Abstract:
:The septins are a conserved family of guanosine-5'-triphosphate (GTP)-binding proteins. In mammals they are involved in a variety of cellular processes, such as cytokinesis, exocytosis, and vesicle trafficking. Specifically, SEPT4 has also been shown to be expressed in both human colorectal cancer and malignant melanoma, as well as being involved in neurodegenerative disorders. However, many of the details of the modes of action of septins in general remain unclear, and little is known of their detailed molecular architecture. Here, we define explicitly and characterize the domains of human SEPT4. Regions corresponding to the N-terminal, GTPase, and C-terminal domains as well as the latter two together were successfully expressed in Escherichia coli in soluble form and purified by affinity and size-exclusion chromatographies. The purified domains were analyzed by circular dichroism spectroscopy, fluorescence spectroscopy, dynamic light scattering, and small-angle X-ray scattering, as well as with bioinformatics tools. Of the three major domains that comprise SEPT4, the N-terminal domain contains little regular secondary structure and may be intrinsically unstructured. The central GTPase domain is a mixed alpha/beta structure, probably based on an open beta sheet. As defined here, it is catalytically active and forms stable homodimers in vitro. The C-terminal domain also forms homodimers and can be divided into two regions, the second of which is alpha-helical and consistent with a coiled-coil structure. These studies should provide a useful basis for future biophysical studies of SEPT4, including the structural basis for their involvement in diseases such as cancer and neurodegenerative disorders.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Garcia W,de Araújo AP,Neto Mde O,Ballestero MR,Polikarpov I,Tanaka M,Tanaka T,Garratt RCdoi
10.1021/bi061549zsubject
Has Abstractpub_date
2006-11-21 00:00:00pages
13918-31issue
46eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00218a034
更新日期:1991-01-29 00:00:00
abstract::A critical point of interaction between F(1) and F(0) in the bacterial F(1)F(0)-ATP synthase is formed by the alpha and delta subunits. Previous work has shown that the N-terminal domain (residues 3-105) of the delta subunit forms a 6 alpha-helix bundle [Wilkens, S., Dunn, S. D., Chandler, J., Dahlquist, F. W., and Ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0510678
更新日期:2005-09-06 00:00:00
abstract::Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501342w
更新日期:2014-12-16 00:00:00
abstract::Leucine aminopeptidase (LAP) is a protease requiring two divalent metal cations per subunit for activity. Zn2+, Mg2+, and Co2+ metal-substituted forms of LAP have been prepared and investigated kinetically. Substitution of metal into the two binding sites independently resulted in the preparation of Zn2+Zn2+, Mg2+Zn2+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00285a010
更新日期:1983-08-02 00:00:00
abstract::Factors which influence the accessibility, or exposure, of the carbohydrate head group of the glycolipid galactosylceramide (GalCer) at the membrane surface have been examined in lipid model membranes using the technique of galactose oxidase-tritiated sodium borohydride labeling. Both the ceramide composition of GalCe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00072a016
更新日期:1993-06-01 00:00:00
abstract::Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962175k
更新日期:1996-12-17 00:00:00
abstract::The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001206f
更新日期:2000-10-24 00:00:00
abstract::The polysaccharide-rich cell walls (CWs) of plants perform essential functions such as maintaining tensile strength and allowing plant growth. Using two- and three-dimensional magic-angle-spinning (MAS) solid-state NMR and uniformly (13)C-labeled Arabidopsis thaliana, we have assigned the resonances of the major polys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101795q
更新日期:2011-02-15 00:00:00
abstract::The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9626158
更新日期:1997-04-01 00:00:00
abstract::The orientation of the myosin neck was monitored using electron paramagnetic resonance (EPR) spectroscopy. Gizzard regulatory light chain was labeled with a nitroxide spin probe and exchanged for the native subunit, located in the myosin neck, in rabbit psoas muscle fibers. The EPR spectra of rigor fibers indicated a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960505v
更新日期:1996-08-06 00:00:00
abstract::Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00250a022
更新日期:1994-11-22 00:00:00
