Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha.


:Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and mapped H-NS binding sites. Cnu consists of three alpha helices that are comparable with those of Hha, but it has significant flexibility in the C-terminal region and lacks a short alpha helix present in Hha. Upon increasing ionic strength, the helical structure of Cnu is destabilized, especially at the ends of the helices. The dominant H-NS binding sites, located at helix 3 as in Hha, reveal a common structural platform for H-NS binding. Our results may provide structural and dynamic bases for the similarity and dissimilarity between Cnu and Hha functions.






Bae SH,Liu D,Lim HM,Lee Y,Choi BS




Has Abstract


2008-02-19 00:00:00












  • Single-turnover kinetics of helicase-catalyzed DNA unwinding monitored continuously by fluorescence energy transfer.

    abstract::We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...


    pub_type: 杂志文章


    authors: Bjornson KP,Amaratunga M,Moore KJ,Lohman TM

    更新日期:1994-11-29 00:00:00

  • Iso-mechanism of nitroalkane oxidase: 1. Inhibition studies and activation by imidazole.

    abstract::The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...


    pub_type: 杂志文章


    authors: Gadda G,Fitzpatrick PF

    更新日期:2000-02-15 00:00:00

  • Cooperative disulfide bond formation in apamin.

    abstract::Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...


    pub_type: 杂志文章


    authors: Chau MH,Nelson JW

    更新日期:1992-05-12 00:00:00

  • The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).

    abstract::Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...


    pub_type: 杂志文章


    authors: Gopal B,Madan LL,Betz SF,Kossiakoff AA

    更新日期:2005-01-11 00:00:00

  • Methyl-Based NMR Spectroscopy Methods for Uncovering Structural Dynamics in Large Proteins and Protein Complexes.

    abstract::NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...


    pub_type: 杂志文章,评审


    authors: Boswell ZK,Latham MP

    更新日期:2019-01-22 00:00:00

  • Hydrophobic core of the steroidogenic acute regulatory protein for cholesterol transport.

    abstract::The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...


    pub_type: 杂志文章


    authors: Bose HS,Whittal RM,Bose M,Debnath D

    更新日期:2009-02-17 00:00:00

  • Acylation of myelin basic protein peptide 1-21 with alkyl carboxylates 2-10 carbons long affects secondary structure and posttranslational modification.

    abstract::A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the seconda...


    pub_type: 杂志文章


    authors: Costentino M,Pritzker L,Boulias C,Moscarello MA

    更新日期:1994-04-12 00:00:00

  • Mechanism of action of phalloidin on the polymerization of muscle actin.

    abstract::Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical ac...


    pub_type: 杂志文章


    authors: Estes JE,Selden LA,Gershman LC

    更新日期:1981-02-17 00:00:00

  • Quantification of cysteinyl S-nitrosylation by fluorescence in unbiased proteomic studies.

    abstract::Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...


    pub_type: 杂志文章


    authors: Wiktorowicz JE,Stafford S,Rea H,Urvil P,Soman K,Kurosky A,Perez-Polo JR,Savidge TC

    更新日期:2011-06-28 00:00:00

  • Circular dichroism study of the interaction of glutamyl-tRNA synthetase with tRNAGlu2.

    abstract::The interaction of glutamyl-tRNA synthetase with tRNAGlu2 has been studied. The enzyme was purified to apparent homogeneity, and consists of a single chain with a molecular weight of 59 000. The sedimentation coefficient (sdegrees20,w) was found to be 3.7 S and suggests this enzyme is quite asymmetric. The enzyme bind...


    pub_type: 杂志文章


    authors: Willick GE,Kay CM

    更新日期:1976-09-21 00:00:00

  • Site-directed mutagenesis of a predicted cation binding site of Na, K-ATPase.

    abstract::Chemical modification and proteolytic digestion studies have identified a transmembrane glutamic acid residue (E953) of the alpha subunit of the pig kidney Na, K-ATPase as a possible cation binding site [Goldshleger et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6911-6915]. In addition, an adjacent glutamate (E954) i...


