Reversible photocontrol of DNA binding by a designed GCN4-bZIP protein.

abstract：:Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...

journal_title：Biochemistry

authors： LeBlanc DA,Morden KM

更新日期：1991-04-23 00:00:00

abstract：:One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...

journal_title：Biochemistry

authors： Eckel LM,Krugh TR

更新日期：1994-11-22 00:00:00

abstract：:We recently introduced a new substrate rescue tool for investigating enzymatic base flipping by uracil DNA glycosylase (UDG) in which a bulky pyrene nucleotide wedge (Y) was placed opposite a uracil in duplex DNA (i.e., a U/Y pair), thereby preorganizing the target base in an extrahelical conformation [Jiang, Y. L., e...

journal_title：Biochemistry

authors： Jiang YL,Stivers JT,Song F

更新日期：2002-09-17 00:00:00

abstract：:The crystal structure of the recombinant thiamin diphosphate-dependent E1 component from the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) has been determined at a resolution of 1.85 A. The E. coli PDHc E1 component E1p is a homodimeric enzyme and crystallizes with an intact dimer in an asymmetric...

journal_title：Biochemistry

authors： Arjunan P,Nemeria N,Brunskill A,Chandrasekhar K,Sax M,Yan Y,Jordan F,Guest JR,Furey W

更新日期：2002-04-23 00:00:00

abstract：:The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the s...

journal_title：Biochemistry

authors： Dhingra MM,Sarma RH,Uesugi S,Shida T,Ikehara M

更新日期：1981-08-18 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Heme catabolism is an important biochemical process that many bacterial pathogens utilize to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further processing for transport out of the cell or conversion to another useful cofactor. In previous work, we presented in vitro evidence of a...

journal_title：Biochemistry

authors： LaMattina JW,Delrossi M,Uy KG,Keul ND,Nix DB,Neelam AR,Lanzilotta WN

更新日期：2017-02-14 00:00:00

abstract：:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...

journal_title：Biochemistry

authors： Seela F,Tran TQ,Mentzel H,Erdmann VA

更新日期：1981-04-28 00:00:00

abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...

journal_title：Biochemistry

authors： Shatzky-Schwartz M,Hiller Y,Reich Z,Ghirlando R,Weinberger S,Minsky A

更新日期：1992-03-03 00:00:00

abstract：:An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expressed in Escherichia coli and purified to homogeneity. Purified (Nif)IscA was found to be a homodimer of 11-kDa subunits that contained no metal centers or other prosthetic groups in its as-isolated form. Possible roles f...

journal_title：Biochemistry

authors： Krebs C,Agar JN,Smith AD,Frazzon J,Dean DR,Huynh BH,Johnson MK

更新日期：2001-11-20 00:00:00

abstract：:The characteristic period four oscillation patterns of oxygen evolution induced by a train of single-turnover flashes were measured as a function of temperature in dark-adapted photosystem II (PS II) membrane fragments that were reconstituted with native plastoquinone-9 (PQ-9) by a recently developed procedure [Kurrec...

journal_title：Biochemistry

authors： Seeliger AG,Kurreck J,Renger G

更新日期：1997-03-04 00:00:00

abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

journal_title：Biochemistry

authors： Sheardy RD,Winkle SA

更新日期：1989-01-24 00:00:00

abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

journal_title：Biochemistry

authors： Cloherty EK,Levine KB,Carruthers A

更新日期：2001-12-25 00:00:00

abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...

journal_title：Biochemistry

authors： Watson J,Nilsen-Hamilton M,Hamilton RT

更新日期：1976-04-06 00:00:00

abstract：:Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dit...

journal_title：Biochemistry

authors： Vanin EF,Ji TH

更新日期：1981-11-24 00:00:00

abstract：:Mistic is an unusual membrane protein from Bacillus subtilis. It appears to fold and insert autonomously into a lipid bilayer and has been suggested as a tool that aids the targeting of eukaryotic membrane proteins to bacterial membranes. The NMR structure of Mistic in detergent (LDAO) micelles has revealed it to be a...

journal_title：Biochemistry

authors： Psachoulia E,Bond PJ,Sansom MS

更新日期：2006-08-01 00:00:00

abstract：:A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...

