Abstract：
:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quenching by noncovalent interaction with DNA. Fluorescence lifetimes of the free and intercalated states of these compounds were determined to be 9-10 and 0.5-1 ns, respectively. The bisintercalative effect of the dimeric analogue of Glu-P-2, bis(Glu-P-2)spermine (2GP-SP), to DNA was also investigated. This 2GP-SP, which has two Glu-P-2 moieties at each end of spermine, indicates a strong intramolecular interaction exhibiting remarkable quenching of fluorescence spectrum and lifetime (tau = 3.5 ns) in the absence of DNA. In the presence of DNA, however, the 3.5-ns lifetime component of fluorescence disappeared, and a two-exponential decay of fluorescence (t = approximately 10 and 1.5 ns) was observed at a DNA concentration of more than approximately 0.001 mM P, while the solution containing a very dilute DNA concentration (less than or equal to 0.001 mM P) exhibits a three-component decay of fluorescence (1.5, 3.5, and approximately 10 ns). The potent bis intercalation of two moieties in 2GP-SP with an identical DNA molecule was suggested by the DNA-concentration dependence of these fluorescence lifetimes and their intensity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo Kdoi
10.1021/bi00344a013subject
Has Abstractpub_date
1985-11-05 00:00:00pages
6401-5issue
23eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00415a030
更新日期：1988-07-26 00:00:00
abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00408a019
更新日期：1988-04-19 00:00:00
abstract：:We have examined slow changes in the rate of ATP hydrolysis for purified dog kidney Na+ and K+ stimulated adenosine triphosphatase [(Na-K)ATPase] at various concentrations of free Mg2+, Mg-ATP, K+, and Na+. The effect of these ligands on the rate of ATP hydrolysis is explained by a rapid binding step determining the i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00669a013
更新日期：1976-11-30 00:00:00
abstract：:Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9903345
更新日期：1999-07-20 00:00:00
abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700247y
更新日期：2007-06-19 00:00:00
abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00463a032
更新日期：1990-03-20 00:00:00
abstract：:Glutamate racemase, MurI, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of D-Glu for use in peptidoglycan biosynthesis. The enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anion...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002703z
更新日期：2001-05-29 00:00:00
abstract：:DNA gyrase and topoisomerase IV (Topo IV) are cellular targets of quinolone antibacterial drugs. The Ser-80 and the Glu-84 of the ParC subunit have been identified as mutational hotspots for quinolone resistance. Mutant Topo IV proteins containing a quinolone resistance-conferring mutation have been constructed, and t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026352v
更新日期：2002-10-01 00:00:00
abstract：:Neutral oligosaccharides, which accounted for 74% of the total N-linked sugar chains released by hydrazinolysis of rat small intestinal aminopeptidase N, were investigated on a structural basis. They are mainly composed of complex-type sugar chains with tri- and tetraantennary structures, and small amounts of high man...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00101a028
更新日期：1991-09-17 00:00:00
abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00376a019
更新日期：1987-01-27 00:00:00
abstract：:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972707c
更新日期：1998-02-24 00:00:00
abstract：:DNA polymerase β (Pol β) is a key enzyme in mammalian base excision repair (BER), contributing stepwise 5'-deoxyribose phosphate (dRP) lyase and "gap-filling" DNA polymerase activities. The lyase reaction is believed to occur via a β-elimination reaction following the formation of a Schiff base between the dRP group a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00807
更新日期：2017-01-24 00:00:00
abstract：:Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and map...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701914t
更新日期：2008-02-19 00:00:00
abstract：:To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026100u
更新日期：2002-11-19 00:00:00
abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a016
更新日期：1993-08-10 00:00:00
abstract：:The structural and functional properties of G protein-coupled receptors (GPCRs) are often studied in a detergent micellar environment, but many GPCRs tend to denature or aggregate in short alkyl chain detergents. In our previous work [Lee, S., et al. (2016) J. Am. Chem. Soc.138, 15425-15433], we showed that GPCRs in a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00183
更新日期：2020-06-16 00:00:00
abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0479003
更新日期：2005-01-18 00:00:00
abstract：:The conformation and activity of three different lipases have been studied in reverse micelles formed by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. In the case of human pancreatic lipase, the conformation of the polypeptide chain--as judged from far-UV circular dichroism measurements--is only slightly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00066a025
更新日期：1993-04-20 00:00:00
abstract：:The inhibitory effect of phosphorothioate residues, located within one strand of double-stranded DNA, on the hydrolytic activity of the restriction endonuclease EcoRV was investigated. Specific incorporation of a phosphorothioate group at the site of cleavage yielded the sequence 5'-GATsATC-3'. This modified sequence ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00493a008
更新日期：1990-10-16 00:00:00
abstract：:By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein bio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020352d
更新日期：2002-09-24 00:00:00
abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00201a018
更新日期：1994-09-06 00:00:00
abstract：:The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00514a035
更新日期：1981-05-26 00:00:00
abstract：:The lipid peroxidation product 4-hydroxynonenal (4-HNE) is a signaling mediator with wide-ranging biological effects. In this paper, we report that disruption of mGsta4, a gene encoding the 4-HNE-conjugating enzyme mGSTA4-4, causes increased 4-HNE tissue levels and is accompanied by age-dependent development of obesit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702124u
更新日期：2008-03-25 00:00:00
abstract：:The single reactive sulfhydryl group, located in the active site of each subunit of dimeric creatine kinase from rabbit muscle (isozyme MM), was selectively labeled with 3-(4-maleimidylphenyl)-7-(diethylamino)-4-methylcoumarin (CPM). Isozyme BB, purified to homogeneity from rabbit brain, was conjugated with the sulfhy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00292a018
更新日期：1983-11-08 00:00:00
abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528006
更新日期：1996-06-11 00:00:00
abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991497w
更新日期：1999-11-23 00:00:00
abstract：:Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050085m
更新日期：2005-08-09 00:00:00
abstract：:The photolyase/cryptochrome family is a large family of flavoproteins that encompasses DNA repair proteins, photolyases, and cryptochromes that regulate blue-light-dependent growth and development in plants, and light-dependent and light-independent circadian clock setting in animals. Phylogenetic analysis has reveale...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801085a
更新日期：2008-09-30 00:00:00
abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00337a032
更新日期：1985-07-30 00:00:00
abstract：:Calmodulin, the Ca(2+)-dependent activator of many cellular processes, contains two well-defined structural domains, each of which binds two Ca(2+) ions. In its Ca(2+)-free (apo) form, it provides an attractive model for studying mechanisms of protein unfolding, exhibiting two separable, reversible processes, indicati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012187s
更新日期：2002-05-28 00:00:00
