NMR study of isoleucine transfer RNA from Thermus thermophilus.

abstract：:Stability increments of terminal unpaired nucleotides (dangling ends) and terminal base pairs on the core helixes AUGCAU and UGCGCA are reported. Enthalpy, entropy, and free energy changes of helix formation were measured spectrophotometrically for 18 oligoribonucleotides containing the core sequences. The results ind...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Turner DH

更新日期：1987-07-14 00:00:00

abstract：:The septins are a conserved family of guanosine-5'-triphosphate (GTP)-binding proteins. In mammals they are involved in a variety of cellular processes, such as cytokinesis, exocytosis, and vesicle trafficking. Specifically, SEPT4 has also been shown to be expressed in both human colorectal cancer and malignant melano...

journal_title：Biochemistry

authors： Garcia W,de Araújo AP,Neto Mde O,Ballestero MR,Polikarpov I,Tanaka M,Tanaka T,Garratt RC

更新日期：2006-11-21 00:00:00

abstract：:CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...

journal_title：Biochemistry

authors： Zolli M,Kobric DJ,Brown ED

更新日期：2001-04-24 00:00:00

abstract：:Previous work from this laboratory has established that the NADPH-menadione oxidoreductase reaction catalyzed by methylenetetrahydrofolate reductase from pig liver proceeds by Ping Pong Bi Bi kinetics and that the reductive half-reaction is rate limiting in steady-state turnover. We have now shown that methylenetetrah...

journal_title：Biochemistry

authors： Vanoni MA,Matthews RG

更新日期：1984-10-23 00:00:00

abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...

journal_title：Biochemistry

authors： Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

更新日期：2007-01-09 00:00:00

abstract：:The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...

journal_title：Biochemistry

authors： Musci G,Berliner LJ

更新日期：1986-08-26 00:00:00

abstract：:The regiospecificity and stereospecificity of proton transfer in the yeast inorganic pyrophosphatase (PPase) catalyzed hydrolysis of P1,P2-bidentate Mg(H2O)4(PPi)2- were probed with exchange-inert metal complexes of imidodiphosphate (PNP) and thiopyrophosphate (PPS). PPase was unable to catalyze the hydrolysis of Mg(H...

journal_title：Biochemistry

authors： Lin I,Knight WB,Hsueh A,Dunaway-Mariano D

更新日期：1986-08-12 00:00:00

abstract：:Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...

journal_title：Biochemistry

authors： Dai G,Zhang Y,Tamogami J,Demura M,Kamo N,Kandori H,Iwasa T

更新日期：2011-08-23 00:00:00

abstract：:The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a f...

journal_title：Biochemistry

authors： Nagata K,Kudo N,Abe K,Arai S,Tanokura M

更新日期：2000-12-05 00:00:00

abstract：:In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond (

journal_title：Biochemistry

authors： Shi X,Abbyad P,Shu X,Kallio K,Kanchanawong P,Childs W,Remington SJ,Boxer SG

更新日期：2007-10-30 00:00:00

abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...

journal_title：Biochemistry

authors： Pompliano DL,Schaber MD,Mosser SD,Omer CA,Shafer JA,Gibbs JB

更新日期：1993-08-17 00:00:00

abstract：:FTIR-difference spectroscopy in combination with site-directed mutagenesis has been used to investigate the role of water during the photocycle of bacteriorhodopsin. At least one water molecule is detected which undergoes an increase in H-bonding during the primary bR-->K phototransition. Bands due to water appear in ...

journal_title：Biochemistry

authors： Fischer WB,Sonar S,Marti T,Khorana HG,Rothschild KJ

更新日期：1994-11-01 00:00:00

abstract：:S-Adenosylmethionine decarboxylase is a pyruvate-dependent enzyme. The enzyme forms a Schiff base with substrate, S-adenosylmethionine, through the pyruvoyl moiety. This facilitates the release of CO2 from the substrate, which must then be protonated on the alpha carbon in order to permit hydrolysis of the Schiff base...