    pub_type: 杂志文章


    authors: Van Huysse JW,Jewell EA,Lingrel JB

    更新日期:1993-01-26 00:00:00

  • Identification of phosphorylation sites of TOPORS and a role for serine 98 in the regulation of ubiquitin but not SUMO E3 ligase activity.

    abstract::TOPORS is the first example of a protein that possesses both ubiquitin and SUMO E3 ligase activity. The ubiquitination activity maps to a conserved RING domain in the N-terminal region of the protein, which is not required for sumoylation activity. Similar to other E3 ligases, it is likely that the ubiquitin and sumoy...


    pub_type: 杂志文章


    authors: Park HJ,Zheng H,Kulkarni D,Kerrigan J,Pungaliya P,Saleem A,Rubin EH

    更新日期:2008-12-30 00:00:00

  • Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state.

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    pub_type: 杂志文章


    authors: van der Does C,Manting EH,Kaufmann A,Lutz M,Driessen AJ

    更新日期:1998-01-06 00:00:00

  • Picosecond infrared study of the photodynamics of carbonmonoxy-cytochrome c oxidase.

    abstract::Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...


    pub_type: 杂志文章


    authors: Dyer RB,Peterson KA,Stoutland PO,Woodruff WH

    更新日期:1994-01-18 00:00:00

  • Coenzyme Q analogues reconstitute electron transport and proton ejection but not the antimycin-induced "red shift" in mitochondria from coenzyme Q deficient mutants of the yeast Saccharomyces cerevisiae.

    abstract::Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB...


    pub_type: 杂志文章


    authors: Beattie DS,Clejan L

    更新日期:1986-03-25 00:00:00

  • Domain organization and functional analysis of type IIS restriction endonuclease Eco31I.

    abstract::Type IIS restriction endonuclease Eco31I harbors a single HNH active site and cleaves both DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). A two-domain organization of Eco31I was determined by limited proteolysis. Analysis of proteolytic fragments revealed that the N-terminal domain of Eco31I is respons...


    pub_type: 杂志文章


    authors: Jakubauskas A,Sasnauskas G,Giedriene J,Janulaitis A

    更新日期:2008-08-19 00:00:00

  • Solution structure of decorin-binding protein A from Borrelia burgdorferi.

    abstract::Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...


    pub_type: 杂志文章


    authors: Wang X

    更新日期:2012-10-23 00:00:00

  • Refinement of netropsin bound to DNA: bias and feedback in electron density map interpretation.

    abstract::The X-ray crystal structure of the complex of the B-DNA dodecamer CGCGAATTCGCG with the antitumor drug netropsin has been reexamined to locate the drug accurately for computer-based drug design. The optimum solution is with the drug centered in the AATT region of the minor groove, making three good bifurcated hydrogen...


    pub_type: 杂志文章


    authors: Goodsell DS,Kopka ML,Dickerson RE

    更新日期:1995-04-18 00:00:00

  • Synthesis of lysogangliosides.

    abstract::The synthesis of gangliosides GM3, GM2, GM1, and GD1a solely lacking the fatty acid moiety, and thus called lysogangliosides in analogy to lysophospholipids, is described. Since a selective elimination of the fatty acid residue has not been achieved as yet, the gangliosides were first subjected to alkaline hydrolysis....


    pub_type: 杂志文章


    authors: Neuenhofer S,Schwarzmann G,Egge H,Sandhoff K

    更新日期:1985-01-15 00:00:00

  • An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression.

    abstract::Kinases play a critical role in cellular signaling and are dysregulated in a number of diseases, such as cancer, diabetes, and neurodegeneration. Therapeutics targeting kinases currently account for roughly 50% of cancer drug discovery efforts. The ability to explore human kinase biochemistry and biophysics in the lab...


    pub_type: 杂志文章


    authors: Albanese SK,Parton DL,Işık M,Rodríguez-Laureano L,Hanson SM,Behr JM,Gradia S,Jeans C,Levinson NM,Seeliger MA,Chodera JD

    更新日期:2018-08-07 00:00:00

  • Exploring the Evolutionary History of Kinetic Stability in the α-Lytic Protease Family.

