Identification of c-di-GMP derivatives resistant to an EAL domain phosphodiesterase.

abstract：:Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...

journal_title：Biochemistry

authors： Blanchet J,Lin SX,Zhorov BS

更新日期：2005-05-17 00:00:00

abstract：:Simple two-dimensional thin-layer chromatography was found to be useful for the separation of sugar methylated dinucleotides in RNA. Of the 16 possible sequences of the type Nm-Np, 15 were separated and all the sequences were determined. In a mouse hepatoma, MH 134, the levels of the sugar methylation in the 18S and 2...

journal_title：Biochemistry

authors： Hashimoto S,Sakai M,Muramatsu M

更新日期：1975-05-06 00:00:00

abstract：:The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...

journal_title：Biochemistry

authors： Cai S,Stevens SY,Budor AP,Zuiderweg ER

更新日期：2003-09-30 00:00:00

abstract：:The heme environmental structures of lactoperoxidase (LP) have been studied by the use of hyperfine-shifted proton NMR and optical absorption spectra. The NMR spectra of the enzyme in native and cyanide forms in H2O indicated that the fifth ligand of the heme iron is the histidyl imidazole with an anionic character an...

journal_title：Biochemistry

authors： Shiro Y,Morishima I

更新日期：1986-10-07 00:00:00

abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...

journal_title：Biochemistry

authors： Sancho J,Serrano L,Fersht AR

更新日期：1992-03-03 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...

journal_title：Biochemistry

authors： Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

更新日期：2013-11-12 00:00:00

abstract：:The heterotrimeric G protein subunit, alpha(s), can move reversibly from plasma membranes to cytoplasm in response to activation by GPCRs or activating mutations. We examined the importance of the unique N-terminus of alpha(s) in this translocation in cultured cells. alpha(s) contains a single site for palmitoylation ...

journal_title：Biochemistry

authors： Thiyagarajan MM,Bigras E,Van Tol HH,Hébert TE,Evanko DS,Wedegaertner PB

更新日期：2002-07-30 00:00:00

abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...

journal_title：Biochemistry

authors： Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

更新日期：2005-10-25 00:00:00

abstract：:An important but poorly characterized contribution to the thermodynamics of protein-DNA interactions is the loss of entropy that occurs from restricting the conformational freedom of amino acid side chains. The effect of restricting the flexibility of several side chains at a protein-DNA interface may be comparable in...

journal_title：Biochemistry

authors： Baird-Titus JM,Thapa M,Doerdelmann T,Combs KA,Rance M

更新日期：2018-05-15 00:00:00

abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...

journal_title：Biochemistry

authors： Chau MH,Nelson JW

更新日期：1992-05-12 00:00:00

abstract：:It is now appreciated that the relationships of proteins, particularly enzymes, within a protein superfamily can be understood not only in terms of their sequence similarities and three-dimensional structures but also by chemical threads that relate their functional attributes. The mechanistic ties among superfamily m...

journal_title：Biochemistry

authors： Armstrong RN

更新日期：2000-11-14 00:00:00

abstract：:Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...

journal_title：Biochemistry

authors： Firestine SM,Davisson VJ

更新日期：1994-10-04 00:00:00

abstract：:To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...

journal_title：Biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2000-12-19 00:00:00

abstract：:Incubation of bovine plasma amine oxidase (BPAO) with benzylamine and various p-substituted analogues results in a time-dependent inactivation that is attributable to buildup of the H(2)O(2)-turnover product on the basis of protection afforded by coincubation with catalase. The mechanism of inactivation is distinct fr...

journal_title：Biochemistry

authors： Lee Y,Shepard E,Smith J,Dooley DM,Sayre LM

更新日期：2001-01-23 00:00:00

abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...

journal_title：Biochemistry

authors： Kikkawa S,Tominaga K,Nakagawa M,Iwasa T,Tsuda M

更新日期：1996-12-10 00:00:00

abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...

journal_title：Biochemistry

authors： Lee R,Kaushik N,Modak MJ,Vinayak R,Pandey VN

更新日期：1998-01-20 00:00:00

abstract：:The Iowa point mutation in apolipoprotein A-I (G26R) leads to a systemic amyloidosis condition, and the Milano mutation (R173C) is associated with hypoalphalipoproteinemia, a reduced plasma level of high-density lipoprotein. To probe the structural effects that lead to these outcomes, we used amide hydrogen-deuterium ...

journal_title：Biochemistry

authors： Chetty PS,Ohshiro M,Saito H,Dhanasekaran P,Lund-Katz S,Mayne L,Englander W,Phillips MC

更新日期：2012-11-06 00:00:00

abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

journal_title：Biochemistry

authors： Wagner PD,Stone DB

更新日期：1983-03-15 00:00:00

abstract：:An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...

journal_title：Biochemistry

authors： Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

更新日期：2001-11-13 00:00:00

abstract：:The electrons extracted from the CaMn(4) cluster during water oxidation in photosystem II are transferred to P(680)(+) via the redox-active tyrosine D1-Tyr161 (Y(Z)). Upon Y(Z) oxidation a proton moves in a hydrogen bond toward D1-His190 (His(Z)). The deprotonation and reprotonation mechanism of Y(Z)-OH/Y(Z)-O is of k...

journal_title：Biochemistry

authors： Sjöholm J,Havelius KG,Mamedov F,Styring S

更新日期：2010-11-16 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Beta-lactamases are the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and cephalosporins. Transition-state analogues that bind to the enzymes with nanomolar affinities have been introduced in an effort to reverse the resistance conferred by these enzymes. To understand the or...

journal_title：Biochemistry

authors： Roth TA,Minasov G,Morandi S,Prati F,Shoichet BK

更新日期：2003-12-16 00:00:00

abstract：:The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...

journal_title：Biochemistry

authors： Luly P,Shinitzky M

更新日期：1979-02-06 00:00:00

abstract：:Phe-tRNAPhe species modified on the 3'-terminal ribose residue were investigated for their ability to participate in individual steps of the elongation cycle using eukaryotic ribosomes from reticulocytes. None of the Phe-tRNAs used, namely Phe-tRNAPhe-C-C-3'dA, Phe-tRNAPhe-C-C-3'-NH2A, and Phe-tRNAPhe-C-C-Aoxi-red, ca...

journal_title：Biochemistry

authors： Baksht E,de Groot N,Sprinzl M,Cramer F

更新日期：1976-08-10 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...

journal_title：Biochemistry

authors： Zaheer A,Lim R

更新日期：1996-05-21 00:00:00

abstract：:The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...

journal_title：Biochemistry

authors： Hurshman AR,White JT,Powers ET,Kelly JW

更新日期：2004-06-15 00:00:00

abstract：:The assignment of disulfide bonds in human J chain and its covalent pairing with immunoglobulin M was determined under conditions which minimize disulfide bond interchange. We show that in J chain the three intradisulfide bridges are formed between Cys 12 and 100, Cys 71 and 91, and Cys 108 and 133. Previous reports [...

journal_title：Biochemistry

authors： Frutiger S,Hughes GJ,Paquet N,Lüthy R,Jaton JC

更新日期：1992-12-22 00:00:00

abstract：:Allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. Several observations suggested that the usual allosteric mechanism might not apply to the activation of ...

journal_title：Biochemistry

authors： Riera TV,Zheng L,Josephine HR,Min D,Yang W,Hedstrom L

更新日期：2011-10-04 00:00:00