Effects of pH on the S(3) state of the oxygen evolving complex in photosystem II probed by EPR split signal induction.

abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...

journal_title：Biochemistry

authors： Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini A

更新日期：1994-05-31 00:00:00

abstract：:The diffusion of protons at the immediate vicinity of (less than 10 A from) a phospholipid membrane is studied by the application of the laser-induced proton pulse. A light-sensitive proton emitter (8-hydroxypyrene-1,3,6-trisulfonate) was trapped exclusively in the hydration layers of multilamellar vesicles made of eg...

journal_title：Biochemistry

authors： Gutman M,Nachliel E,Moshiach S

更新日期：1989-04-04 00:00:00

abstract：:Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

更新日期：1993-11-09 00:00:00

abstract：:A series of secreted proteases are included among the virulence factors documented for Staphylococcus aureus. In light of increasing antibiotic resistance of this dangerous human pathogen, these proteases are considered as suitable targets for the development of novel therapeutic strategies. The recent discovery of st...

journal_title：Biochemistry

authors： Dubin G,Krajewski M,Popowicz G,Stec-Niemczyk J,Bochtler M,Potempa J,Dubin A,Holak TA

更新日期：2003-11-25 00:00:00

abstract：:We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...

journal_title：Biochemistry

authors： Christian EL,Yarus M

更新日期：1993-05-04 00:00:00

abstract：:Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be ...

journal_title：Biochemistry

authors： Kean JM,Murakami A,Blake KR,Cushman CD,Miller PS

更新日期：1988-12-27 00:00:00

abstract：:The bacterial photosynthetic reaction center is the evolutionary ancestor of the Photosystem II reaction center. These proteins share the same fold and perform the same biological function. Nevertheless, the details of their molecular reaction mechanism differ. It is of significant biological and biochemical interest ...

journal_title：Biochemistry

authors： Krammer EM,Sebban P,Ullmann GM

更新日期：2009-02-17 00:00:00

abstract：:The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...

journal_title：Biochemistry

authors： Wang Y,Zhang H,Ligon LA,McGown LB

更新日期：2009-09-01 00:00:00

abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...

journal_title：Biochemistry

authors： Fewtrell C,Kempner E,Poy G,Metzger H

更新日期：1981-11-10 00:00:00

abstract：:Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the lig...

journal_title：Biochemistry

authors： Saroff HA

更新日期：1991-10-22 00:00:00

abstract：:The immunoglobulin E-binding protein, epsilon BP (also known as CBP35, Mac-2, L-34, and L-29), is a beta-galactoside-binding protein of approximately 30 kDa and a member of the animal lectin family termed S-type or S-Lac. Multiple biological activities have been attributed to this lectin such as mediation of IgE bindi...

journal_title：Biochemistry

authors： Feizi T,Solomon JC,Yuen CT,Jeng KC,Frigeri LG,Hsu DK,Liu FT

更新日期：1994-05-24 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:Many lipases are potent catalysts of stereoselective reactions and are therefore of interest for use in chemical synthesis. The crystal structures of lipases show a large variation in the shapes of their active site environments that may explain the large variation in substrate specificity of these enzymes. We have de...

journal_title：Biochemistry

authors： Uppenberg J,Ohrner N,Norin M,Hult K,Kleywegt GJ,Patkar S,Waagen V,Anthonsen T,Jones TA

更新日期：1995-12-26 00:00:00

abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

journal_title：Biochemistry

authors： Arndt JW,Chai Q,Christian T,Stevens RC

更新日期：2006-03-14 00:00:00

abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...

journal_title：Biochemistry

authors： Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

更新日期：2019-04-23 00:00:00

abstract：:Factor V(a) (FV(a)) is a cofactor for the serine protease factor X(a) that activates prothrombin to thrombin in the presence of Ca(2+) and a membrane surface. FV(a) is a heterodimer composed of one heavy chain (A1 and A2 domains) and one light chain (A3, C1, and C2 domains). We use fluorescence, circular dichroism, an...

journal_title：Biochemistry

authors： Srivastava A,Quinn-Allen MA,Kim SW,Kane WH,Lentz BR

更新日期：2001-07-27 00:00:00

abstract：:This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR...

