Methionine sulfoxide cytochrome c.

Abstract:

:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs during the photooxidation reaction. The absorbance spectrum of methionine sulfoxide ferricytochrome c at neutrality is similar to that of the unmodified protein except for an increase in the extinction coefficient of the Soret absorbance band and for the complete loss of the ligand sensitive 695 nm absorbance band in the spectrum of the derivative. The protein remains in the low spin configuration which implies the retention of two strong field ligands. Spin state sensitive spectral titrations and model studies of heme peptides indicate that the sixth ligand is definitely not provided by a lysine residue but may be methionine-80 sulfoxide coordinated via its sulfur atom. Circular dichroism spectra indicate that the heme crevice of methionine sulfoxide ferri- and ferrocytochrome c is weakened relative to native cytochrome c. The redox potential of methionine sulfoxide cytochrome c is 184 mV which is markedly diminished from the 260 mV redox potential of native cytochrome c. The modified protein is equivalent to native cytochrome c as a substrate for cytochrome oxidase and is not autoxidizable at neutral pH but is virtually inactive with succinate-cytochrome c reductase. These results indicate that the major role of the methionine-80 in cytochrome c is to preserve a closed hydrophobic heme crevice which is essential for the maintainance of the necessary redox potential.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ivanetich KM,Bradshaw JJ,Kaminsky LS

doi

10.1021/bi00650a029

subject

Has Abstract

pub_date

1976-03-09 00:00:00

pages

1144-53

issue

5

eissn

0006-2960

issn

1520-4995

journal_volume

15

pub_type

杂志文章
  • Comparison of lipid/gramicidin dispersions and cocrystals by Raman scattering.

    abstract::Gramicidin crystals, dimyristoylphosphatidylcholine (DMPC)/gramicidin dispersions, and DMPC/gramicidin cocrystals were examined by Raman scattering to determine lipid/gramicidin stoichiometries and lipid organization. Calibrations of the choline (716-cm-1) and tryptophan (756-cm-1) peaks indicate that the cocrystals c...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00376a030

    authors: Short KW,Wallace BA,Myers RA,Fodor SP,Dunker AK

    更新日期:1987-01-27 00:00:00

  • Control of the redox potential of cytochrome c and microscopic dielectric effects in proteins.

    abstract::X-ray structural information provides the opportunity to explore quantitatively the relation between the microenvironments of heme proteins and their redox potentials. This can be done by considering the protein as a "solvent" for its redox center and calculating the difference between the electrostatic energy of the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00355a035

    authors: Churg AK,Warshel A

    更新日期:1986-04-08 00:00:00

  • Reconstruction of quaternary structures of class II tRNA synthetases by rational mutagenensis of a conserved domain.

    abstract::Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971788+

    authors: Ribas de Pouplana L,Schimmel P

    更新日期:1997-12-09 00:00:00

  • Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons.

    abstract::In Sphingomonas CHY-1, a single ring-hydroxylating dioxygenase is responsible for the initial attack of a range of polycyclic aromatic hydrocarbons (PAHs) composed of up to five rings. The components of this enzyme were separately purified and characterized. The oxygenase component (ht-PhnI) was shown to contain one R...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0611311

    authors: Jouanneau Y,Meyer C,Jakoncic J,Stojanoff V,Gaillard J

    更新日期:2006-10-10 00:00:00

  • The active site structure of Thalassiosira weissflogii carbonic anhydrase 1.

    abstract::X-ray absorption spectroscopy at the Zn K-edge indicates that the active site of the marine diatom Thalassiosira weissflogii carbonic anhydrase is strikingly similar to that of mammalian alpha-carbonic anhydrase enzymes. The zinc has three histidine ligands and a single water at 1.98 A. This is quite different from th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001416s

    authors: Cox EH,McLendon GL,Morel FM,Lane TW,Prince RC,Pickering IJ,George GN

    更新日期:2000-10-10 00:00:00

  • Conservation of electrostatic properties within enzyme families and superfamilies.

    abstract::Electrostatic interactions play a key role in enzyme catalytic function. At long range, electrostatics steer the incoming ligand/substrate to the active site, and at short distances, electrostatics provide the specific local interactions for catalysis. In cases in which electrostatics determine enzyme function, orthol...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026918f

    authors: Livesay DR,Jambeck P,Rojnuckarin A,Subramaniam S

    更新日期:2003-04-01 00:00:00

  • Electron-transfer reactions in manganese-depleted photosystem II.

