The red blood cell glucose transporter presents multiple, nucleotide-sensitive sugar exit sites.

abstract：:Genetic studies have previously demonstrated that the Saccharomyces cerevisiae CDC9 gene product, which is functionally homologous to mammalian DNA ligase I, is required for DNA replication and is also involved in DNA repair and genetic recombination. In the present study we have purified the yeast enzyme. When measur...

journal_title：Biochemistry

authors： Tomkinson AE,Tappe NJ,Friedberg EC

更新日期：1992-12-01 00:00:00

abstract：:Despite a lack of convincing in vitro evidence and a number of sound refutations, it is widely accepted that renalase is an enzyme unique to animals that catalyzes the oxidative degradation of catecholamines in blood in order to lower vascular tone. Very recently, we identified isomers of β-NAD(P)H as substrates for r...

journal_title：Biochemistry

authors： Hoag MR,Roman J,Beaupre BA,Silvaggi NR,Moran GR

更新日期：2015-06-23 00:00:00

abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...

journal_title：Biochemistry

authors： Moore RA,Hayes SF,Fischer ER,Priola SA

更新日期：2007-06-19 00:00:00

abstract：:Pectate lyase C (pelC) was the first protein in which the parallel beta-helix structure was recognized. The unique features of parallel beta-helix-containing proteins-a relatively simple topology and unusual interactions among side chains-make pelC an interesting protein to study with respect to protein folding. In th...

journal_title：Biochemistry

authors： Kamen DE,Griko Y,Woody RW

更新日期：2000-12-26 00:00:00

abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

journal_title：Biochemistry

authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

更新日期：1995-09-12 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

journal_title：Biochemistry

authors： Gursky O,Mei X,Atkinson D

更新日期：2012-01-10 00:00:00

abstract：:Sequence-specific assignments of 1H NMR resonances are obtained for the backbone protons of Escherichia coli acyl carrier protein, acylated with an eight-carbon chain covalently attached to the prosthetic group thiol (octanoyl-ACP). Comparison of 1H-1H sequential connectivities in the NOESY spectra of octanoyl-ACP and...

journal_title：Biochemistry

authors： Jones PJ,Holak TA,Prestegard JH

更新日期：1987-06-16 00:00:00

abstract：:Peptide-membrane interactions are important for understanding the binding, partitioning, and folding of membrane proteins; the activity of antimicrobial and fusion peptides; and a number of other processes. We describe molecular dynamics simulations (10-25 ns) of two pentapeptides Ace-WLXLL (with X = Arg or Lys side c...

journal_title：Biochemistry

authors： Aliste MP,MacCallum JL,Tieleman DP

更新日期：2003-08-05 00:00:00

abstract：:Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to...

journal_title：Biochemistry

authors： Hays FA,Vargason JM,Ho PS

更新日期：2003-08-19 00:00:00

abstract：:Retinoschisin (RS1) is a retina-specific secreted protein encoding a conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in the X-linked retinoschisis gene (RS1) cause X-linked reti...

journal_title：Biochemistry

authors： Kotova S,Vijayasarathy C,Dimitriadis EK,Ikonomou L,Jaffe H,Sieving PA

更新日期：2010-08-24 00:00:00

abstract：:Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...

journal_title：Biochemistry

authors： Robinson GW

更新日期：1975-08-12 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:Tau fibrillization is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. Small molecules capable of both inhibiting aggregation and promoting filament disaggregation have been discovered, but knowledge of their mechanism of action and potential for testing in biological models is frag...

journal_title：Biochemistry

authors： Necula M,Chirita CN,Kuret J

更新日期：2005-08-02 00:00:00

abstract：:Electron transfer in reaction center core (RCC) complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum was studied by measuring flash-induced absorbance changes. The first preparation contained approximately three iron-sulfur centers, indicating that the three putative electron acce...

journal_title：Biochemistry

authors： Schmidt KA,Neerken S,Permentier HP,Hager-Braun C,Amesz J

更新日期：2000-06-20 00:00:00

abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...

