DNA photolyase repairs the trans-syn cyclobutane thymine dimer.

abstract：:12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even aft...

journal_title：Biochemistry

authors： Rotenberg SA,Grandizio AM,Selzer AT,Clapp CH

更新日期：1988-11-29 00:00:00

abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

journal_title：Biochemistry

authors： Yang XY,Schulz H,Elzinga M,Yang SY

更新日期：1991-07-09 00:00:00

abstract：:The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...

journal_title：Biochemistry

authors： Yanagisawa S,Sato K,Kikuchi M,Kohzuma T,Dennison C

更新日期：2003-06-10 00:00:00

abstract：:The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...

journal_title：Biochemistry

authors： Phillips RS,Sundararaju B,Koushik SV

更新日期：1998-06-16 00:00:00

abstract：:The influence of DNA base sequence context on the removal of a bulky benzo[a]pyrene diol epoxide-guanine adduct, (+)-trans-B[a]P-N2-dG (G*), by UvrABC nuclease from the thermophilic organism Bacillus caldotenax was investigated. The lesion was flanked by either T or C in otherwise identical complementary 43-mer duplex...

journal_title：Biochemistry

authors： Ruan Q,Liu T,Kolbanovskiy A,Liu Y,Ren J,Skorvaga M,Zou Y,Lader J,Malkani B,Amin S,Van Houten B,Geacintov NE

更新日期：2007-06-12 00:00:00

abstract：:An NMR and nuclear Overhauser effect (NOE) analysis of Thermus thermophilus tRNAIle1a is presented. This species contains modifications including s2T54 and s4U8 [Horie, N., Hara-Yokoyama, M., Yokoyama, S., Watanabe, K., Kuchino, Y., Nishimura, S., & Miyazawa, T. (1985) Biochemistry 24, 5711-5715]. All the expected sec...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1986-04-08 00:00:00

abstract：:Curcumin potentiates the phosphorylation-dependent activity of human cystic fibrosis transmembrane conductance regulator (CFTR) in Fe(3+)-dependent and Fe(3+)-independent manners. Although the Fe(3+)-dependent curcumin potentiation results from removal of endogenous inhibitory Fe(3+) at the interface of the regulatory...

journal_title：Biochemistry

authors： Wang G

更新日期：2015-05-12 00:00:00

abstract：:The human acid sphingomyelinase (ASM, EC 3.1.4.12), a lysosomal and secretory protein coded by the sphingomyelin phosphodiesterase 1 (SMPD-1) gene, catalyzes the degradation of sphingomyelin (SM) to ceramide and phosphorylcholine. We examined the structural-functional properties of its carboxyl-terminus (amino acids 4...

journal_title：Biochemistry

authors： Lee CY,Tamura T,Rabah N,Lee DY,Ruel I,Hafiane A,Iatan I,Nyholt D,Laporte F,Lazure C,Wada I,Krimbou L,Genest J

更新日期：2007-12-25 00:00:00

abstract：:Intracellular retinoid-binding proteins are small, tightly folded, compact proteins, which appear to be involved in the delivery of retinoids to microsomal metabolic enzymes, among other potential roles. Recently, it has been demonstrated that two of these binding proteins, cellular retinol-binding protein (CRBP) and ...

journal_title：Biochemistry

authors： Jamison RS,Newcomer ME,Ong DE

更新日期：1994-03-15 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:Thermal unfolding/refolding studies of the three tropomyosin dimers, alpha alpha, alpha beta, and beta beta, from chicken gizzard muscle were performed to explain the preferential assembly of alpha- and beta-tropomyosin subunits into heterodimers, alpha beta [Lehrer, S. S., & Qian, Y. (1989) J. Biol. Chem. 265, 1134]....

journal_title：Biochemistry

authors： Lehrer SS,Stafford WF 3rd

更新日期：1991-06-11 00:00:00

abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...

journal_title：Biochemistry

authors： Lee RT,Myers RW,Lee YC

更新日期：1982-11-23 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:Synthetic photocontrolled proteins could be powerful tools for probing cellular chemistry. Several previous attempts to produce such systems by incorporating photoisomerizable chromophores into biomolecules have led to photocontrol but with incomplete reversibility, where the chromophore becomes trapped in one photois...

journal_title：Biochemistry

authors： Woolley GA,Jaikaran AS,Berezovski M,Calarco JP,Krylov SN,Smart OS,Kumita JR

更新日期：2006-05-16 00:00:00

abstract：:In bacteria, two-component systems act as signaling systems to respond to environmental stimuli. Two-component systems generally consist of a sensor histidine kinase and a response regulator, which work together through histidyl-aspartyl phosphorelay to result in gene regulation. One of the two-component systems in Es...

journal_title：Biochemistry

authors： Affandi T,Issaian AV,McEvoy MM

更新日期：2016-09-20 00:00:00

abstract：:Traditional antibody generation, using either phage display or animal immunization, relies on purified antigens. Many membrane proteins, such as G protein-coupled receptors, solute carriers, or ion channels, are important drug targets but very challenging for the formation of antibodies due to the difficulty of protei...

journal_title：Biochemistry

authors： Chang YC,Kao CY,Tang HC,Huang MS,Mou KY

更新日期：2020-11-10 00:00:00

abstract：:Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...

