Identification of the collagenous proteins synthesized by cultured cells from human skin.

abstract：:Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...

journal_title：Biochemistry

authors： Bazzi MD,Nelsestuen GL

更新日期：1991-08-13 00:00:00

abstract：:The hydrogen-bonded ring NH nuclear magnetic resonance (NMR) spectra of several transfer ribonucleic acid (RNA) species have been examined with particular emphasis on the extreme low-field portion. Betwen --13.8 and --15 ppm there are two extra resonances which are not derived from cloverleaf base pairs. A combined ap...

journal_title：Biochemistry

authors： Hurd RE,Reid BR

更新日期：1979-09-04 00:00:00

abstract：:Napin BnIb is a representative member of the 2S albumin seed proteins, which consists of two polypeptide chains of 3.8 and 8.4 kDa linked by two disulfide bridges. In this work, a complete assignment of the 1H spectra of napin BnIb has been carried out by two-dimensional NMR sequence-specific methods and its secondary...

journal_title：Biochemistry

authors： Rico M,Bruix M,González C,Monsalve RI,Rodríguez R

更新日期：1996-12-10 00:00:00

abstract：:Trimethylamine dehydrogenase (TMADH) from the bacterium Methylophilus methylotrophus (sp. W(3)A(1)) and its C30A mutant were inactivated with three known inactivators of monoamine oxidase, namely, phenylhydrazine, N-cyclopropyl-alpha-methylbenzylamine, and 1-phenylcyclopropylamine. All three compounds irreversibly ina...

journal_title：Biochemistry

authors： Mitchell DJ,Nikolic D,Jang MH,van Breemen RB,Hille R,Silverman RB

更新日期：2001-07-24 00:00:00

abstract：:The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...

journal_title：Biochemistry

authors： Hoyle NP,O'Neill JS

更新日期：2015-01-20 00:00:00

abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

journal_title：Biochemistry

authors： Jung KH,Spudich EN,Dag P,Spudich JL

更新日期：1999-10-05 00:00:00

abstract：:A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by...

journal_title：Biochemistry

authors： Mendelson E,Bustin M

更新日期：1984-07-17 00:00:00

abstract：:Adenosine cyclic 3',5'-phosphate receptor protein (CRP or CAP) is a regulatory protein involved in the transcription of several operons in Escherichia coli. cAMP-independent, nonspecific complexes of CRP and DNA were investigated by photochemical cross-linking of the protein to nonspecific DNA, whose thymines are subs...

journal_title：Biochemistry

authors： Katouzian-Safadi M,Blazy B,Cremet JY,Le Caer JP,Rossier J,Charlier M

更新日期：1993-02-23 00:00:00

abstract：:Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry...

journal_title：Biochemistry

authors： Gaffney JP,Valentine AM

更新日期：2009-12-15 00:00:00

abstract：:Fibrinogen (approximately 10(-5) M) labilizes heterologous interactions within the thrombin-modified factor XIII zymogen (i.e., XIII' = a2'b2) so that, in the time frame (ca. 10 min) of normal clotting in plasma (37 degrees C, mu = 0.15, pH 7.5), 1.5 mM Ca2+ is sufficient to cause the release of the noncatalytic b sub...

journal_title：Biochemistry

authors： Credo RB,Curtis CG,Lorand L

更新日期：1981-06-23 00:00:00

abstract：:The interaction between factor Xa and factor Va was investigated both in solution and in the presence of phospholipid vesicles with varying contents of phosphatidylserine. The binding parameters were inferred from the kinetics of prothrombin activation. Factor Xa and factor Va form in solution an equimolar complex wit...

journal_title：Biochemistry

authors： Lindhout T,Govers-Riemslag JW,van de Waart P,Hemker HC,Rosing J

更新日期：1982-10-26 00:00:00

abstract：:Duplication of a single β-strand that forms part of a β-sheet in photoactive yellow protein (PYP) was found to produce two approximately isoenergetic protein conformations, in which either the first or the second copy of the duplicated β-strand participates in the β-sheet. Whereas one conformation (big-loop) is more s...

journal_title：Biochemistry

authors： Kumar A,Nokhrin S,Woloschuk RM,Woolley GA

更新日期：2018-07-17 00:00:00

abstract：:Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

journal_title：Biochemistry

authors： Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

更新日期：1988-01-12 00:00:00

abstract：:In our previous work [Yang, Z. W., & Babitch, J. A. (1988) Biochemistry (preceding paper in this issue)] divalent cations were found to be more effective promoters of astroglial filament formation than were monovalent cations. To determine if one or more divalent cation binding sites were the basis for this difference...