journal_title：Biochemistry

authors： Thapar R,Nicholson EM,Rajagopal P,Waygood EB,Scholtz JM,Klevit RE

更新日期：1996-09-03 00:00:00

abstract：:Cdc42 and Rac are highly homologous members of the Rho family of small G proteins that interact with several downstream effector proteins thereby causing cytoskeletal rearrangements, cell proliferation, and differentiation. While some effectors, such as the tyrosine kinase, ACK, and the scaffold protein, WASP, are uni...

journal_title：Biochemistry

authors： Elliot-Smith AE,Mott HR,Lowe PN,Laue ED,Owen D

更新日期：2005-09-20 00:00:00

abstract：:We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I) molecules on the cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crysta...

journal_title：Biochemistry

authors： Sun X,Tokunaga R,Nagai Y,Miyahara R,Kishimura A,Kawakami S,Katayama Y,Mori T

更新日期：2020-12-15 00:00:00

abstract：:AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...

journal_title：Biochemistry

authors： Reynolds CM,Poole LB

更新日期：2001-04-03 00:00:00

abstract：:Soybean lipoxygenase 1 was studied using limited proteolysis and active-site labeling to begin the structural characterization of the enzyme in solution. The serine proteases trypsin and chymotrypsin cleaved the large monomeric protein (95 kDa) into two large polypeptides, a C-terminal fragment of about 30 kDa and an ...

journal_title：Biochemistry

authors： Ramachandran S,Carroll RT,Dunham WR,Funk MO Jr

更新日期：1992-08-25 00:00:00

abstract：:Spectroscopic and kinetic methods have been used to explore the roles of divalent metal ions in the enolase-catalyzed dehydration of 2-phosphoglycerate (2-PGA). Enolase requires 2 equiv of metal ion per active site for maximal activity. Previous crystallographic studies [Larsen, T. M., Wedekind, J. E., Rayment, I., an...

journal_title：Biochemistry

authors： Poyner RR,Cleland WW,Reed GH

更新日期：2001-07-10 00:00:00

abstract：:Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

journal_title：Biochemistry

authors： Korman TP,Tan YH,Wong J,Luo R,Tsai SC

更新日期：2008-02-19 00:00:00

abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...

journal_title：Biochemistry

authors： Davey MW,Sulkowski E,Carter WA

更新日期：1976-02-10 00:00:00

abstract：:Several naturally occurring dietary polyphenols with chemopreventive or anticancer properties are topoisomerase II poisons. To identify additional phytochemicals that enhance topoisomerase II-mediated DNA cleavage, a library of 341 Mediterranean plant extracts was screened for activity against human topoisomerase IIα....

journal_title：Biochemistry

authors： Vann KR,Sedgeman CA,Gopas J,Golan-Goldhirsh A,Osheroff N

更新日期：2015-07-28 00:00:00

abstract：:N-(1-Pyrenyl)iodoacetamide has been used to introduce fluorescent probes into Escherichia coli RNA polymerase. After an incubation time of 15 min, approximately 2 pyrene equiv was introduced per enzyme molecule. There was no further increase in modification after more extended periods of incubation. Neither calf thymu...

journal_title：Biochemistry

authors： Johnson RS,Bowers M,Eaton Q

更新日期：1991-01-08 00:00:00

abstract：:The production of proteins via recombinant DNA technology often requires the in vitro folding of inclusion bodies, which are protein aggregates. To create a more efficient redox buffer for the in vitro folding of disulfide containing proteins, aromatic thiols were investigated for their ability to increase the folding...

journal_title：Biochemistry

authors： Gough JD,Gargano JM,Donofrio AE,Lees WJ

更新日期：2003-10-14 00:00:00

abstract：:Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...

journal_title：Biochemistry

authors： Gopal B,Madan LL,Betz SF,Kossiakoff AA

更新日期：2005-01-11 00:00:00

abstract：:Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...

journal_title：Biochemistry

authors： Burt AJ,Hantho JD,Nielsen AE,Mancini RJ

更新日期：2018-04-17 00:00:00

abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...

journal_title：Biochemistry

authors： Breton J

更新日期：2007-04-17 00:00:00