journal_title：Biochemistry

authors： Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

更新日期：2005-11-29 00:00:00

abstract：:The effects of mutation of the conserved serine92 residue to alanine, valine, and leucine in pig myoglobin have been determined. In myoglobin crystal structures, the hydroxyl group of serine92 is within hydrogen-bonding distance of the N delta-H of histidine93, whose N epsilon coordinates the iron atom of the heme pro...

journal_title：Biochemistry

authors： Smerdon SJ,Krzywda S,Wilkinson AJ,Brantley RE Jr,Carver TE,Hargrove MS,Olson JS

更新日期：1993-05-18 00:00:00

abstract：:In two recent papers, we reported the effects of several point mutations on the thermodynamics of the thermal unfolding of the lysozyme of phage T4 as determined by differential scanning calorimetry. The mutants studied were R96H [Kitamura, S., & Sturtevant, J.M. (1989) Biochemistry 28, 3788-3792] and T157 replaced by...

journal_title：Biochemistry

authors： Hu CQ,Kitamura S,Tanaka A,Sturtevant JM

更新日期：1992-02-18 00:00:00

abstract：:The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We ...

journal_title：Biochemistry

authors： van der Horst MA,van Stokkum IH,Dencher NA,Hellingwerf KJ

更新日期：2005-06-28 00:00:00

abstract：:Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5'-diphosphates (NDPs) to deoxynucleotides (dNDPs). The active site for NDP reduction resides in α2, and the essential diferric-tyrosyl radical (Y(122)(•)) cofactor that initiates transfer of the radical to the act...

journal_title：Biochemistry

authors： Seyedsayamdost MR,Yee CS,Stubbe J

更新日期：2011-03-01 00:00:00

abstract：:A method has been developed for the routine synthesis of 2'(3')-o-monoacyl ribonucleoside 5'-diphosphates for stepwise synthesis of oligoribonucleotides with Escherichia coli polynucleotide phosphorylase. The use of triethyl orthoisovalerate allows the facile preparation of 2'(3')-o-isovaleryl-UDP, -CDP, -ADP, -GDP, -...

journal_title：Biochemistry

authors： Walker GC,Uhlenbeck OC

更新日期：1975-02-25 00:00:00

abstract：:Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured by electron spin resonance, utilizing fatty acid spin probes. The use of such probes is controver...

journal_title：Biochemistry

authors： Taraschi TF,Wu A,Rubin E

更新日期：1985-12-03 00:00:00

abstract：:Myosin belongs to the family of motor proteins. Its interaction with actin coupled with hydrolysis of ATP is the molecular basis of muscle contraction. The head segment of myosin, called subfragment 1 (S1), contains the distinct binding sites for ATP and actin and responsible for the ATPase activity. The rate-limiting...

journal_title：Biochemistry

authors： Peyser YM,Ben-Hur M,Werber MM,Muhlrad A

更新日期：1996-04-09 00:00:00

abstract：:High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

journal_title：Biochemistry

authors： Heer A,Alonso LG,de Prat-Gay G

更新日期：2011-03-01 00:00:00

abstract：:S-Adenosylmethionine decarboxylase is a pyruvate-dependent enzyme. The enzyme forms a Schiff base with substrate, S-adenosylmethionine, through the pyruvoyl moiety. This facilitates the release of CO2 from the substrate, which must then be protonated on the alpha carbon in order to permit hydrolysis of the Schiff base...

journal_title：Biochemistry

authors： Xiong H,Stanley BA,Pegg AE

更新日期：1999-02-23 00:00:00

abstract：:Helicases unwind double-stranded nucleic acids, remove secondary structures from single-stranded nucleic acids, and remove proteins bound to nucleic acids. For many helicases, the mechanisms for these different functions share the ability to translocate with a directional bias as a result of ATP binding and hydrolysis...

journal_title：Biochemistry

authors： Reynolds KA,Cameron CE,Raney KD

更新日期：2015-07-14 00:00:00

abstract：:A 31 amino acid fragment of the extracellular N-terminus of the human G-protein coupled receptor for parathyroid hormone (PTH1R) has been structurally characterized by NMR and molecular dynamics simulations. The fragment PTH1R[168-198] includes residues 173-189, shown by photoaffinity cross-linking to be a contact dom...

journal_title：Biochemistry

authors： Pellegrini M,Bisello A,Rosenblatt M,Chorev M,Mierke DF

更新日期：1998-09-15 00:00:00

abstract：:The three-dimensional solution structure of toxin FS2, a 60-residue polypeptide isolated from the venom of black mamba snake (Dendroaspis polylepis polylepis), has been determined by nuclear magnetic resonance spectroscopy. Using 600 NOE constraints and 55 dihedral angle constraints, a set of 20 structures obtained fr...

journal_title：Biochemistry

authors： Albrand JP,Blackledge MJ,Pascaud F,Hollecker M,Marion D

更新日期：1995-05-02 00:00:00

abstract：:The conformation and activity of three different lipases have been studied in reverse micelles formed by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. In the case of human pancreatic lipase, the conformation of the polypeptide chain--as judged from far-UV circular dichroism measurements--is only slightly...

journal_title：Biochemistry

authors： Walde P,Han D,Luisi PL

更新日期：1993-04-20 00:00:00