Resonance raman analysis of chromophore structure in the lumi-R photoproduct of phytochrome.

abstract：:Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...

journal_title：Biochemistry

authors： Nienaber VL,Breddam K,Birktoft JJ

更新日期：1993-11-02 00:00:00

abstract：:Cells cultured from human skin synthesize precursor forms of types I and III collagens. After denaturation and reduction, the polypeptide chains obtained from these molecules had molecular weights estimated to be 140,000 and 120, 000. The larger chains, the pro alpha chains, are believed to be derived from the origina...

journal_title：Biochemistry

authors： Lichtenstein JR,Byers PH,Smith BD,Martin GR

更新日期：1975-04-22 00:00:00

abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

journal_title：Biochemistry

authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

更新日期：1984-08-28 00:00:00

abstract：:The importance of the interdomain bridging interactions observed only in the R-state structure of Escherichia coli aspartate transcarbamylase between Glu-50 of the carbamoyl phosphate domain with both Arg-167 and Arg-234 of the aspartate domain has been investigated by using site-specific mutagenesis. Two mutant versi...

journal_title：Biochemistry

authors： Newton CJ,Kantrowitz ER

更新日期：1990-02-13 00:00:00

abstract：:Polyamides composed of N-methylpyrrole (Py) and N-methylimidazole (Im) subunits can bind in the minor groove of DNA at predetermined sequences with subnanomolar affinity and high specificity. Covalent linkage of polymer subunits using a gamma-aminobutyric acid linker has been shown to increase both the affinity and sp...

journal_title：Biochemistry

authors： Baliga R,Baird EE,Herman DM,Melander C,Dervan PB,Crothers DM

更新日期：2001-01-09 00:00:00

abstract：:Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...

journal_title：Biochemistry

authors： Witt JJ,Roskoski R Jr

更新日期：1980-01-08 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...

journal_title：Biochemistry

authors： Luck LA,Falke JJ

更新日期：1991-04-30 00:00:00

abstract：:FTIR-difference spectroscopy in combination with site-directed mutagenesis has been used to investigate the role of water during the photocycle of bacteriorhodopsin. At least one water molecule is detected which undergoes an increase in H-bonding during the primary bR-->K phototransition. Bands due to water appear in ...

journal_title：Biochemistry

authors： Fischer WB,Sonar S,Marti T,Khorana HG,Rothschild KJ

更新日期：1994-11-01 00:00:00

abstract：:Baeyer-Villiger monooxygenases (BVMOs), mostly flavoproteins, were shown to be powerful biocatalysts for synthetic organic chemistry applications and were also suggested to play key roles for the biosyntheses of various natural products. Here we present the three-dimensional structure of MtmOIV, a 56 kDa homodimeric F...

journal_title：Biochemistry

authors： Beam MP,Bosserman MA,Noinaj N,Wehenkel M,Rohr J

更新日期：2009-06-02 00:00:00

abstract：:The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...

journal_title：Biochemistry

authors： Qin J,Clore GM,Gronenborn AM

更新日期：1996-01-09 00:00:00

abstract：:The ATP-hydrolyzing portion of the proton pump from clathrin-coated vesicles (isolated from calf brain) was solubilized with three nondenaturing detergents (cholate, octyl glucoside, and Triton X-100). The hydrodynamic properties of the solubilized (Mg2+)-ATPase were then determined by sedimentation analysis in H2O an...

journal_title：Biochemistry

authors： Forgac M,Berne M

更新日期：1986-07-29 00:00:00

abstract：:The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...

journal_title：Biochemistry

authors： Sakamoto T,Yildez A,Selvin PR,Sellers JR

更新日期：2005-12-13 00:00:00

abstract：:There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...

journal_title：Biochemistry

authors： Khan IA,Ludueña RF

更新日期：1996-03-26 00:00:00

abstract：:A new procedure for the synthesis of the pyrophosphate bond has been employed in the preparation of nucleoside dipyrophosphates from nucleoside 3',5'-diphosphates. The method makes use of a powerful phosphorylating agent generated in a mixture of cyanoethyl phosphate, dicyclohexylcarbodiimide, and mesitylenesulfonyl c...