    abstract::We have used flash-detection optical and electron paramagnetic resonance spectroscopy to measure the kinetics and yield per flash of the photooxidation of cytochrome b559 and the yield per flash of the photooxidation of the tyrosine residue YD in Mn-depleted photosystem II (PSII) membranes at room temperature. The ini...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00490a014

    authors: Buser CA,Thompson LK,Diner BA,Brudvig GW

    更新日期:1990-09-25 00:00:00

  • Calorimetric studies of adenosine 5'-triphosphate hydrolysis by heavy meromyosin.

    abstract::The heat production for the reaction steps of ATP hydrolysis by heavy meromyosin has been reexamined by using a reaction-type calorimeter. So far, the values reported for the heat production have varied substantially among research groups. The present results obtained in 0.3 M KCl, 10 mM MgCl2, and 20 mM Tris-HCl at p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00518a037

    authors: Yamada T,Shimizu H,Suga H

    更新日期:1981-07-21 00:00:00

  • Pulse radiolysis studies on cytochrome cd1 nitrite reductase from Thiosphaera pantotropha: evidence for a fast intramolecular electron transfer from c-heme to d1-heme.

    abstract::Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971045o

    authors: Kobayashi K,Koppenhöfer A,Ferguson SJ,Tagawa S

    更新日期:1997-11-04 00:00:00

  • The active-site histidine-10 of enterococcal NADH peroxidase is not essential for catalytic activity.

    abstract::In order to test the proposal [Stehle, T., Claiborne, A., & Schulz, G. E. (1993) Eur. J. Biochem. 211, 221-226] that the active-site His10 of NADH peroxidase functions as an essential acid-base catalyst, we have analyzed mutants in which this residue has been replaced by Gln or Ala. The k(cat) values for both H10Q and...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952347y

    authors: Crane EJ 3rd,Parsonage D,Claiborne A

    更新日期:1996-02-20 00:00:00

  • The SH2 domains of inositol polyphosphate 5-phosphatases SHIP1 and SHIP2 have similar ligand specificity but different binding kinetics.

    abstract::SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9012462

    authors: Zhang Y,Wavreille AS,Kunys AR,Pei D

    更新日期:2009-11-24 00:00:00

  • Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions.

    abstract::The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011858j

    authors: Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

    更新日期:2001-12-25 00:00:00

  • Purified native subunits of bacterial luciferase are active in the bioluminescence reaction but fail to assemble into the alpha beta structure.

    abstract::We have expressed the alpha and beta subunits of bacterial luciferase, encoded by luxA and luxB, from separate plasmids in Escherichia coli and developed an efficient purification scheme that yields many milligrams of protein of greater than 90% homogeneity. Earlier experiments showed that subunits synthesized separat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00070a010

    authors: Sinclair JF,Waddle JJ,Waddill EF,Baldwin TO

    更新日期:1993-05-18 00:00:00

  • Purification and characterization of a transmembrane domain-deleted form of lecithin retinol acyltransferase.

    abstract::Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0342416

    authors: Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

    更新日期:2003-05-27 00:00:00

  • An additional H-bond in the alpha 1 beta 2 interface as the structural basis for the low oxygen affinity and high cooperativity of a novel recombinant hemoglobin (beta L105W).

    abstract::Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001115i

    authors: Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

    更新日期:2000-11-14 00:00:00

  • Kinetics of ganglioside transfer between liposomal and synaptosomal membranes.

    abstract::The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00375a032

    authors: Masserini M,Freire E

    更新日期:1987-01-13 00:00:00

  • Stabilization of the red semiquinone form of pig kidney general acyl-CoA dehydrogenase by acyl coenzyme A derivatives.

    abstract::Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00520a024

    authors: Mizzer JP,Thorpe C

    更新日期:1981-08-18 00:00:00

  • Investigations of photolysis and rebinding kinetics in myoglobin using proximal ligand replacements.

    abstract::We use laser flash photolysis and time-resolved Raman spectroscopy of CO-bound H93G myoglobin (Mb) mutants to study the influence of the proximal ligand on the CO rebinding kinetics. In H93G mutants, where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands (e.g., imidazole, 4-b...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049077g

    authors: Cao W,Ye X,Sjodin T,Christian JF,Demidov AA,Berezhna S,Wang W,Barrick D,Sage JT,Champion PM

    更新日期:2004-08-31 00:00:00

  • Phosphoryl transfer between phosphorylated histidine-containing protein and histidine-containing protein is not autocatalytic.

