Abstract：
:Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and mapped H-NS binding sites. Cnu consists of three alpha helices that are comparable with those of Hha, but it has significant flexibility in the C-terminal region and lacks a short alpha helix present in Hha. Upon increasing ionic strength, the helical structure of Cnu is destabilized, especially at the ends of the helices. The dominant H-NS binding sites, located at helix 3 as in Hha, reveal a common structural platform for H-NS binding. Our results may provide structural and dynamic bases for the similarity and dissimilarity between Cnu and Hha functions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bae SH,Liu D,Lim HM,Lee Y,Choi BSdoi
10.1021/bi701914tsubject
Has Abstractpub_date
2008-02-19 00:00:00pages
1993-2001issue
7eissn
0006-2960issn
1520-4995journal_volume
47pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a061
更新日期：1987-06-16 00:00:00
abstract：:Previously, we have described significant effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae under conditions of growth limitation. These regulations apparently rely on a transmembrane receptor capable of binding human insulin and responding by tyrosine/serine phosphorylation of a spe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9920432
更新日期：2000-02-15 00:00:00
abstract：:Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5'-diphosphates (NDPs) to deoxynucleotides (dNDPs). The active site for NDP reduction resides in α2, and the essential diferric-tyrosyl radical (Y(122)(•)) cofactor that initiates transfer of the radical to the act...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101319v
更新日期：2011-03-01 00:00:00
abstract：:To identify regions of the Torpedo Na,K-ATPase alpha-subunit that interact with membrane lipid and to characterize conformationally dependent structural changes in the transmembrane domain, we have proteolytically mapped the sites of photoincorporation of the hydrophobic compounds 3-(trifluoromethyl)-3-(m-[(125)I]iodo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0015461
更新日期：2000-11-07 00:00:00
abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a048
更新日期：1989-01-10 00:00:00
abstract：:HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960547j
更新日期：1996-10-01 00:00:00
abstract：:The hydrogen-bonded ring NH nuclear magnetic resonance (NMR) spectra of several transfer ribonucleic acid (RNA) species have been examined with particular emphasis on the extreme low-field portion. Betwen --13.8 and --15 ppm there are two extra resonances which are not derived from cloverleaf base pairs. A combined ap...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00585a025
更新日期：1979-09-04 00:00:00
abstract：:Human serum albumin (HSA) is the most abundant protein in the blood plasma and is involved in the transport of metal ions. Four metal-binding sites with different specificities have been described in HSA: (i) the N-terminal site provided by Asp1, Ala2, and His3, (ii) the site at the reduced Cys34, (iii) site A, includ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061783p
更新日期：2007-02-27 00:00:00
abstract：:The actinomycin biosynthetic gene cluster of Streptomyces chrysomallus harbors two paralogous genes, acmI and acmL, encoding methyltransferases. To unveil their suspected role in the formation of 3-hydroxy-4-methyl-anthranilic acid (4-MHA), the building block of the actinomycin chromophore, each gene was expressed in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101422r
更新日期：2010-11-16 00:00:00
abstract：:Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00168a015
更新日期：1994-01-18 00:00:00
abstract：:Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2016537
更新日期：2012-02-07 00:00:00
abstract：:The human endonuclease III (hNTH1) is an important DNA glycosylase with associated abasic lyase activity. We previously demonstrated that the K212Q mutant was totally inactive, while the K212R mutant had reduced DNA glycosylase/lyase activity and could form a covalent complex with the substrate DNA upon reduction. We ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011053b
更新日期：2001-11-13 00:00:00
abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01298
更新日期：2019-03-05 00:00:00
abstract：:The murine urokinase-type plasminogen activator (uPA) gene has been isolated from a BALB/c liver DNA cosmid library and its nucleotide sequence established. The gene is organized into 11 exons comprising 34.7% of the 6710 base pair (bp) region spanning the interval between the presumed transcription initiation and pol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00399a038
更新日期：1987-12-15 00:00:00
abstract：:The oxidation of alcohol to aldehyde by horse liver alcohol dehydrogenase (LADH) requires the transfer of a hydride ion from the alcohol substrate to the cofactor nicotinamide adenine dinucleotide (NAD). A quantum mechanical tunneling contribution to this hydride transfer step has been demonstrated in a number of LADH...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973184b
更新日期：1998-06-30 00:00:00
abstract：:DBA/2 mouse spleen cells were stimulated in vitro by (a) alloantigen (mitomycin-treated CBA/J splenocytes), (b) the T cell mitogen concanavalin A (Con A), and (c) the B cell mitogen lipopolysaccharide (LPS). The cultures were pulsed for 10 h with (14)C-labeled galactose and glucosamine. Radiolabeled glycosphingolipids...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00519a002
更新日期：1981-08-04 00:00:00
abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.6b00703
更新日期：2016-09-27 00:00:00
abstract：:One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700878g
更新日期：2007-09-04 00:00:00
abstract：:Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00233a022
更新日期：1991-05-14 00:00:00
abstract：:Vanadate can often bind to phosphoryl transfer enzymes to form a trigonal-bipyramidal structure at the active site. The enzyme-vanadate dissociation constants in these enzymes are much lower than those for phosphate. Therefore, enzyme-bound vanadate moieties are often considered as transition state analogues. To test ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi016097z
更新日期：2002-05-07 00:00:00
abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00875
更新日期：2015-10-20 00:00:00
abstract：:We investigated the effects of substituting two of the four tryptophans (the "inner pair" Trp(9) and Trp(11) or the "outer pair" Trp(13) and Trp(15)) in gramicidin A (gA) channels. The conformational preferences of the doubly substituted gA analogues were assessed using circular dichroism spectroscopy and size-exclusi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200136e
更新日期：2011-06-07 00:00:00
abstract：:We have determined the three-dimensional structure of a non-self-complementary oligodeoxynucleotide duplex that contains a model abasic site. The duplex contains six GC base pairs plus the abasic site at the center of one strand and corresponds to an abasic frameshift. Two-dimensional NMR studies on the nonexchangeabl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00431a009
更新日期：1989-03-07 00:00:00
abstract：:CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin's ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000995y
更新日期：2000-10-17 00:00:00
abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011384o
更新日期：2001-12-18 00:00:00
abstract：:Alignment of more than 20 deduced sequences for mevalonate diphosphate decarboxylase (MDD) indicates that serines 34, 36, 120,121, 153, and 155 are invariant residues that map within a proposed interdomain active site cleft. To test possible active site roles for these invariant serines, each has been mutated to alani...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0484217
更新日期：2005-02-22 00:00:00
abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900374c
更新日期：2009-06-16 00:00:00
abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0267994
更新日期：2003-02-18 00:00:00
abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971760y
更新日期：1997-12-02 00:00:00
abstract：:In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01063
更新日期：2017-03-21 00:00:00