    abstract::In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...


    pub_type: 杂志文章


    authors: Nixon CF,Lim SA,Sailer ZR,Zheludev IN,Gee CL,Kelch BA,Harms MJ,Marqusee S

    更新日期:2021-01-26 00:00:00

  • FTIR study of the thermal denaturation of horseradish and cytochrome c peroxidases in D2O.

    abstract::Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...


    pub_type: 杂志文章


    authors: Holzbaur IE,English AM,Ismail AA

    更新日期:1996-04-30 00:00:00

  • Resonance Raman interrogation of the consequences of heme rotational disorder in myoglobin and its ligated derivatives.

    abstract::Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives, i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. The spectra for the reversed forms of these derivatives have been extracted from the spect...


    pub_type: 杂志文章


    authors: Rwere F,Mak PJ,Kincaid JR

    更新日期:2008-12-02 00:00:00

  • HO. and DNase I probing of E sigma 70 RNA polymerase--lambda PR promoter open complexes: Mg2+ binding and its structural consequences at the transcription start site.

    abstract::Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...


    pub_type: 杂志文章


    authors: Craig ML,Suh WC,Record MT Jr

    更新日期:1995-12-05 00:00:00

  • Photoreactivities and thermal properties of psoralen cross-links.

    abstract::We have studied the photoreaction of 8-methoxypsoralen (8-MOP), 4,5',8-trimethylpsoralen (TMP), and 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with a pair of 18-base-long oligonucleotides in which a 14-base region is complementary. Only one 5'TpA site, favored for both monoadduct and cross-link formation with p...


    pub_type: 杂志文章


    authors: Yeung AT,Jones BK,Chu CT

    更新日期:1988-05-03 00:00:00

  • Limited proteolysis of human growth hormone at low pH: isolation, characterization, and complementation of the two biologically relevant fragments 1-44 and 45-191.

    abstract::The limited proteolysis approach was used to analyze the conformational features of human growth hormone (hGH) under acidic solvent conditions (A-state). Pepsin was used as the proteolytic probe because of its poor substrate specificity and its activity at low pH. Limited proteolysis of hGH in its A-state results in a...


    pub_type: 杂志文章


    authors: Spolaore B,Polverino de Laureto P,Zambonin M,Fontana A

    更新日期:2004-06-01 00:00:00

  • Fourier-transform infrared spectroscopy of phytochrome: difference spectra of the intermediates of the photoreactions.

    abstract::The photocycle of 124 kDa phytochrome A from Avena sativa was studied by Fourier-transform infrared spectroscopy at low temperatures. Difference spectra between the parent state Pr and the intermediates of the Pr-->Pfr pathway, i.e. lumi-R, meta-Ra, and meta-Rc, and between Pfr and the intermediates of the Pfr-->Pr pa...


    pub_type: 杂志文章


    authors: Foerstendorf H,Mummert E,Schäfer E,Scheer H,Siebert F

    更新日期:1996-08-20 00:00:00

  • Distance between the visible copper and cytochrome a in bovine heart cytochrome oxidase.

    abstract::Electron paramagnetic resonance (EPR) at 15 K was used to probe the magnetic interaction between the visible copper CuA2+ and ferric cytochrome a in the carbon monoxide compound of beef heart cytochrome oxidase. At pH 8.6, the midpoint potentials (Em's) for one-electron oxidation of CuA+ and cytochrome a2+ were found ...


    pub_type: 杂志文章


    authors: Goodman G,Leigh JS Jr

    更新日期:1985-04-23 00:00:00

  • Chemical modification of prostaglandin H synthase with diethyl pyrocarbonate.

    abstract::The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7...


    pub_type: 杂志文章


    authors: Zhang X,Tsai AL,Kulmacz RJ

    更新日期:1992-03-10 00:00:00

  • Examination of the solvent perturbation technique as a method to identify enzyme catalytic groups.

    abstract::The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...


    pub_type: 杂志文章


    authors: Grace S,Dunaway-Mariano D

    更新日期:1983-08-30 00:00:00