Abstract:
:Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhelical, and that the inhibitory action of these peptides occurs after the interaction between the viral gp120 protein and the cellular CD4 receptor [Wild, C.T., Shugars, D. C., Greenwell, T. K., McDanal, C. B., Matthews, T. J. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 9770 and references therein]. The current study uses sedimentation equilibrium (SE), circular dichroism (CD), and viral-fusion assays to quantitatively investigate peptide structure and peptide-peptide interactions. SE analyses of T21 (1-100 microM) indicate that the peptide self associates via a monomer/dimer/tetramer equilibrium; in addition, T20 is monomeric in the range of 1-10 microM and exhibits a complicated monomer/tetramer equilibrium between 20 and 100 microM. Singular value decomposition analyses of the CD spectra of T21 and T20 indicate that the helical content of these peptides in PBS solution is 90% and 20%, respectively. A structural interaction between the two peptides is detected by CD at several concentration ratios of T20:T21. These experiments emphasize that T20 interacts specifically with the tetrameric form of T21. Truncated forms of T20 also exhibit structural interactions with T21 at varying concentration ratios. The ability of T20 and the truncated peptides to interact structurally with tetrameric T21 correlates with antiviral activity. Implications of these findings are discussed in terms of proposed mechanisms of membrane fusion inhibition and the structural changes which occur in gp41 during membrane fusion.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lawless MK,Barney S,Guthrie KI,Bucy TB,Petteway SR Jr,Merutka Gdoi
10.1021/bi9606962subject
Has Abstractpub_date
1996-10-22 00:00:00pages
13697-708issue
42eissn
0006-2960issn
1520-4995pii
bi9606962journal_volume
35pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The S-adenosylmethionine (SAM) riboswitch is one of the most recurrent riboswitches found in bacteria and has three known different natural aptamers. The Bacillus subtilis yitJ SAM riboswitch aptamer is organized around a four-way junction which is characterized by the presence of a pseudoknot and a K-turn motif. By r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701164y
更新日期:2008-02-12 00:00:00
abstract::A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700596s
更新日期:2007-07-17 00:00:00
abstract::The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a058
更新日期:1985-07-16 00:00:00
abstract::The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051767c
更新日期:2006-01-10 00:00:00
abstract::The Ca2+-dependent, reversible, interaction of cyclic adenosine 3',5'-monophosphate (cAMP) phosphodiesterase with its activator has been used to purify the enzyme by affinity chromatography. Activator-dependent cAMP phosphodiesterase is only a minor component of the proteins specifically adsorbed in the presence of Ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00594a017
更新日期:1978-01-10 00:00:00
abstract::The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00550a021
更新日期:1980-04-29 00:00:00
abstract::The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00378a023
更新日期:1987-02-24 00:00:00
abstract::Electron transfer between the Rieske iron-sulfur protein (Fe(2)S(2)) and cytochrome c(1) was studied using the ruthenium dimer, Ru(2)D, to either photoreduce or photooxidize cytochrome c(1) within 1 micros. Ru(2)D has a charge of +4, which allows it to bind with high affinity to the cytochrome bc(1) complex. Flash pho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000003o
更新日期:2000-04-18 00:00:00
abstract::The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00344a013
更新日期:1985-11-05 00:00:00
abstract::Protein kinases are key regulators of diverse signaling networks critical for growth and development. Protein kinase A (PKA) is an important kinase prototype that phosphorylates protein targets at Ser and Thr residues by converting ATP to ADP. Mg(2+) ions play a crucial role in regulating phosphoryl transfer and can l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901475g
更新日期:2009-12-08 00:00:00
abstract::In eukaryotic cells, structures called heterochromatin play critical roles in nuclear processes ranging from gene repression to chromosome segregation. Biochemical and in vivo studies over the past several decades have implied that the diverse functions of heterochromatin rely on the ability of these structures to spr...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.8b00401
更新日期:2018-05-01 00:00:00
abstract::Ala-based peptides form marginally stable helices at low temperature and are conventionally considered as mixtures of alpha-helix and random coil. However, recent work with doubly spin-labeled peptides suggests that short 16-residue sequences contain a significant fraction of 3(10)-helix near the N-terminus (positions...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00096a003
更新日期:1993-11-16 00:00:00
abstract::Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00354a031
更新日期:1986-03-25 00:00:00
abstract::The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00500a008
更新日期:1990-12-04 00:00:00
abstract::The major histocompatibility complex (HLA in humans) contains a relatively large set of gene loci in the HLA-D/DR region responsible for regulation of the immune response. The structural dissection of the protein products of these loci is a necessary accompaniment to understanding of this response. In this study, two ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00269a002
更新日期:1982-12-21 00:00:00
abstract::By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a020
更新日期:1988-05-31 00:00:00
abstract::Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00057a003
更新日期:1993-02-16 00:00:00
abstract::Two tetrapeptide sequence homologies between mouse epidermal growth factor precursor (mEGFP) and human follitropin (FSH) were revealed by a computer program that identifies identical residues among polypeptide sequences. The two tetrapeptides, Lys-Thr-Cys-Thr (KTCT) and Thr-Arg-Asp-Leu (TRDL), are present in the hormo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00357a053
更新日期:1986-05-06 00:00:00
abstract::In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00184a028
更新日期:1994-05-10 00:00:00
abstract::The chemical shifts of all the aromatic proton and anomeric proton resonances of NADP+, NADPH, and several structural analogues have been determined in their complexes with Lactobacillus casei dihydrofolate reductase by double-resonance (saturation transfer) experiments. The binding of NADP+ to the enzyme leads to lar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00557a015
更新日期:1980-08-05 00:00:00
abstract::Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00848
更新日期:2017-12-19 00:00:00
abstract::The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010938k
更新日期:2001-08-21 00:00:00
abstract::The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200207w
更新日期:2011-05-17 00:00:00
abstract::Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9704212
更新日期:1997-07-22 00:00:00
abstract::The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00066a004
更新日期:1993-04-20 00:00:00
abstract::Signal transduction in B cells is mediated, in part, by the interaction of the cytoplasmic components of the antigen receptor complex and various members of the src family tyrosine kinases. Key to this process appears to be the interaction of the tyrosine kinase SH2 domains with the tyrosine-phosphorylated cytoplasmic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960157x
更新日期:1996-05-21 00:00:00
abstract::Effects of different end sequences on melting, circular dichroism spectra (CD), and enzyme binding properties were investigated for four 40 base pair, non-self-complementary duplex DNA oligomers. The center sequences of these oligoduplexes have either of two 22 base pair modules flanked on both sides by sequences diff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000326k
更新日期:2000-07-04 00:00:00
abstract::A type II topoisomerase is essential for decatenating DNA replication products, and it accomplishes this task by passing one DNA duplex through a transient break in a second duplex. The B' domain of topoisomerase II contains three highly conserved motifs, EGDSA, PL(R/K)GK(I/L/M)LNVR, and IMTD(Q/A)DXD. We have investig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991328b
更新日期:2000-02-15 00:00:00
abstract::The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0622165
更新日期:2007-03-06 00:00:00
abstract::Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300039f
更新日期:2012-04-10 00:00:00