Interactions of mycobacterial glycopeptidolipids with membranes: influence of carbohydrate on induced alterations.


:Glycopeptidolipids (GPLs) are specific constituents of mycobacteria known as opportunistic pathogens. The influence of the carbohydrate moiety on GPL-induced membrane alterations was examined with GPLs bearing 1-5 sugar residues (GPL-1 to GPL-5) and a sulfated GPL (S-GPL-2). GPLs decreased the ADP/O ratio and increased controlled respiration of isolated mitochondria. The more polar GPLs were the less active, with the following order of efficiency: GPL-1 > GPL-2 > S-GPL-2 = GPL-3 = GPL-5. GPL-1 and GPL-2 increased passive permeability of liposomes to carboxyfluorescein (GPL-1 > GPL-2), while GPL-3 and GPL-5 were inactive. GPL-2 and GPL-3 decreased the transmembrane electrical potential (delta psi) in isolated mitochondria (GPL-2 > GPL-3). These results suggest that GPLs uncouple oxidative phosphorylation by increasing the passive permeability of the mitochondrial membrane to protons. Compression isotherms of GPL-2 monolayers showed that, at low surface pressure, the area per GPL-2 molecule was about 5 times that of an acyl chain: it is likely that the peptide moiety was at the air/water interface. With an increase in the surface pressure, its area decreased, down to that of a tightly packed acyl chain. It is postulated that the glycopeptidic moiety can be either at in the interface or dipping into the water.(ABSTRACT TRUNCATED AT 250 WORDS)






Lopez-Marin LM,Quesada D,Lakhdar-Ghazal F,Tocanne JF,Lanéelle G




Has Abstract


1994-06-14 00:00:00












  • Interaction of profilin with G-actin and poly(L-proline).

    abstract::The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...


    pub_type: 杂志文章


    authors: Perelroizen I,Marchand JB,Blanchoin L,Didry D,Carlier MF

    更新日期:1994-07-19 00:00:00

  • Contribution of light chain residues to high affinity binding in an HIV-1 antibody explored by combinatorial scanning mutagenesis.

    abstract::Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...


    pub_type: 杂志文章


    authors: Da Silva GF,Harrison JS,Lai JR

    更新日期:2010-07-06 00:00:00

  • Sterol orientations in phosphatidylcholine liposomes as determined by deuterium NMR.

    abstract::Deuterium magnetic resonance spectra (55.26 MHz) of cholesterol-3 alpha-d1 and epicholesterol-3 beta-d1 in dipalmitoylglycerophosphocholine (DPPC) liposomes were measured as a function of sterol-to-phospholipid ratio below (24 degrees C) and above (60 degrees C) the phase transition temperature of DPPC. From the quadr...


    pub_type: 杂志文章


    authors: Murari R,Murari MP,Baumann WJ

    更新日期:1986-03-11 00:00:00

  • Single-molecule visualization of environment-sensitive fluorophores inserted into cell membranes by staphylococcal gamma-hemolysin.

    abstract::Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...


    pub_type: 杂志文章


    authors: Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

    更新日期:2006-02-28 00:00:00

  • Role of the covalent flavin linkage in monomeric sarcosine oxidase.

    abstract::Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...


    pub_type: 杂志文章


    authors: Hassan-Abdallah A,Zhao G,Jorns MS

    更新日期:2006-08-08 00:00:00

  • A single-molecule view of the assembly pathway, subunit stoichiometry, and unwinding activity of the bacteriophage T4 primosome (helicase-primase) complex.

    abstract::Single-molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks "internally" labeled in the duplex r...


    pub_type: 杂志文章


    authors: Lee W,Jose D,Phelps C,Marcus AH,von Hippel PH

    更新日期:2013-05-07 00:00:00

  • Effects of the A.T/T.A degeneracy of pyrrole--imidazole polyamide recognition in the minor groove of DNA.

    abstract::Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G.C from C.G and both of these from A.T/T.A base pairs. A Py/Py pair appears not to distinguish A.T from T.A base pairs. To test t...


    pub_type: 杂志文章


    authors: White S,Baird EE,Dervan PB

    更新日期:1996-09-24 00:00:00

  • Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phospho

    abstract::An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...