journal_title：Biochemistry

authors： Bennett GN,Gough GR,Gilham PT

更新日期：1976-10-19 00:00:00

abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

journal_title：Biochemistry

authors： Kaur H,Scaria V,Maiti S

更新日期：2010-11-09 00:00:00

abstract：:The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtai...

journal_title：Biochemistry

authors： Stumph WE,Wu JR,Bonner J

更新日期：1979-06-26 00:00:00

abstract：:The specific effect of the binding of IF2 and initiator fMET-tRNA(fMet) on Escherichia coli 16S rRNA has been probed by phosphate alkylation with ethylnitrosourea. The results show that IF2 does not significantly shield portions of 16S rRNA but induces both reductions and enhancements of reactivity scattered in the en...

journal_title：Biochemistry

authors： Wakao H,Romby P,Laalami S,Ebel JP,Ehresmann C,Ehresmann B

更新日期：1990-09-04 00:00:00

abstract：:S-Adenosylmethionine decarboxylase, a pyruvoyl-containing decarboxylase, is inactivated in a time-dependent process under turnover conditions. The inactivation is dependent on the presence of both substrate and Mg2+, which is also required for enzyme activity. The rate of inactivation is dependent on the concentration...

journal_title：Biochemistry

authors： Anton DL,Kutny R

更新日期：1987-10-06 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:The anaerobic global regulator FNR from Escherichia coli is a [4Fe-4S](2+) cluster-containing dimer that is inactivated by O(2) through disruption of the Fe-S cluster and conversion to the monomeric apoprotein. It was shown that apo-FNR is subject to ClpXP proteolysis, and two recognition sites, amino acids 5-11 and a...

journal_title：Biochemistry

authors： Pan Q,Shan Y,Yan A

更新日期：2012-06-26 00:00:00

abstract：:The binding site of the dopamine D2 receptor, like that of other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using the substituted-cysteine accessibility method, we previously mapped the residues in the third, fifth, and seve...

journal_title：Biochemistry

authors： Javitch JA,Ballesteros JA,Weinstein H,Chen J

更新日期：1998-01-27 00:00:00

abstract：:The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...

journal_title：Biochemistry

authors： Strange RW,Reinhammar B,Murphy LM,Hasnain SS

更新日期：1995-01-10 00:00:00

abstract：:Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...

journal_title：Biochemistry

authors： Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

更新日期：1986-10-21 00:00:00

abstract：:Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...

journal_title：Biochemistry

authors： Mieyal JJ,Starke DW,Gravina SA,Dothey C,Chung JS

更新日期：1991-06-25 00:00:00

abstract：:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA)...

journal_title：Biochemistry

authors： Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NT

更新日期：1986-11-04 00:00:00

abstract：:The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LA...

journal_title：Biochemistry

authors： Perez-Villar JJ,Whitney GS,Sitnick MT,Dunn RJ,Venkatesan S,O'Day K,Schieven GL,Lin TA,Kanner SB

更新日期：2002-08-27 00:00:00

abstract：:Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...

journal_title：Biochemistry

authors： Buchko GW,Weinfeld M

更新日期：1993-03-09 00:00:00

abstract：:Respiratory complex I couples the electron transfer exclusively from NADH to a quinone with the translocation of protons across the membrane. However, Escherichia coli adapts to imposed high cellular NADPH concentrations by selecting the mutations E183A(F) and E183G(F) that lead to a high catalytic efficiency of compl...

journal_title：Biochemistry

authors： Frick K,Schulte M,Friedrich T

更新日期：2015-05-12 00:00:00

abstract：:The nature of the active site cofactor and the amino acid sequence flanking this structure have been determined in a range of copper amine oxidases. For enzymes from porcine plasma, porcine kidney, and pea seedlings, proteolytic digestion was performed on phenylhydrazone or p-nitrophenylhydrazone derivatives. Thermoly...

journal_title：Biochemistry

authors： Janes SM,Palcic MM,Scaman CH,Smith AJ,Brown DE,Dooley DM,Mure M,Klinman JP

更新日期：1992-12-08 00:00:00