A slow interconversion between active and inactive states of the (Na-K)ATPase.

abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...

journal_title：Biochemistry

authors： Pina JM,Reynaga JM,Truong AAM,Keppetipola NM

更新日期：2018-07-03 00:00:00

abstract：:Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...

journal_title：Biochemistry

authors： Suh D,Povirk LF

更新日期：1997-04-08 00:00:00

abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

journal_title：Biochemistry

authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

更新日期：2008-08-05 00:00:00

abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...

journal_title：Biochemistry

authors： Zhang X,Adda CG,Low A,Zhang J,Zhang W,Sun H,Tu X,Anders RF,Norton RS

更新日期：2012-02-21 00:00:00

abstract：:We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...

journal_title：Biochemistry

authors： Rebecchi M,Boguslavsky V,Boguslavsky L,McLaughlin S

更新日期：1992-12-29 00:00:00

abstract：:The ovalbumin (Ov) gene contains a number of regulatory elements that control its transcriptional activity and restrict expression to avian oviduct. One major regulatory region, the steroid-dependent regulatory element (SDRE), is required for induction by estrogen and corticosterone. Another region, the negative regul...

journal_title：Biochemistry

authors： Park HM,Haecker SE,Hagen SG,Sanders MM

更新日期：2000-07-25 00:00:00

abstract：:Low pH-induced membrane insertion by diphtheria toxin T domain is crucial for A chain translocation into the cytoplasm. To define the membrane topography of the T domain, the exposure of biotinylated Cys residues to the cis and trans bilayer surfaces was examined using model membrane vesicles containing a deeply inser...

journal_title：Biochemistry

authors： Rosconi MP,Zhao G,London E

更新日期：2004-07-20 00:00:00

abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...

journal_title：Biochemistry

authors： Bhatnagar RS,Schall OF,Jackson-Machelski E,Sikorski JA,Devadas B,Gokel GW,Gordon JI

更新日期：1997-06-03 00:00:00

abstract：:ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...

journal_title：Biochemistry

authors： Roberts CS,Muralidharan S,Ni F,Mitra B

更新日期：2020-12-01 00:00:00

abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

journal_title：Biochemistry

authors： Bermejo GA,Strub MP,Ho C,Tjandra N

更新日期：2010-03-09 00:00:00

abstract：:Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), resp...

journal_title：Biochemistry

authors： Ofosu FA,Dewar L,Song Y,Cedrone AC,Hortelano G,Craven SJ

更新日期：2009-02-24 00:00:00

abstract：:A model cosolvent, ethanol, has profound and diversified effects on the amyloidogenic self-assembly of insulin, yielding spectroscopically and morphologically distinguishable forms of beta-aggregates. The alcohol reduces hydrodynamic radii of insulin molecules, decreases enthalpic costs associated with aggregation-pro...

journal_title：Biochemistry

authors： Dzwolak W,Grudzielanek S,Smirnovas V,Ravindra R,Nicolini C,Jansen R,Loksztejn A,Porowski S,Winter R

更新日期：2005-06-28 00:00:00

abstract：:Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...

journal_title：Biochemistry

authors： Jordan SP,Jorns MS

更新日期：1988-12-13 00:00:00

abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...

journal_title：Biochemistry

authors： Wales TE,Hochrein JM,Morgan CR,Emert-Sedlak LA,Smithgall TE,Engen JR

更新日期：2015-10-20 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl...

journal_title：Biochemistry

authors： Mancarella DA,Lenard J

更新日期：1981-11-24 00:00:00

abstract：:Room temperature phosphorescence techniques were used to study the structural and dynamic features of the tryptophan residues in bovine alpha-crystallin. Upon excitation at 290 nm, the characteristic signature of tryptophan phosphorescence was observed with an emission maximum at 442 +/- 2 nm. The phosphorescence inte...

journal_title：Biochemistry

authors： Berger JW,Vanderkooi JM

更新日期：1989-06-27 00:00:00

abstract：:The photochemical intermediate metarhodopsin II (meta II; lambda max = 380 nm) is generally identified with rho*, the conformation of photolyzed rhodopsin which binds and activates the visual G-protein, Gt [Emeis, D., & Hoffman, K.P. (1981) FEBS Lett. 136, 201-207]. Purified bovine rhodopsin was incorporated into vesi...

journal_title：Biochemistry

authors： Mitchell DC,Kibelbek J,Litman BJ

更新日期：1991-01-08 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...

journal_title：Biochemistry

authors： Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

更新日期：2015-09-29 00:00:00

abstract：:S-Adenosylmethionine (AdoMet) synthetase catalyzes the only known route of biosynthesis of the primary in vivo alkylating agent. Inhibitors of this enzyme could provide useful modifiers of biological methylation and polyamine biosynthetic processes. The AdoMet synthetase catalyzed reaction converts ATP and L-methionin...

journal_title：Biochemistry

authors： Reczkowski RS,Markham GD

更新日期：1999-07-13 00:00:00

abstract：:Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...

journal_title：Biochemistry

authors： Rajapaksha M,Kaur J,Bose M,Whittal RM,Bose HS

更新日期：2013-10-15 00:00:00

abstract：:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...

journal_title：Biochemistry

authors： Lijk LJ,Kalk KH,Brandenburg NP,Hol WG

更新日期：1983-06-07 00:00:00

abstract：:Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...

journal_title：Biochemistry

authors： Shaw JM,Thompson TE

更新日期：1982-03-02 00:00:00

abstract：:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...

journal_title：Biochemistry

authors： Zang LH,Ghosh S,Maki AH

更新日期：1989-03-07 00:00:00

abstract：:We describe a series of indolequinones as efficient mechanism-based inhibitors of NRH:quinone oxidoreductase 2 (NQO2) for use either in cellular or cell-free systems. Compounds were designed to be reduced in the active site of the enzyme leading to loss of a substituted phenol leaving group and generation of a reactiv...

journal_title：Biochemistry

authors： Yan C,Dufour M,Siegel D,Reigan P,Gomez J,Shieh B,Moody CJ,Ross D

更新日期：2011-08-09 00:00:00

abstract：:Apolipoprotein B (apoB) is the essential nonexchangeable protein in chylomicrons and very low-density lipoprotein-derived lipoprotein particles, including low-density lipoprotein (LDL). ApoB has been a key target for cardiovascular research because of its essential role in the assembly, secretion, delivery, and recept...

journal_title：Biochemistry

authors： Jiang ZG,Carraway M,McKnight CJ

更新日期：2005-02-01 00:00:00

abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

journal_title：Biochemistry

authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

更新日期：2013-02-26 00:00:00

abstract：:The mammalian HIRA/UBN1/CABIN1/ASF1a (HUCA) histone chaperone complex deposits the histone H3 variant H3.3 into chromatin and is linked to gene activation, repression, and chromatin assembly in diverse cell contexts. We recently reported that a short N-terminal fragment of UBN1 containing amino acids 1-175 is necessar...

journal_title：Biochemistry

authors： Tang Y,Puri A,Ricketts MD,Rai TS,Hoffmann J,Hoi E,Adams PD,Schultz DC,Marmorstein R

更新日期：2012-03-27 00:00:00

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

journal_title：Biochemistry

authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

更新日期：1998-11-10 00:00:00