DNA strand arrangement within the SfiI-DNA complex: atomic force microscopy analysis.

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Human granulocyte chemotactic protein 2 (GCP-2) has originally been isolated from cytokine-stimulated osteosarcoma cells as a chemokine coproduced in minute amounts together with interleukin 8. Human GCP-2 (75 residues) was synthesized on a 0.25-mmol scale using Fmoc chemistry. After disulfide bridge formation and pur...

journal_title：Biochemistry

authors： Wuyts A,Van Osselaer N,Haelens A,Samson I,Herdewijn P,Ben-Baruch A,Oppenheim JJ,Proost P,Van Damme J

更新日期：1997-03-04 00:00:00

abstract：:Pineapple stem bromelain was photooxidized in the presence of Methylene Blue used as a sensitizer. The essential sulfhydryl group of the enzyme protein rapidly became inaccessible to react with 5,5'-dithiobis(nitrobenzoic acid), but the reactivity was readily regained to the original level upon treatment with dithioth...

journal_title：Biochemistry

authors： Murachi T,Tsudzuki T,Okumura K

更新日期：1975-01-28 00:00:00

abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

journal_title：Biochemistry

authors： Kitajima Y,Noguchi T

更新日期：2006-02-14 00:00:00

abstract：:Electron transfer in reaction center core (RCC) complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum was studied by measuring flash-induced absorbance changes. The first preparation contained approximately three iron-sulfur centers, indicating that the three putative electron acce...

journal_title：Biochemistry

authors： Schmidt KA,Neerken S,Permentier HP,Hager-Braun C,Amesz J

更新日期：2000-06-20 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...

journal_title：Biochemistry

authors： Lin H,Lal R,Clegg DO

更新日期：2000-03-28 00:00:00

abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...

journal_title：Biochemistry

authors： Jiang W,Locke G,Harpel MR,Copeland RA,Marcinkeviciene J

更新日期：2000-07-11 00:00:00

abstract：:A gene encoding the high-potential iron-sulfur protein (HiPIP) was cloned from the purple photosynthetic bacterium Rubrivivax gelatinosus. An insertional disruption of this gene by a kanamycin resistance cartridge resulted in a significant decrease in the growth rate under photosynthetic growth conditions. Flash-induc...

journal_title：Biochemistry

authors： Nagashima KV,Matsuura K,Shimada K,Verméglio A

更新日期：2002-11-26 00:00:00

abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

journal_title：Biochemistry

authors： Brandes R,Vold RR,Vold RL,Kearns DR

更新日期：1986-11-18 00:00:00

abstract：:The conformational conversion of prion protein (PrP) from an alpha-helix-rich normal cellular isoform (PrPC) to a beta-sheet-rich pathogenic isoform (PrPSc) is a key event in the development of prion diseases, and it takes place in caveolae, cavelike invaginations of the plasma membrane. A peptide homologous to residu...

journal_title：Biochemistry

authors： Miura T,Yoda M,Takaku N,Hirose T,Takeuchi H

更新日期：2007-10-16 00:00:00

abstract：:To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...

journal_title：Biochemistry

authors： Lee BK,Lee YH,Hauser M,Son CD,Khare S,Naider F,Becker JM

更新日期：2002-11-19 00:00:00

abstract：:Photosynthetic water oxidation takes place at the Mn4CaO5 cluster in photosystem II. Around the Mn4CaO5 cluster, a hydrogen bond network is formed by several water molecules, including four water ligands. To clarify the role of this water network in the mechanism of water oxidation, we investigated the effects of the ...

journal_title：Biochemistry

authors： Nakamura S,Ota K,Shibuya Y,Noguchi T

更新日期：2016-01-26 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

journal_title：Biochemistry

authors： Glaser PE,Gross RW

更新日期：1995-09-26 00:00:00

abstract：:(E)-4-(4-Chlorophenyl)-2-oxo-3-butenoic acid (CPB) was found to irreversibly inactivate brewers' yeast pyruvate decarboxylase (PDC, EC 4.1.1.1) in a biphasic, sigmoidal manner, as is found for the kinetic behavior of substrate. An expression was derived for two-site irreversible inhibition of allosteric enzymes, and t...

