarkably variable (Gildersleeve et al., 1992, Chrétien et al., 1995). Neighboring microtubules observed simultaneously can grow or shorten at different rate...

journal_title：Biochemistry

authors： Dye RB,Williams RC Jr

更新日期：1996-11-12 00:00:00

abstract：:Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhe...

journal_title：Biochemistry

authors： Lawless MK,Barney S,Guthrie KI,Bucy TB,Petteway SR Jr,Merutka G

更新日期：1996-10-22 00:00:00

abstract：:BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...

journal_title：Biochemistry

authors： Doerner PA,Sousa MC

更新日期：2017-06-20 00:00:00

abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...

journal_title：Biochemistry

authors： Lefferts JA,Wang C,Sridharan D,Baralt M,Lambert MW

更新日期：2009-01-20 00:00:00

abstract：:Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...

journal_title：Biochemistry

authors： Corbin JA,Dirkx RA,Falke JJ

更新日期：2004-12-28 00:00:00

abstract：:A steady-state kinetic analysis was conducted of the overall aminoacylation reaction catalyzed by isoleucyl-tRNA synthetase. The patterns of Lineweaver-Burk plots obtained indicated that tRNA adds to the enzyme only after isoleucyl adenylate formation and pyrophosphate release. These kinetic patterns were consistent w...

journal_title：Biochemistry

authors： Moe JG,Piszkiewicz D

更新日期：1979-06-26 00:00:00

abstract：:Comparative electrophoretic, thermal denaturation, and spectroscopic studies with dodecamers of the form d(ATTA-XGCX-TAAT) and their self-complementary counterparts suggest that actinomycin D (ACTD) binds strongly to a 5'GC3' site with flanking T/T mismatches and moderately to that with C/C mismatches but weakly to th...

journal_title：Biochemistry

authors： Liu C,Chen FM

更新日期：1996-12-17 00:00:00

abstract：:Limited cleavages of human C1r by extrinsic proteases of various specificity (plasmin, elastase, chymotrypsin, thermolysin) yield dimeric associations of two globular domains, each comprised of the intact B chain disulfide linked to gamma, the C-terminal fragment of the A chain. These (gamma-B)2 domains, which are hom...

journal_title：Biochemistry

authors： Arlaud GJ,Gagnon J,Villiers CL,Colomb MG

更新日期：1986-09-09 00:00:00

abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....

journal_title：Biochemistry

authors： Kim S,Merrill BM,Rajpurohit R,Kumar A,Stone KL,Papov VV,Schneiders JM,Szer W,Wilson SH,Paik WK,Williams KR

更新日期：1997-04-29 00:00:00

abstract：:Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...

journal_title：Biochemistry

authors： Bazzi MD,Nelsestuen GL

更新日期：1988-10-04 00:00:00

abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

journal_title：Biochemistry

authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

更新日期：2011-10-11 00:00:00

abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...

journal_title：Biochemistry

authors： Horng JC,Moroz V,Rigotti DJ,Fairman R,Raleigh DP

更新日期：2002-11-12 00:00:00

abstract：:To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...

journal_title：Biochemistry

authors： Himes RD,Smolin N,Kukol A,Bossuyt J,Bers DM,Robia SL

更新日期：2016-11-08 00:00:00

abstract：:Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...

journal_title：Biochemistry

authors： Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

更新日期：1997-08-05 00:00:00

abstract：:The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

journal_title：Biochemistry

authors： Babé LM,Pichuantes S,Craik CS

更新日期：1991-01-08 00:00:00

abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Gomes CM,Teixeira M,Brzezinski P

更新日期：1999-08-03 00:00:00

abstract：:DNA gyrase is the target of a number of antibacterial agents, including the coumarins and the cyclothialidines. To extend our understanding of the mechanism of action of these compounds, we have examined the previously published crystal structures of the complexes between the 24 kDa fragment of GyrB and coumarin and c...

journal_title：Biochemistry

authors： Kampranis SC,Gormley NA,Tranter R,Orphanides G,Maxwell A

更新日期：1999-02-16 00:00:00

abstract：:Vanadate can often bind to phosphoryl transfer enzymes to form a trigonal-bipyramidal structure at the active site. The enzyme-vanadate dissociation constants in these enzymes are much lower than those for phosphate. Therefore, enzyme-bound vanadate moieties are often considered as transition state analogues. To test ...

journal_title：Biochemistry

authors： Deng H,Callender R,Huang Z,Zhang ZY

更新日期：2002-05-07 00:00:00

abstract：:We propose a simple method to find an amino acid sequence that is foldable into a globular protein with a desired structure based on a knowledge-based 3D-1D compatibility function. An asymmetric alpha-helical single-domain structure of sperm whale myoglobin consisting of 153 amino acid residues was chosen for the desi...

journal_title：Biochemistry

authors： Isogai Y,Ota M,Fujisawa T,Izuno H,Mukai M,Nakamura H,Iizuka T,Nishikawa K

更新日期：1999-06-08 00:00:00