Orientation of paramagnetic probes attached to gizzard regulatory light chain bound to myosin heads in rabbit skeletal muscle.

abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...

journal_title：Biochemistry

authors： Dahnke T,Shi Z,Yan H,Jiang RT,Tsai MD

更新日期：1992-07-14 00:00:00

abstract：:3-Oxo-delta 5-steroid isomerase (KSI, EC 5.3.3.1) from Pseudomonas testosteroni catalyzes the isomerization of a variety of 3-oxo-delta 5-steroids to their conjugated Delta4-isomers through the formation of an intermediate dienolate ion. It has previously been found in our laboratory that the aromatic ring of Phe-101 ...

journal_title：Biochemistry

authors： Qi L,Pollack RM

更新日期：1998-05-12 00:00:00

abstract：:Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...

journal_title：Biochemistry

authors： Lee TT,Worby C,Bao ZQ,Dixon JE,Colman RF

更新日期：1998-06-09 00:00:00

abstract：:The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty ac...

journal_title：Biochemistry

authors： Linden CD,Blasie JK,Fox CF

更新日期：1977-04-19 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00

abstract：:The discovery of three mutants in the -synuclein (alphaSyn) gene and the identification of alphaSyn as the major component of Lewy body have opened a new field for understanding the pathogenesis of Parkinson's disease (PD). AlphaSyn is a natively unfolded protein with unknown function and unspecified conformational he...

journal_title：Biochemistry

authors： Jiang C,Chang JY

更新日期：2007-01-16 00:00:00

abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

journal_title：Biochemistry

authors： Chow C,Hegde S,Blanchard JS

更新日期：2017-08-08 00:00:00

abstract：:Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...

journal_title：Biochemistry

authors： Brandt G,Carrasco N,Huang Z

更新日期：2006-07-25 00:00:00

abstract：:Protein prenylation involves the attachment of a hydrophobic isoprenoid moiety to the C-terminus of proteins. Several small GTPases, including members of the Ras and Rho subfamilies, require prenylation for their normal and pathological functions. Recent work has suggested that SmgGDS proteins regulate the prenylation...

journal_title：Biochemistry

authors： Jennings BC,Lawton AJ,Rizk Z,Fierke CA

更新日期：2018-07-24 00:00:00

abstract：:The five-membered nitrogen plus heteroatom rings known as azolines or in their oxidized form as azoles are very common in natural products and drugs. The oxidation of thiazoline to thiazole in the cyanobactin class of natural products is one of the several important transformations that are known to alter the biologic...

journal_title：Biochemistry

authors： Gao S,Ge Y,Bent AF,Schwarz-Linek U,Naismith JH

更新日期：2018-10-16 00:00:00

abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...

journal_title：Biochemistry

authors： Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

更新日期：2000-08-01 00:00:00

abstract：:Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...

journal_title：Biochemistry

authors： Patrie KM,Botelho MJ,Franklin K,Chiu IM

更新日期：1999-07-20 00:00:00

abstract：:Fibrinogen is a ligand for leukocyte integrin alpha(M)beta2 (CD11b/CD18, Mac-1) and mediates adhesion and migration of leukocytes during the immune-inflammatory responses. The binding site for alpha(M)beta2 resides in gammaC, a constituent subdomain in the D-domain of fibrinogen. The sequence gamma383-395 (P2-C) in ga...

journal_title：Biochemistry

authors： Lishko VK,Kudryk B,Yakubenko VP,Yee VC,Ugarova TP

更新日期：2002-10-29 00:00:00

abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...

journal_title：Biochemistry

authors： Jacobson GR,Takacs BJ,Rosenbusch JP

更新日期：1976-06-01 00:00:00

abstract：:The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...

journal_title：Biochemistry

authors： Anas M,Sharma R,Dhamodharan V,Pradeepkumar PI,Manhas A,Srivastava K,Ahmed S,Kumar N

更新日期：2017-12-26 00:00:00

abstract：:The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...

journal_title：Biochemistry

authors： Dhawan IK,Shelver D,Thorsteinsson MV,Roberts GP,Johnson MK

更新日期：1999-09-28 00:00:00

abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

journal_title：Biochemistry

authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

更新日期：2008-12-16 00:00:00

abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...

journal_title：Biochemistry

authors： Henne KR,Fisher MB,Iyer KR,Lang DH,Trager WF,Rettie AE

更新日期：2001-07-24 00:00:00

abstract：:The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...