    abstract::Histidine-containing protein, HPr, is a phosphocarrier protein that is part of the bacterial phosphoenolpyruvate:sugar phosphotransferase system. HPr is phosphorylated by enzyme I, and P-HPr transfers the phosphoryl group to the IIA domain of a number of sugar-specific enzyme II complexes. Autocatalytic phosphoryl tra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00073a025

    authors: Anderson JW,Waygood EB

    更新日期:1993-06-08 00:00:00

  • Escherichia coli single-stranded DNA binding protein is mobile on DNA: 1H NMR study of its interaction with oligo- and polynucleotides.

    abstract::The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00320a036

    authors: Römer R,Schomburg U,Krauss G,Maass G

    更新日期:1984-12-04 00:00:00

  • Laser temperature jump study of solvent effects of poly(adenylic acid) stacking.

    abstract::The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00549a025

    authors: Dewey TG,Turner DH

    更新日期:1980-04-15 00:00:00

  • Importance of the region around glycine-338 for the activity of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system.

    abstract::The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952052k

    authors: Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

    更新日期:1996-01-09 00:00:00

  • Phospholipids occupy the internal lumen of the c ring of the ATP synthase of Escherichia coli.

    abstract::The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi052304+

    authors: Oberfeld B,Brunner J,Dimroth P

    更新日期:2006-02-14 00:00:00

  • Heteronuclear 3D NMR studies of water bound to an FK506 binding protein/immunosuppressant complex.

    abstract::From a series of 15N-resolved 3D ROESY-HMQC and 13C-resolved 3D NOESY-HMQC spectra of the FK506 binding protein (FKBP)/ascomycin complex in H2O, the locations of three tightly bound water molecules were identified. These waters are all buried within the interior of the complex and form an integral part of its structur...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00061a004

    authors: Xu RX,Meadows RP,Fesik SW

    更新日期:1993-03-16 00:00:00

  • Heme synthase (ferrochelatase) catalyzes the removal of iron from heme and demetalation of metalloporphyrins.

    abstract::The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701460x

    authors: Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

    更新日期:2007-12-25 00:00:00

  • Decrease in ADP-ribosylation of HeLa non-histone proteins from interphase to metaphase.

    abstract::Variations for non-histones in the ADP-ribosylating activities of interphase and metaphase cells were investigated. 32P-Labeled nicotinamide adenine dinucleotide ([32P]NAD), the specific precursor for the modification, was used to radioactively label proteins. Permeabilized interphase and mitotic cells, as well as iso...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00323a017

    authors: Adolph KW,Song MK

    更新日期:1985-01-15 00:00:00

  • Comparative mutagenesis of O6-methylguanine and O4-methylthymine in Escherichia coli.

    abstract::The qualitative and quantitative features of mutagenesis by two DNA adducts of carcinogenic alkylating agents, O6-methylguanine (m6G) and O4-methylthymine (m4T), were examined in vivo. The deoxyhexanucleotides 5'-GCTAGC-3' and 5'-GCTAGC-3' were synthesized, where the underlined bases are the positions of m4T or m6G, r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00242a031

    authors: Dosanjh MK,Singer B,Essigmann JM

    更新日期:1991-07-16 00:00:00

  • Isothermal titration calorimetric studies on the binding of deoxytrimannoside derivatives with artocarpin: implications for a deep-seated combining site in lectins.

    abstract::The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000744p

    authors: Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

    更新日期:2000-09-05 00:00:00

  • Design and expression of cysteine-bearing hydrophobic polypeptides and their self-assembling properties with bacteriochlorophyll a derivatives as a mimic of bacterial photosynthetic antenna complexes. Effect of steric confinement and orientation of the po

    abstract::A series of cysteine-bearing hydrophobic polypeptides analogous to a light-harvesting one betapolypeptide (LH1beta) from the LH1 complex from the purple photosynthetic bacterium, Rhodobacter sphaeroides, was synthesized using an Escherichia coli expression system. The cysteine was placed in the C- or N-terminal region...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0478493

    authors: Dewa T,Yamada T,Ogawa M,Sugimoto M,Mizuno T,Yoshida K,Nakao Y,Kondo M,Iida K,Yamashita K,Tanaka T,Nango M

    更新日期:2005-04-05 00:00:00

  • Probing the bimolecular interactions of parathyroid hormone with the human parathyroid hormone/parathyroid hormone-related protein receptor. 1. Design, synthesis and characterization of photoreactive benzophenone-containing analogs of parathyroid hormone.

    abstract::Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00033a029

    authors: Nakamoto C,Behar V,Chin KR,Adams AE,Suva LJ,Rosenblatt M,Chorev M

    更新日期:1995-08-22 00:00:00