Abstract：
:The diversity, abundance, and DNA sequence representation of poly(adenylic acid) containing RNA derived from cells of Achlya ambisexualis cultured in defined and undefined media have been determined. The kinetics of hybridization of polyadenylated RNA with complementary DNA were the same for both culture conditions and revealed the presence of three frequency classes containing 29, 220, and 3000 different sequences of an average length of 1150 nucleotides. Complexity estimates derived from experiments in which polyadenylated RNA was hybridized to unique sequence DNA were in good agreement with these results. The kinetics of hybridization of complementary DNA with an excess of nuclear DNA indicate that approximately 10% of the RNA is transcribed from reiterated DNA sequences while the remainder is transcribed from single copy sequences.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rozek CE,Orr WC,Timberlake WEdoi
10.1021/bi00597a025subject
Has Abstractpub_date
1978-02-21 00:00:00pages
716-22issue
4eissn
0006-2960issn
1520-4995journal_volume
17pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Monte Carlo protein simulations with continuum solvation were used to explore the conformational mobility of NO within the active site of metmyoglobin. To the best of our knowledge this is the first application of a continuum solvation model for exploring protein binding sites. The usefulness of the Monte Carlo confor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981611v
更新日期：1999-05-18 00:00:00
abstract：:MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981066y
更新日期：1998-09-08 00:00:00
abstract：:By superimposing data obtained by photo-cross-linking RGD-containing ligands to the human alpha(V)beta(3) integrin onto the crystal structure of the ectopic domain of this receptor (Xiong et al. (2001) Science 294, 339-345), we have identified the binding site for the RGD triad within this integrin. We synthesized thr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025690t
更新日期：2002-07-02 00:00:00
abstract：:Molecular dynamics simulations of barnase have been conducted both in water and in 8 M urea solution for 500 ps at 25 degrees C and for 2000 ps at 85 degrees C. The final structure of the aqueous simulation at room temperature matches closely the structure obtained by NMR and the experimentally observed protections fr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970096i
更新日期：1997-06-17 00:00:00
abstract：:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs du...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00650a029
更新日期：1976-03-09 00:00:00
abstract：:Argonaute (AGO) proteins play central roles in nucleic acid-guided interference that regulates gene expression and defend against foreign genetic elements in all life. Although much progress has been made with respect to the function of argonaute proteins in target recognition and cleavage, the detailed mechanism of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00213
更新日期：2018-04-17 00:00:00
abstract：:The bacterial second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls important biological processes such as biofilm formation, virulence response, and motility. This second messenger is sensed by macromolecular targets inside the cell, both protein and RNA, which ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301510v
更新日期：2013-01-15 00:00:00
abstract：:Fluid-phase interactions between hematologic cells and those of the vessel wall were studied in order to define a role for lipoxygenase products as cell signals in the control of vascular cholesterol metabolism. A functional parameter for hydroxy acids in this system has not been previously demonstrated. We report her...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00448a030
更新日期：1989-10-31 00:00:00
abstract：:Pyrrolidone carboxyl peptidases (PCPs) from hyperthermophiles have a structurally conserved and completely buried Glu192 in the hydrophobic core; in contrast, the corresponding residue in the mesophile protein is a hydrophobic residue, Ile. Does the buried ionizable residue contribute to stabilization or destabilizati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052610n
更新日期：2006-06-13 00:00:00
abstract：:The E2 protein of papillomavirus is the key regulator of viral transcription and replication. Dimerization, which takes place via its conserved C-terminal DNA-binding domain (DBD), is critical for these functions. The presence and conservation of two histidines (H290 and H320) at or near the dimer interface suggests t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0611255
更新日期：2007-02-06 00:00:00
abstract：:The mobility of vesicular stomatitis virus (VSV) G protein on the surface of infected BHK cells was studied by using the technique of fluorescence photobleaching recovery. The fraction of surface G protein that was mobile in that time scale of the measurement (minutes) was at least 75%, a relatively high value among c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00508a047
更新日期：1981-03-03 00:00:00
abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970524k
更新日期：1997-10-07 00:00:00
abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00306a014
更新日期：1984-05-22 00:00:00
abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00117a032
更新日期：1992-01-21 00:00:00
abstract：:Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971771r
更新日期：1998-02-17 00:00:00
abstract：:The FTIR spectra for alkyl isocyanides (CNRs) change from a single nu(CN) band centered at approximately 2175 cm(-1) to two peaks at approximately 2075 and approximately 2125 cm(-1) upon binding to sperm whale myoglobin (Mb). The low- and high-frequency peaks have been assigned to in and out conformations, respectivel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100172c
更新日期：2010-06-22 00:00:00
abstract：:Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is als...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.9b00164
更新日期：2019-12-31 00:00:00
abstract：:The heme propionate groups of both myoglobin (Mb) and cytochrome b5 form hydrogen bonds with nearby surface amino acids residues that are believed to stabilize the heme-protein complex. To evaluate the magnitude of this stabilization, the kinetics of heme dissociation from variants of horse heart Mb and cytochrome b5 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961385u
更新日期：1997-02-04 00:00:00
abstract：:We describe a method for isolating milligram quantities of assembly-competent tubulin from the budding yeast Saccharomyces cerevisiae. The tubulin is > 95% purified and free of contaminating enzyme activities. As a result, it has been possible to determine the yeast tubulin equilibrium-binding constant for Mg-GTP and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00085a013
更新日期：1993-08-31 00:00:00
abstract：:The ionic and nucleotide requirements for the in vitro polymerization of microtubules from purified brain tubulin have been characterized by viscometry. Protein was purified by successive cycles of a temperature dependent assembly-diassembly scheme. Maximal polymerization occurred at a concentration of 0.1 M Pipes (pi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00684a032
更新日期：1975-07-01 00:00:00
abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200104h
更新日期：2011-05-31 00:00:00
abstract：:The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In this study, a method for expanding the genetic code of this cyanobacterium has been established, thereby allowing the incorporation of unn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00131
更新日期：2017-04-25 00:00:00
abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9521351
更新日期：1996-02-27 00:00:00
abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00937
更新日期：2020-02-04 00:00:00
abstract：:The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011001m
更新日期：2001-09-25 00:00:00
abstract：:Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701651k
更新日期：2007-11-13 00:00:00
abstract：:Mutations in the gene encoding human ATP:cobalamin adenosyltransferase (hATR) can result in the metabolic disorder known as methylmalonic aciduria (MMA). This enzyme catalyzes the final step in the conversion of cyanocobalamin (vitamin B12) to the essential human cofactor adenosylcobalamin. Here we present the 2.5 A c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061396f
更新日期：2006-12-26 00:00:00
abstract：:An endogenous calcium-activated sulfhydryl protease in chick oviduct cytosol has been utilized to study the structure of the chick oviduct progesterone receptor subunits, progestophilins A (79 000 g/mol) and B (117 000 g/mol). The protease is not a normal component of the native progesterone receptor aggregate (6 and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00543a015
更新日期：1980-01-22 00:00:00
abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102070w
更新日期：2011-05-10 00:00:00
abstract：:The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00127a036
更新日期：1992-03-31 00:00:00