journal_title：Biochemistry

authors： Xiong H,Stanley BA,Pegg AE

更新日期：1999-02-23 00:00:00

abstract：:The present study focuses on ligand-protein interactions in a rhodopsin analogue generated from bovine opsin and the 10-methyl homologue of 11-cis-retinal. The analogue pigment displays a reduced alpha-band at 506 +/- 2 and a stronger beta-band at 325 nm. Remarkably, the rotational strength of these bands observed in ...

journal_title：Biochemistry

authors： DeLange F,Bovee-Geurts PH,VanOostrum J,Portier MD,Verdegem PJ,Lugtenburg J,DeGrip WJ

更新日期：1998-02-03 00:00:00

abstract：:The diversity, abundance, and DNA sequence representation of poly(adenylic acid) containing RNA derived from cells of Achlya ambisexualis cultured in defined and undefined media have been determined. The kinetics of hybridization of polyadenylated RNA with complementary DNA were the same for both culture conditions an...

journal_title：Biochemistry

authors： Rozek CE,Orr WC,Timberlake WE

更新日期：1978-02-21 00:00:00

abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...

journal_title：Biochemistry

authors： Dreveny I,Andryushkova AS,Glieder A,Gruber K,Kratky C

更新日期：2009-04-21 00:00:00

abstract：:The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...

journal_title：Biochemistry

authors： Wang Y,Zhang H,Ligon LA,McGown LB

更新日期：2009-09-01 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:The human cytosolic sulfotransferases (SULTs) regulate hundreds, perhaps thousands, of small molecule metabolites and xenobiotics via transfer of a sulfuryl moiety (-SO3) from PAPS (3'-phosphoadenosine 5'-phosphosulfate) to the hydroxyls and primary amines of the recipients. In liver, where it is abundant, SULT1A1 eng...

journal_title：Biochemistry

authors： Cook I,Wang T,Leyh TS

更新日期：2015-10-06 00:00:00

abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...

journal_title：Biochemistry

authors： Bradley M,Bücheler US,Walsh CT

更新日期：1991-06-25 00:00:00

abstract：:The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...

journal_title：Biochemistry

authors： Tweedy NB,Nair SK,Paterno SA,Fierke CA,Christianson DW

更新日期：1993-10-19 00:00:00

abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...

journal_title：Biochemistry

authors： Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice EC

更新日期：1988-07-12 00:00:00

abstract：:The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...

journal_title：Biochemistry

authors： Stennicke HR,Mortensen UH,Breddam K

更新日期：1996-06-04 00:00:00

abstract：:The generality of the concept of a rate-limiting step in enzymic reactions recently has been questioned [Northrop, D. B. (1981) Biochemistry 20, 4056-4061] because, in simulated isotopic experiments, alterations of the step identified as rate limiting by current definitions do not consistently affect Vmax in the expec...

journal_title：Biochemistry

authors： Ray WJ Jr

更新日期：1983-09-27 00:00:00

abstract：:The third intracellular loop (IL3) of G protein-coupled receptors (GPCRs) is an important contact domain between GPCRs and their G proteins. Previously, the IL3 of Ste2p, a Saccharomyces cerevisiae GPCR, was suggested to undergo a conformational change upon activation as detected by differential protease susceptibilit...

journal_title：Biochemistry

authors： Umanah GK,Huang LY,Maccarone JM,Naider F,Becker JM

更新日期：2011-08-16 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...

journal_title：Biochemistry

authors： Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

更新日期：2001-12-04 00:00:00

abstract：:Thyroxine and analogues inhibit rat liver aminoacyl-tRNA synthetase activity for phenylalanine and tyrosine. A high yield purification of the major cytoplasmic form of phenylalanyl-tRNA synthetase (C1) and its characterization is reported. Polyribosome-bound and other sedimentable forms are found to be indistinguishab...

journal_title：Biochemistry

authors： Nielsen JB,Haschemeyer AE

更新日期：1976-01-27 00:00:00

abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...

journal_title：Biochemistry

authors： Norman D,Live D,Sastry M,Lipman R,Hingerty BE,Tomasz M,Broyde S,Patel DJ

更新日期：1990-03-20 00:00:00

abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...

journal_title：Biochemistry

authors： Rutherford TJ,Neville DC,Homans SW

更新日期：1995-10-31 00:00:00