journal_title：Biochemistry

authors： Pates RD,Marsh D

更新日期：1987-01-13 00:00:00

abstract：:Respiratory complex I couples the electron transfer exclusively from NADH to a quinone with the translocation of protons across the membrane. However, Escherichia coli adapts to imposed high cellular NADPH concentrations by selecting the mutations E183A(F) and E183G(F) that lead to a high catalytic efficiency of compl...

journal_title：Biochemistry

authors： Frick K,Schulte M,Friedrich T

更新日期：2015-05-12 00:00:00

abstract：:Respiration, photosynthesis, and metabolism require the transfer of electrons through and between proteins over relatively long distances. It is critical that this electron transfer (ET) occur with specificity to avoid cellular damage, and at a rate that is sufficient to support the biological activity. A multistep ho...

journal_title：Biochemistry

authors： Choi M,Shin S,Davidson VL

更新日期：2012-09-04 00:00:00

abstract：:A specific tertiary hydrogen bond that is present between the side-chain hydroxyl group of Tyr30 and the side-chain N delta 1 atom of His17 in the small, monomeric, single-domain protein, barstar, has been perturbed by site-directed mutagenesis of the sole histidine residue (His17) to a glutamine residue. The effect o...

journal_title：Biochemistry

authors： Nath U,Udgaonkar JB

更新日期：1995-02-07 00:00:00

abstract：:The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule Q(B) to Q(B)H(2). In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknow...

journal_title：Biochemistry

authors： Paddock ML,Adelroth P,Feher G,Okamura MY,Beatty JT

更新日期：2002-12-17 00:00:00

abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...

journal_title：Biochemistry

authors： Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

更新日期：2016-07-26 00:00:00

abstract：:Proton-detected heteronuclear multiple-bond 1H-13C correlations (HMBC) previously have been used for assignment purposes in a variety of isotopically enriched proteins. In the present study it is demonstrated that the technique yields an almost complete assignment of the natural abundance 13C spectrum of the protein b...

journal_title：Biochemistry

authors： Hansen PE

更新日期：1991-10-29 00:00:00

abstract：:The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...

journal_title：Biochemistry

authors： Bann JG,Frieden C

更新日期：2004-11-02 00:00:00

abstract：:RosB catalyzes the formation of 8-aminoriboflavin 5'-phosphate (AFP), the key intermediate in roseoflavin biosynthesis, from the metabolic precursors riboflavin 5'-phosphate (RP, also known as FMN) and glutamate. The conversion of the aromatic methyl group at position 8 in RP into the aromatic amine in AFP occurs via ...

journal_title：Biochemistry

authors： Kapoor I,Nair SK

更新日期：2018-09-11 00:00:00

abstract：:The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regul...

journal_title：Biochemistry

authors： Auclair JR,Brodkin HR,D'Aquino JA,Petsko GA,Ringe D,Agar JN

更新日期：2013-09-10 00:00:00

abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

journal_title：Biochemistry

authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

更新日期：2001-12-18 00:00:00

abstract：:Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC 2.6.1.30) with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...

journal_title：Biochemistry

authors： Gilmer PJ,McIntire WS,Kirsch JF

更新日期：1977-11-29 00:00:00

abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...

journal_title：Biochemistry

authors： Joseph RE,Min L,Xu R,Musselman ED,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:Alpha-sarcin is an exquisitely specific ribonuclease that binds and cleaves a single phosphodiester bond in the large rRNA of the eukaryotic ribosome, inactivating it. To better understand this remarkable activity, the contributions of the active site residues (His 50, Glu 96, and His 137) to the conformational stabil...

journal_title：Biochemistry

authors： García-Mayoral MF,del Pozo AM,Campos-Olivas R,Gavilanes JG,Santoro J,Rico M,Laurents DV,Bruix M

更新日期：2006-11-21 00:00:00

abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...

journal_title：Biochemistry

authors： Tsai CH,Shen TJ,Ho NT,Ho C

更新日期：1999-07-06 00:00:00