abstract::Protocatechuate 3,4-dioxygenase (3,4-PCD) utilizes a ferric ion to catalyze the aromatic ring cleavage of 3,4-dihydroxybenzoate (PCA) by incorporation of both atoms of dioxygen to yield beta-carboxy-cis, cis-muconate. The crystal structures of the anaerobic 3,4-PCD.PCA complex, aerobic complexes with two heterocyclic ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970469f
更新日期:1997-08-19 00:00:00
abstract::The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00357a013
更新日期:1986-05-06 00:00:00
abstract::Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7018964
更新日期:2008-03-18 00:00:00
abstract::The intact photosystem I of maize containing its full antenna complement (PSI-200) has been purified and fractionated into the core and outer antenna (LHCI) components. It is demonstrated by absorption and fluorescence spectroscopy that at least 80% of the long wavelength absorbing antenna pigments (red forms) are loc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9813227
更新日期:1998-12-15 00:00:00
abstract::In Salmonella typhimurium, formation of the cobalt-carbon bond in the biosynthetic pathway for adenosylcobalamin is catalyzed by the product of the cobA gene which encodes a protein of 196 amino acid residues. This enzyme is an ATP:co(I)rrinoid adenosyltransferase which transfers an adenosyl moiety from MgATP to a bro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002145o
更新日期:2001-01-16 00:00:00
abstract::R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034643d
更新日期:2003-09-16 00:00:00
abstract::Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035924o
更新日期:2004-04-06 00:00:00
abstract::Many drugs are oxidized by membrane protein cytochrome P450 (CYP) enzymes during their metabolism process. CYPs are located mainly in endoplasmic reticulum (ER) membranes. Recent studies have suggested that CYP substrate drugs first bind the lipid bilayers of ER membranes and then the drugs reach the active site of CY...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00286
更新日期:2016-07-19 00:00:00
abstract::The dynamic equilibrium of a catalytic site between active and inactive conformations, the missing link between the structure and function of allosteric enzymes, was identified using protein engineering and NMR techniques. Kinetic analyses of the wild-type and three mutants of Thermus L-lactate dehydrogenase establish...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00138a017
更新日期:1992-06-16 00:00:00
abstract::Type 1 diabetes (T1D) is an autoimmune disease characterized by an insulin deficiency. Ever since the discovery of insulin almost 100 years ago, patients with T1D have relied on multiple daily insulin injections to survive an otherwise deadly disease. Despite decades of research and clinical trials, no treatment exist...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.9b00707
更新日期:2019-10-08 00:00:00
abstract::The RmFixL-RmFixJ oxygen signal transduction system ensures that a cascade of the Sinorhizobium meliloti nitrogen fixation genes is induced as the concentration of O2 drops below 50 microM in symbiotic nodules. Deoxy-RmFixL is a histidine protein kinase that catalyzes a phosphoryl transfer from ATP to the aspartate 54...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051661h
更新日期:2005-11-22 00:00:00
abstract::Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701812c
更新日期:2007-12-25 00:00:00
abstract::The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9517751
更新日期:1996-02-13 00:00:00
abstract::The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0006971
更新日期:2000-12-05 00:00:00
abstract::One of the putative proton-transfer pathways leading from solution toward the binuclear center in many cytochrome c oxidases is the D-pathway, so-called because it starts with a highly conserved aspartate [D(I-132)] residue. Another highly conserved amino acid residue in this pathway, glutamate(I-286), has been indica...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0012641
更新日期:2000-12-26 00:00:00
abstract::The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9910880
更新日期:1999-09-14 00:00:00
abstract::The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00671a009
更新日期:1976-12-28 00:00:00
abstract::The two dominant glucolipids in Acholeplasma laidlawii, viz., 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDG) and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1----2)-O-alpha-D-glucopyranosyl ]- sn-glycerol (DGlcDG), have markedly different phase behavior. MGlcDG has an ability to form nonlamellar phases, wher...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00234a012
更新日期:1991-05-21 00:00:00
abstract::The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00566a010
更新日期:1980-12-09 00:00:00