journal_title：Biochemistry

authors： Kuo DJ,Jordan F

更新日期：1983-08-02 00:00:00

abstract：:Non-heme diiron clusters occur in a number of enzymes (e.g., ribonucleotide reductase, methane monooxygenase, and Delta9-stearoyl-ACP desaturase) that activate O2 for chemically difficult oxidation reactions. In each case, a kinetically labile peroxo intermediate is believed to form when O2 reacts with the diferrous e...

journal_title：Biochemistry

authors： Moënne-Loccoz P,Baldwin J,Ley BA,Loehr TM,Bollinger JM Jr

更新日期：1998-10-20 00:00:00

abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...

journal_title：Biochemistry

authors： Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

更新日期：2005-10-25 00:00:00

abstract：:Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...

journal_title：Biochemistry

authors： Schübeler D,Mielke C,Maass K,Bode J

更新日期：1996-08-27 00:00:00

abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...

journal_title：Biochemistry

authors： Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JL

更新日期：2000-08-08 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is a single-subunit enzyme which has a C-terminal amino acid sequence of Phe-Ala-Phe-Ala883 (FAFA883). Closely related hydrophobic sequences are present at the C termini of seven other single-subunit RNA polymerases, including the mitochondrial RNA polymerase. Mutations at any of the fo...

journal_title：Biochemistry

authors： Gardner LP,Mookhtiar KA,Coleman JE

更新日期：1997-03-11 00:00:00

abstract：:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...

journal_title：Biochemistry

authors： Zang LH,Ghosh S,Maki AH

更新日期：1989-03-07 00:00:00

abstract：:The direct transfer of NADH between A-B pairs of dehydrogenases and also the dissociation of NADH from individual E-NADH complexes have been investigated by transient stopped-flow kinetic techniques. Such A-B transfers of NADH occur without the intermediate dissociation of coenzyme into the aqueous solvent environment...

journal_title：Biochemistry

authors： Srivastava DK,Bernhard SA

更新日期：1987-03-10 00:00:00

abstract：:The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...

journal_title：Biochemistry

authors： Li XM,Momsen MM,Smaby JM,Brockman HL,Brown RE

更新日期：2001-05-22 00:00:00

abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

journal_title：Biochemistry

authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

更新日期：2011-10-11 00:00:00

abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...

journal_title：Biochemistry

authors： Martinez KL,Corringer PJ,Edelstein SJ,Changeux JP,Mérola F

更新日期：2000-06-13 00:00:00

abstract：:The kinetics of reduction of free flavin semiquinones of the individual components of 1:1 covalent and electrostatic complexes of yeast ferric and ferryl cytochrome c peroxidase and ferric horse cytochrome c have been studied. Covalent cross-linking between the peroxidase and cytochrome c at low ionic strength results...

journal_title：Biochemistry

authors： Hazzard JT,Moench SJ,Erman JE,Satterlee JD,Tollin G

更新日期：1988-03-22 00:00:00

abstract：:This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti-[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5-[MC]G6-C7-T8-A9-C10-C11).d(G12-G13-T14++ +-A15-G16-C17-G18- A19-T20-G21-G22) duplex [designated (-)-trans-anti-[MC]dG.dC 11-mer duplex]. This adduc...

journal_title：Biochemistry

authors： Cosman M,Xu R,Hingerty BE,Amin S,Harvey RG,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-05-09 00:00:00

abstract：:Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...

journal_title：Biochemistry

authors： Smerdon SJ,Dodson GG,Wilkinson AJ,Gibson QH,Blackmore RS

更新日期：1991-06-25 00:00:00

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00