A new subfamily of agmatinases present in methanogenic Archaea is Fe(II) dependent.

abstract：:Oxygenic photosynthesis in plants involves highly reactive intermediates and byproducts that can damage the photosynthetic apparatus and other chloroplast constituents. The potential for damage is exacerbated when the amount of absorbed light exceeds the capacity for light energy utilization in photosynthesis, a condi...

journal_title：Biochemistry

authors： Holt NE,Fleming GR,Niyogi KK

更新日期：2004-07-06 00:00:00

abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

journal_title：Biochemistry

authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

更新日期：1995-09-12 00:00:00

abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...

journal_title：Biochemistry

authors： Malikayil JA,Lerch K,Armitage IM

更新日期：1989-04-04 00:00:00

abstract：:The hepatitis delta virus (HDV) ribozyme and HDV-like ribozymes are self-cleaving RNAs found throughout all kingdoms of life. These RNAs fold into a double-nested pseudoknot structure and cleave RNA, yielding 2',3'-cyclic phosphate and 5'-hydroxyl termini. The active site nucleotide C75 has a pK(a) shifted >2 pH units...

journal_title：Biochemistry

authors： Chen JH,Yajima R,Chadalavada DM,Chase E,Bevilacqua PC,Golden BL

更新日期：2010-08-10 00:00:00

abstract：:An improved purification procedure for the alpha 2 beta 2 complex of tryptophan synthase from Escherichia coli has been developed. It consists of DEAE-Sephacel chromatography, followed by hydrophobic chromatography on Sepharose CL 4B, and leads to material with a higher specific activity than reported previously. Inhi...

journal_title：Biochemistry

authors： Tschopp J,Kirschner K

更新日期：1980-09-16 00:00:00

abstract：:The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted...

journal_title：Biochemistry

authors： Hjörleifsson JG,Ásgeirsson B

更新日期：2017-09-26 00:00:00

abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

journal_title：Biochemistry

authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

更新日期：2007-12-18 00:00:00

abstract：:The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...

journal_title：Biochemistry

authors： Halsey JF,Cebra JJ,Biltonen RL

更新日期：1975-11-18 00:00:00

abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...

journal_title：Biochemistry

authors： Querfurth HW,Selkoe DJ

更新日期：1994-04-19 00:00:00

abstract：:Recognition of and binding to cholesterol on the host cell membrane is an initial step in the mechanism of numerous pathogens, including viruses, bacteria, and bacterial toxins; however, a viable method of inhibiting this interaction has not yet been uncovered. Here, we describe the mechanism by which a cholesterol re...

journal_title：Biochemistry

authors： Koufos E,Chang EH,Rasti ES,Krueger E,Brown AC

更新日期：2016-08-30 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:Calmodulin, the Ca(2+)-dependent activator of many cellular processes, contains two well-defined structural domains, each of which binds two Ca(2+) ions. In its Ca(2+)-free (apo) form, it provides an attractive model for studying mechanisms of protein unfolding, exhibiting two separable, reversible processes, indicati...

journal_title：Biochemistry

authors： Biekofsky RR,Martin SR,McCormick JE,Masino L,Fefeu S,Bayley PM,Feeney J

更新日期：2002-05-28 00:00:00

abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...

journal_title：Biochemistry

authors： Serpell LC,Blake CC,Fraser PE

更新日期：2000-10-31 00:00:00

abstract：:The characterization of cod somatolactin (SL), a new pituitary protein belonging to the growth hormone/prolactin family, is described. Cod SL has a molecular weight of 26 kDa and consists of 209 amino acids, of which eight are Cys. The protein has three disulfide bonds between residues Cys5-Cys15, Cys65-Cys181, and Cy...

journal_title：Biochemistry

authors： Rand-Weaver M,Noso T,Muramoto K,Kawauchi H

更新日期：1991-02-12 00:00:00

abstract：:The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...

journal_title：Biochemistry

authors： Sackett DL,Lippoldt RE

更新日期：1991-04-09 00:00:00

abstract：:With excitation at 406.7 nm, we have observed the resonance Raman enhancement of the bound v(N-O) stretch at approximately 1623 cm-1 in nitrosylhemoglobin A and nitrosylmyoglobin, indicating the existence of a charge-transfer transition underlying the strong Soret band. The v(Fe-NO) stretch at 551 cm-1 has also been d...

journal_title：Biochemistry

authors： Tsubaki M,Yu NT

更新日期：1982-03-16 00:00:00

abstract：:The purified ATP-synthesizing complex from chloroplasts has been reconstituted into phospholipid vesicles with bacteriorhodopsin by use of octyl glucoside. Phosphorylation rates up to 90 mmol of ATP (mg of protein)-1 min-1 have been achieved. The dependence of the steady-state kinetic parameters on external and intern...

journal_title：Biochemistry

authors： Takabe T,Hammes GG

更新日期：1981-11-24 00:00:00

abstract：:We present a new design of mixed-backbone antisense oligonucleotides (ASOs) containing both DNA and peptide nucleic acid (PNA). Previous generations of PNA-DNA chimeras showed low binding affinity, reducing their potential as therapeutics. The addition of a 5'-wing of locked nucleic acid as well as the combination of ...

journal_title：Biochemistry

authors： Debacker AJ,Sharma VK,Meda Krishnamurthy P,O'Reilly D,Greenhill R,Watts JK

更新日期：2019-02-12 00:00:00

abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

journal_title：Biochemistry

authors： Degani H,Shaer A,Victor TA,Kaye AM

更新日期：1984-06-05 00:00:00

abstract：:DNA binding modes of distamycin (DST) were investigated via comparative binding studies with oligomeric duplexes of the form d(GCG-X-GCG).d(CGC-Y-CGC), where Y is complementary to X and X = 4- or 5-base binding site. It was found that 1:1 and 2:1 drug-duplex complexes exhibit distinctly different circular dichroic (CD...

journal_title：Biochemistry

authors： Chen FM,Sha F

更新日期：1998-08-11 00:00:00

abstract：:The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...

journal_title：Biochemistry

authors： Pfeiffer DR,Hutson SM,Kauffman RF,Lardy HA

更新日期：1976-06-15 00:00:00

abstract：:The active site of histidine decarboxylase (HDC) from Lactobacillus 30a contains a pyruvoyl cofactor sitting at the interface of two molecules in a trimer. Although exhibiting hyperbolic kinetics at pH 4.8, near its optimum, HDC is cooperative at pH 7.6, indicating that the units of the trimer communicate. A Hill plot...

journal_title：Biochemistry

authors： Pishko EJ,Robertus JD

更新日期：1993-05-11 00:00:00

abstract：:The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then catalyzes the successive cleavage of the phosphodiester bonds first on the 3'-side and then on the 5'-side of the abasic site to generate 5'- and 3'-phosphate ends, ...

journal_title：Biochemistry

authors： Bhagwat M,Gerlt JA

更新日期：1996-01-16 00:00:00

abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

journal_title：Biochemistry

authors： Isambert MF,Gasnier B,Botton D,Henry JP

更新日期：1992-02-25 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...

journal_title：Biochemistry

authors： Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

更新日期：1990-09-04 00:00:00

abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...

journal_title：Biochemistry

authors： Smirnova IN,Kasho VN,Kaback HR

更新日期：2006-12-26 00:00:00

abstract：:Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

journal_title：Biochemistry

authors： Lee HC,Peisach J,Tsuneshige A,Yonetani T

更新日期：1995-05-23 00:00:00

abstract：:The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...

journal_title：Biochemistry

authors： Clore GM,Gronenborn AM,Nilges M,Ryan CA

更新日期：1987-12-01 00:00:00