journal_title：Biochemistry

authors： You JS,Wang M,Lee SH

更新日期：2000-10-24 00:00:00

abstract：:The metalloregulatory protein MntR from Bacillus subtilis acts as a transcriptional regulator of manganese homeostasis. MntR is a member of a subfamily of DtxR-related proteins that perform analogous regulatory functions in a variety of pathogenic organisms. Metal ions activate MntR to bind DNA and repress the transcr...

journal_title：Biochemistry

authors： Lieser SA,Davis TC,Helmann JD,Cohen SM

更新日期：2003-11-04 00:00:00

abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

journal_title：Biochemistry

authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

更新日期：1987-03-24 00:00:00

abstract：:kappa-Bungarotoxin (kappa-bgt) is a 66-residue peptide originally purified from snake venom that acts as an antagonist at certain acetylcholine receptors. It is one of four homologous kappa-neurotoxins that are distinguished from the structurally related alpha-neurotoxins by their ability to block the alpha 3-subunit-...

journal_title：Biochemistry

authors： Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

更新日期：1994-11-08 00:00:00

abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...

journal_title：Biochemistry

authors： Barton R,Khakbaz P,Bera I,Klauda JB,Iovine MK,Berger BW

更新日期：2016-09-06 00:00:00

abstract：:Metal cations represent key elements of RNA structure and function. In the Neurospora VS ribozyme, metal cations play diverse roles; they are important for substrate recognition, formation of the active site, and shifting the pKa's of two key nucleobases that contribute to the general acid-base mechanism. Recently, we...

journal_title：Biochemistry

authors： Bonneau E,Legault P

更新日期：2014-01-28 00:00:00

abstract：:Transient phosphorylation at an aspartate residue on the Spo0F protein is a central step in the phosphorelay signal transduction pathway controlling sporulation in Bacilli. The response regulator Spo0F-P is stable to hydrolysis (t1/2 > 24 h at 23 degrees C in the absence of Mg2+), allowing the use of nondenaturing PAG...

journal_title：Biochemistry

authors： Zapf JW,Hoch JA,Whiteley JM

更新日期：1996-03-05 00:00:00

abstract：:Adenosine A(2B) receptors, which play a role in inflammation and cancer, are of considerable interest as novel drug targets. To gain deeper insights into ligand binding and receptor activation, we exchanged amino acids predicted to be close to the binding pocket. The alanine mutants were stably expressed in CHO cells ...

journal_title：Biochemistry

authors： Thimm D,Schiedel AC,Sherbiny FF,Hinz S,Hochheiser K,Bertarelli DC,Maass A,Müller CE

更新日期：2013-01-29 00:00:00

abstract：:6-Methylsalicylic acid synthase, the multifunctional enzyme complex that catalyzes the biosynthesis of the tetraketide 6-methylsalicylic acid, was modified by thiol-specific inhibitors and cross-linking reagents. Treatment with 1,3-dibromopropan-2-one caused rapid enzyme inactivation and formation of cross-linked dime...

journal_title：Biochemistry

authors： Child CJ,Spencer JB,Bhogal P,Shoolingin-Jordan PM

更新日期：1996-09-24 00:00:00

abstract：:The mechanism by which newly synthesized sterols are transported from their site of synthesis, the endoplasmic reticulum (ER), to the sterol-enriched plasma membrane (PM) is not fully understood. Studies in mammalian cells suggest that newly synthesized cholesterol is transported to the PM in Golgi-bypassing vesicles ...

journal_title：Biochemistry

authors： Baumann NA,Sullivan DP,Ohvo-Rekilä H,Simonot C,Pottekat A,Klaassen Z,Beh CT,Menon AK

更新日期：2005-04-19 00:00:00

abstract：:There are four isoforms of centrin in mammals, with variable sequence, tissue expression, and functional properties. We have recently characterized a number of structural, ion, and target binding properties of human centrin isoform HsCen2. This paper reports a similar characterization of HsCen3, overexpressed in Esche...

journal_title：Biochemistry

authors： Cox JA,Tirone F,Durussel I,Firanescu C,Blouquit Y,Duchambon P,Craescu CT

更新日期：2005-01-25 00:00:00

abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...

journal_title：Biochemistry

authors： Nadal RC,Davies P,Brown DR,Viles JH

更新日期：2009-09-29 00:00:00