journal_title：Biochemistry

authors： Wu M,Arimura GK,Yunis AA

更新日期：1977-05-03 00:00:00

abstract：:We examined the effects of water-soluble polymers of various degrees of hydrophobicity on the folding and aggregation of proteins. The polymers we chose were polyethylene glycol (PEG) and UCON (1:1 copolymer of ethylene glycol and propylene glycol). The presence of additional methyl groups in UCON makes it more hydrop...

journal_title：Biochemistry

authors： Breydo L,Sales AE,Frege T,Howell MC,Zaslavsky BY,Uversky VN

更新日期：2015-05-19 00:00:00

abstract：:Manganese peroxidase (MnP) is an extracellular heme enzyme that catalyzes the peroxide-dependent oxidation of Mn(II) to Mn(III). The Mn(III) is released from the enzyme in complex with oxalate. One heme propionate and the side chains of Glu35, Glu39, and Asp179 were identified as Mn(II) ligands in the 2.0 A resolution...

journal_title：Biochemistry

authors： Sundaramoorthy M,Youngs HL,Gold MH,Poulos TL

更新日期：2005-05-03 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

journal_title：Biochemistry

authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

更新日期：2007-05-22 00:00:00

abstract：:The orientation of eukaryotic cytochromes P450, with respect to the membrane of the endoplasmic reticulum, has been investigated. There is now good evidence that the tertiary structure of these proteins is essentially the same as that of the soluble bacterial isoenzyme cytochrome P450CI, with the exception of an exten...

journal_title：Biochemistry

authors： Edwards RJ,Murray BP,Singleton AM,Boobis AR

更新日期：1991-01-08 00:00:00

abstract：:The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...

journal_title：Biochemistry

authors： Wu G,Miller KW

更新日期：1994-08-09 00:00:00

abstract：:Thermal unfolding/refolding studies of the three tropomyosin dimers, alpha alpha, alpha beta, and beta beta, from chicken gizzard muscle were performed to explain the preferential assembly of alpha- and beta-tropomyosin subunits into heterodimers, alpha beta [Lehrer, S. S., & Qian, Y. (1989) J. Biol. Chem. 265, 1134]....

journal_title：Biochemistry

authors： Lehrer SS,Stafford WF 3rd

更新日期：1991-06-11 00:00:00

abstract：:The FTIR spectra for alkyl isocyanides (CNRs) change from a single nu(CN) band centered at approximately 2175 cm(-1) to two peaks at approximately 2075 and approximately 2125 cm(-1) upon binding to sperm whale myoglobin (Mb). The low- and high-frequency peaks have been assigned to in and out conformations, respectivel...

journal_title：Biochemistry

authors： Blouin GC,Olson JS

更新日期：2010-06-22 00:00:00

abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

journal_title：Biochemistry

authors： Rao DK,Kaur P

更新日期：2008-03-04 00:00:00

abstract：:The characterization of cod somatolactin (SL), a new pituitary protein belonging to the growth hormone/prolactin family, is described. Cod SL has a molecular weight of 26 kDa and consists of 209 amino acids, of which eight are Cys. The protein has three disulfide bonds between residues Cys5-Cys15, Cys65-Cys181, and Cy...

journal_title：Biochemistry

authors： Rand-Weaver M,Noso T,Muramoto K,Kawauchi H

更新日期：1991-02-12 00:00:00

abstract：:Subunits located near the cardiolipin binding sites of bovine heart cytochrome c oxidase (CcO) were identified by photolabeling with arylazido-cardiolipin analogues and detecting labeled subunits by reversed-phase HPLC and HPLC-electrospray ionization mass spectrometry. Two arylazido-containing cardiolipin analogues w...

journal_title：Biochemistry

authors： Sedlák E,Panda M,Dale MP,Weintraub ST,Robinson NC

更新日期：2006-01-24 00:00:00

abstract：:Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside...

journal_title：Biochemistry

authors： Freier SM,Tinoco I Jr

更新日期：1975-07-29 00:00:00

abstract：:During the past decade, there has been much debate about the enormous catalytic rate enhancement observed in enzymatic reactions involving carbanion intermediates. Our recent theoretical study has demonstrated the importance of the short strong hydrogen bond (SSHB) in the enzymatic reactions. Nevertheless, other recen...

journal_title：Biochemistry

authors： Kim KS,Kim D,Lee JY,Tarakeshwar P,Oh KS

更新日期：2002-04-23 00:00:00