    pub_type: 杂志文章


    authors: Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

    更新日期:2001-11-13 00:00:00

  • Oxidative chemistry in the GFP active site leads to covalent cross-linking of a modified leucine side chain with a histidine imidazole: implications for the mechanism of chromophore formation.

    abstract::The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...


    pub_type: 杂志文章


    authors: Rosenow MA,Patel HN,Wachter RM

    更新日期:2005-06-14 00:00:00

  • Protoheme-carbon monoxide geminate kinetics.

    abstract::Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...


    pub_type: 杂志文章


    authors: Marden MC,Hazard ES 3rd,Gibson QH

    更新日期:1986-05-20 00:00:00

  • Influence of chemistry in immobilization of cobra venom phospholipase A2: implications as to mechanism.

    abstract::Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, h...


    pub_type: 杂志文章


    authors: Ferreira JP,Sasisekharan R,Louie O,Langer R

    更新日期:1993-08-17 00:00:00

  • Purification and properties of nuclease SP.

    abstract::Single-strand-specific nucleases are a diverse and important group of enzymes that are able to cleave a variety of DNA structures present in duplex molecules. Nuclease SP, an enzyme from spinach, has been purified to apparent homogeneity, allowing for the unambiguous characterization of a number of its physical proper...


    pub_type: 杂志文章


    authors: Strickland JA,Marzilli LG,Puckett JM Jr,Doetsch PW

    更新日期:1991-10-08 00:00:00

  • Analysis of cooperativity observed in pH titrations of proton nuclear magnetic resonances of histidine residues of rabbit cardiac tropomyosin.

    abstract::We have investigated in detail the cooperativity which we had previously observed in the pH titration profiles of the histidine residues of rabbit tropomyosin [Edwards, B. F. P., & Sykes, B. D. (1978) Biochemistry 17, 684]. Nonpolymerizing tropomyosin was prepared by carboxypeptidase digestion, and the titration profi...


    pub_type: 杂志文章


    authors: Edwards BF,Sykes BD

    更新日期:1981-07-07 00:00:00

  • Mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase.

    abstract::Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...


    pub_type: 杂志文章


    authors: Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

    更新日期:2013-08-13 00:00:00

  • Structural Basis for Excision of 5-Formylcytosine by Thymine DNA Glycosylase.

    abstract::Thymine DNA glycosylase (TDG) is a base excision repair enzyme with key functions in epigenetic regulation. Performing a critical step in a pathway for active DNA demethylation, TDG removes 5-formylcytosine and 5-carboxylcytosine, oxidized derivatives of 5-methylcytosine that are generated by TET (ten-eleven transloca...


    pub_type: 杂志文章


    authors: Pidugu LS,Flowers JW,Coey CT,Pozharski E,Greenberg MM,Drohat AC

    更新日期:2016-11-15 00:00:00

  • Inhibition of islet amyloid polypeptide fibril formation: a potential role for heteroaromatic interactions.

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    pub_type: 杂志文章


    authors: Porat Y,Mazor Y,Efrat S,Gazit E

    更新日期:2004-11-16 00:00:00

  • Stacking of bacteriochlorophyll c macrocycles in chlorosome from Chlorobium limicola as revealed by intermolecular 13C magnetic-dipole correlation, X-ray diffraction, and quadrupole coupling in 25Mg NMR.

    abstract::The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...


    pub_type: 杂志文章


    authors: Kakitani Y,Koyama Y,Shimoikeda Y,Nakai T,Utsumi H,Shimizu T,Nagae H

    更新日期:2009-01-13 00:00:00

  • A homozygous human cell line contains three subsets of HLA-DR-like antigens distinguishable by amino acid sequencing.

    abstract::The major histocompatibility complex (HLA in humans) contains a relatively large set of gene loci in the HLA-D/DR region responsible for regulation of the immune response. The structural dissection of the protein products of these loci is a necessary accompaniment to understanding of this response. In this study, two ...


    pub_type: 杂志文章


    authors: Andrews DW,Bono MR,Strominger JL

    更新日期:1982-12-21 00:00:00

  • Oxygen gradients in mitochondria examined with delayed luminescence from excited-state triplet probes.

    abstract::Phosphorescent probes are described that are quenchable by dioxygen and that partition into membranes. These probes are derivatives of porphyrin, in which the central metal, either zinc or palladium, induces intersystem crossing to enhance the triplet yield. The location of the probe in a suspension of membranes depen...


