Characterization of a recombinant extracellular domain of the type 1 tumor necrosis factor receptor: evidence for tumor necrosis factor-alpha induced receptor aggregation.

abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...

journal_title：Biochemistry

authors： Holzbaur IE,English AM,Ismail AA

更新日期：1996-04-30 00:00:00

abstract：:A GTP:RNA guanylyltransferase or capping enzyme has been purified approximately 2000-fold from wheat germ. The enzyme catalyzes the transfer of the GMP residue from GTP to the 5' end of RNA or synthetic polyribonucleotides. Diphosphate-ended polymers were capped more efficiently than molecules with triphosphate ends, ...

journal_title：Biochemistry

authors： Keith JM,Venkatesan S,Gershowitz A,Moss B

更新日期：1982-01-19 00:00:00

abstract：:The conformational properties of the magainin family of antimicrobial peptides in aqueous solution and in model membranes have been probed by Fourier transform infrared spectroscopy. The magainins were found to be structureless in aqueous solution at neutral pD, confirming other studies by Raman and circular dichroism...

journal_title：Biochemistry

authors： Jackson M,Mantsch HH,Spencer JH

更新日期：1992-08-18 00:00:00

abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...

journal_title：Biochemistry

authors： van de Poll ML,van Vugt MJ,Lenferink AE,van Zoelen EJ

更新日期：1997-06-17 00:00:00

abstract：:The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...

journal_title：Biochemistry

authors： Alam S,Grum-Tokars V,Krucinska J,Kundracik ML,Wedekind JE

更新日期：2005-11-08 00:00:00

abstract：:The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-i...

journal_title：Biochemistry

authors： Jockel P,Schmid M,Choinowski T,Dimroth P

更新日期：2000-04-18 00:00:00

abstract：:We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...

journal_title：Biochemistry

authors： Buehler J,Qwan E,DeGregorio MW,Macher BA

更新日期：1985-11-19 00:00:00

abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

journal_title：Biochemistry

authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

更新日期：1995-12-05 00:00:00

abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

journal_title：Biochemistry

authors： Chen FM

更新日期：1984-12-04 00:00:00

abstract：:The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...

journal_title：Biochemistry

authors： Bahr BA,Clarkson ED,Rogers GA,Noremberg K,Parsons SM

更新日期：1992-06-30 00:00:00

abstract：:DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[ ]Pyr). In t...

journal_title：Biochemistry

authors： Kim ST,Malhotra K,Smith CA,Taylor JS,Sancar A

更新日期：1993-07-20 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...

journal_title：Biochemistry

authors： Anderson MS,Robertson AD,Macher I,Raetz CR

更新日期：1988-03-22 00:00:00

abstract：:A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

journal_title：Biochemistry

authors： Rad B,Kowalczykowski SC

更新日期：2012-04-03 00:00:00

abstract：:Triple helix-forming oligonucleotides (TFOs) bind with high affinity and specificity to homopurine-homopyrimidine sequences in DNA and have been shown to inhibit transcription of target genes in various experimental systems. In the present study, we evaluated the ability of 3'-amino-modified phosphodiester TFOs direct...

journal_title：Biochemistry

authors： Catapano CV,McGuffie EM,Pacheco D,Carbone GM

更新日期：2000-05-02 00:00:00

abstract：:Human prolactin (hPRL) binds two human prolactin receptor molecules, creating active heterotrimeric complexes. Receptors bind dissimilar hormone surfaces termed site 1 and site 2 in an obligate ordered process. We sought to map the functional epitopes in site 1 of hPRL. Extensive alanine mutagenesis (102 of the 199 re...

journal_title：Biochemistry

authors： Rao GV,Brooks CL

更新日期：2011-03-01 00:00:00

abstract：:The interactions of bovine brain MAP2 and tau, recombinant murine MAP2C, and recombinant human tau with phosphatidylinositol vesicles yield apparent Kd values of 51 +/- 6 nM, 2.4 +/- 0.6 microM, 1.4 +/- 0.1 microM, and 1.6 +/- 0.2 microM, respectively. Examinations of the binding of MAP2 and/or MAP2C to phosphatidylch...

journal_title：Biochemistry

authors： Surridge CD,Burns RG

更新日期：1994-07-05 00:00:00

abstract：:The structures of ternary complexes of human inositol monophosphatase with inhibitory Gd3+ and either D- or L-myo-inositol 1-phosphate have been determined to 2.2-2.3 A resolution using X-ray crystallography. Substrate and metal are bound identically in each active site of the phosphatase dimer. The substrate is prese...

journal_title：Biochemistry

authors： Bone R,Frank L,Springer JP,Pollack SJ,Osborne SA,Atack JR,Knowles MR,McAllister G,Ragan CI,Broughton HB

更新日期：1994-08-16 00:00:00

abstract：:We have investigated the microsecond rotational motions of the Ca-ATPase in rabbit skeletal sarcoplasmic reticulum (SR), by measuring the time-resolved phosphorescence anisotropy of erythrosin 5-isothiocyanate (ERITC) covalently and specifically attached to the enzyme. Over a wide range of solvent conditions and tempe...

journal_title：Biochemistry

authors： Birmachu W,Thomas DD

更新日期：1990-04-24 00:00:00

abstract：:Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...

journal_title：Biochemistry

authors： Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

更新日期：1999-07-13 00:00:00

abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

journal_title：Biochemistry

authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

更新日期：2010-05-18 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:Zinc finger nucleases (ZFNs) have been used to direct precise modifications of the genetic information in living cells at high efficiency. An important consideration in the design of ZFNs is the number of zinc fingers that are required for efficient and specific cleavage. We examined dimeric ZFNs composed of [1]+[1], ...

journal_title：Biochemistry

authors： Shimizu Y,Şöllü C,Meckler JF,Adriaenssens A,Zykovich A,Cathomen T,Segal DJ

更新日期：2011-06-07 00:00:00

abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...

journal_title：Biochemistry

authors： Clausen H,McKibbin JM,Hakomori S

更新日期：1985-10-22 00:00:00

abstract：:Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent di...

journal_title：Biochemistry

authors： Gasselhuber B,Graf MM,Jakopitsch C,Zamocky M,Nicolussi A,Furtmüller PG,Oostenbrink C,Carpena X,Obinger C

更新日期：2016-06-28 00:00:00

abstract：:Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from bot...

journal_title：Biochemistry

authors： Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

更新日期：2014-08-19 00:00:00

abstract：:Fluorescent derivatives of short oligonucleotides of defined sequence were prepared by the incorporation of 5-(propylamino)uridine via current phosphoramidite chemistry, followed by derivatization of the propylamine function with mansyl chloride. These oligomers, annealed to complementary oligomers, yielded short dupl...

journal_title：Biochemistry

authors： Allen DJ,Darke PL,Benkovic SJ

更新日期：1989-05-30 00:00:00

abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...

journal_title：Biochemistry

authors： Breton J

更新日期：2007-04-17 00:00:00

abstract：:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone involved in the maintenance of the corpus luteum in early pregnancy. The free alpha-subunit of hCG has a biological activity of its own, namely, stimulation of prolactin secretion from term pregnancy decidual cells [Blithe, D. L., et al. (1991)...

journal_title：Biochemistry

authors： van Zuylen CW,de Beer T,Leeflang BR,Boelens R,Kaptein R,Kamerling JP,Vliegenthart JF

更新日期：1998-02-17 00:00:00