journal_title：Biochemistry

authors： Yang ZW,Kong CF,Babitch JA

更新日期：1988-09-06 00:00:00

abstract：:Deazaflavins have been found to act as potent catalysts in the photoreduction of flavoproteins in the presence of EDTA and other "photosubstrates". In distinction to the catalysis brought about by normal flavins which involves dark reaction of the photoreduced flavin catalyst, the mechanism of the catalysis by deazafl...

journal_title：Biochemistry

authors： Massey V,Hemmerich P

更新日期：1978-01-10 00:00:00

abstract：:Targeting damage to specific sites in the genome represents an attractive approach to manipulating gene function in mammalian cells. To test the applicability of triple-helix formation as a means for achieving precisely timed site-specific damage within a mammalian gene, a triplex-forming oligodeoxyribonucleotide (TFO...

journal_title：Biochemistry

authors： Vasquez KM,Wensel TG,Hogan ME,Wilson JH

更新日期：1996-08-20 00:00:00

abstract：:Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...

journal_title：Biochemistry

authors： Holden JK,Dejam D,Lewis MC,Huang H,Kang S,Jing Q,Xue F,Silverman RB,Poulos TL

更新日期：2015-07-07 00:00:00

abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...

journal_title：Biochemistry

authors： Anholt RR,Rivers AM

更新日期：1990-05-01 00:00:00

abstract：:We have assayed the cross-linking of oligonucleotides containing repeated mitomycin-reactive CpG sites in order to assess the factors that enhance activation of the carbamoyl function at C10, yielding efficient mitomycin cross-linking. Drugs studied include mitomycin C (MC), N-methylmitomycin A (NMA), and the aziridin...

journal_title：Biochemistry

authors： Cera C,Egbertson M,Teng SP,Crothers DM,Danishefsky SJ

更新日期：1989-06-27 00:00:00

abstract：:In the primary quinone (Q(A)) binding site of Rb. sphaeroides reaction centers (RCs), isoleucine M265 is in extensive van der Waals contact with the ubiquinone headgroup. Substitution of threonine or serine for this residue (mutants M265IT and M265IS), but not valine (mutant M265IV), lowers the redox midpoint potentia...

journal_title：Biochemistry

authors： Wells TA,Takahashi E,Wraight CA

更新日期：2003-04-15 00:00:00

abstract：:The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined. There...

journal_title：Biochemistry

authors： Patton JR

更新日期：1994-08-30 00:00:00

abstract：:The nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (MADHox) from Thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of MADHox as do the monovalent cations Cs+, Rb+, and NH4+. Like the mo...

journal_title：Biochemistry

authors： Gorren AC,Moenne-Loccoz P,Backes G,de Vries S,Sanders-Loehr J,Duine JA

更新日期：1995-10-10 00:00:00

abstract：:The intracellular kinetic folding pathway of the human chorionic gonadotropin beta-subunit (hCG-beta) reveals the presence of a disulfide between Cys residues 38-57 that is not detected by X-ray analysis of secreted hCG-beta. This led us to propose that disulfide rearrangement is an essential feature of cystine knot f...

journal_title：Biochemistry

authors： Wilken JA,Bedows E

更新日期：2004-05-04 00:00:00

abstract：:Most A/T specific heterocyclic diamidine derivatives need at least four A/T base pairs for tight binding to the DNA minor groove. Addition of a GC base pair to A/T sequences typically causes a large decrease in binding constant. The ability to target biologically important sequences of DNA could be significantly incre...

journal_title：Biochemistry

authors： Rahimian M,Kumar A,Say M,Bakunov SA,Boykin DW,Tidwell RR,Wilson WD

更新日期：2009-02-24 00:00:00

abstract：:Ionizing radiation induces clustered DNA damage sites which have been shown to challenge the repair mechanism(s) of the cell. Evidence demonstrating that base excision repair is compromised during the repair of an abasic (AP) site present within a clustered damage site is presented. Simple bistranded clustered damage ...

journal_title：Biochemistry

authors： Lomax ME,Cunniffe S,O'Neill P

更新日期：2004-08-31 00:00:00

abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...

journal_title：Biochemistry

authors： Quay SC,Condie CC

更新日期：1983-02-01 00:00:00

abstract：:The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptide bonds...

journal_title：Biochemistry

authors： Otieno S

更新日期：1978-12-12 00:00:00

abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...

journal_title：Biochemistry

authors： Smirnova IN,Kasho VN,Kaback HR

更新日期：2006-12-26 00:00:00

abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...

journal_title：Biochemistry

authors： Schäfer E,Dencher NA,Vonck J,Parcej DN

更新日期：2007-11-06 00:00:00

abstract：:βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...

journal_title：Biochemistry

authors： Srivastava SS,Jamkhindikar AA,Raman R,Jobby MK,Chadalawada S,Sankaranarayanan R,Sharma Y

更新日期：2017-03-07 00:00:00