    pub_type: 杂志文章


    authors: Vanderkooi JM,Wright WW,Erecinska M

    更新日期:1990-06-05 00:00:00

  • Engineering of the nonspecific phospholipase C from Bacillus cereus: replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity.

    abstract::The nonspecific phospholipase C from Bacillus cereus is a zinc metalloenzyme that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. Glu-4 has been proposed as a potential candidate for the general base in the hydrolysis reaction and was shown to interact with the substrate he...


    pub_type: 杂志文章


    authors: Tan CA,Roberts MF

    更新日期:1998-03-24 00:00:00

  • Multivector fluorescence analysis of the xpt guanine riboswitch aptamer domain and the conformational role of guanine.

    abstract::Purine riboswitches are RNA regulatory elements that control purine metabolism in response to intracellular concentrations of the purine ligands. Conformational changes of the guanine riboswitch aptamer domain induced by guanine binding lead to transcriptional regulation of genes involved in guanine biosynthesis. The ...


    pub_type: 杂志文章


    authors: Brenner MD,Scanlan MS,Nahas MK,Ha T,Silverman SK

    更新日期:2010-03-02 00:00:00

  • Binding of proteins to specific target sites in membranes measured by total internal reflection fluorescence microscopy.

    abstract::A new quantitative technique for measuring the binding of proteins to membranes is described. The method is based on a combination of total internal reflection fluorescence microscopy and the preparation of supported planar bilayers. Specific and reversible binding of a fluorescence-labeled monoclonal antibody to lipi...


    pub_type: 杂志文章


    authors: Kalb E,Engel J,Tamm LK

    更新日期:1990-02-13 00:00:00

  • Effects of biological oxidants on the catalytic activity and structure of group VIA phospholipase A2.

    abstract::Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...


    pub_type: 杂志文章


    authors: Song H,Bao S,Ramanadham S,Turk J

    更新日期:2006-05-23 00:00:00

  • Nucleotide Excision Repair and Impact of Site-Specific 5',8-Cyclopurine and Bulky DNA Lesions on the Physical Properties of Nucleosomes.

    abstract::The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...


    pub_type: 杂志文章


    authors: Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

    更新日期:2019-02-12 00:00:00

  • FTIR spectroscopic characterization of the cytochrome aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton translocation.

    abstract::The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...


    pub_type: 杂志文章


    authors: Hellwig P,Gomes CM,Teixeira M

    更新日期:2003-05-27 00:00:00

  • Structural studies of the contractile tail sheath protein of bacteriophage T4. 2. Structural analyses of the tail sheath protein, Gp18, by limited proteolysis, immunoblotting, and immunoelectron microscopy.

    abstract::The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...


    pub_type: 杂志文章


    authors: Arisaka F,Takeda S,Funane K,Nishijima N,Ishii S

    更新日期:1990-05-29 00:00:00

  • Molecular interactions between human lactotransferrin and the phytohemagglutinin-activated human lymphocyte lactotransferrin receptor lie in two loop-containing regions of the N-terminal domain I of human lactotransferrin.

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    pub_type: 杂志文章


    authors: Legrand D,Mazurier J,Elass A,Rochard E,Vergoten G,Maes P,Montreuil J,Spik G

    更新日期:1992-09-29 00:00:00

  • Bleomycin-DNA interactions: fluorescence and proton magnetic resonance studies.

    abstract::The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...


    pub_type: 杂志文章


    authors: Chien M,Grollman AP,Horwitz SB

    更新日期:1977-08-09 00:00:00

  • Vibrationally enhanced hydrogen tunneling in the Escherichia coli thymidylate synthase catalyzed reaction.

    abstract::The enzyme thymidylate synthase (TS) catalyzes a complex reaction that involves forming and breaking at least six covalent bonds. The physical nature of the hydride transfer step in this complex reaction cascade has been studied by means of isotope effects and their temperature dependence. Competitive kinetic isotope ...


    pub_type: 杂志文章


    authors: Agrawal N,Hong B,Mihai C,Kohen A

    更新日期:2004-02-24 00:00:00

  • Extreme Dynamics in the BamA β-Barrel Seam.

    abstract::BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...


    pub_type: 杂志文章


    authors: Doerner PA,Sousa MC

    更新日期:2017-06-20 00:00:00