Catalytic contribution of phenylalanine-101 of 3-oxo-Delta 5-steroid isomerase.

abstract：:Alpha-synuclein (alpha-syn) is the major component of intracellular inclusions in several neurodegenerative diseases, and the conversion of soluble alpha-syn into filamentous aggregates may contribute to disease pathogenesis. Since mechanisms leading to the formation of alpha-syn inclusions are unclear, in vitro model...

journal_title：Biochemistry

authors： Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

更新日期：2003-07-22 00:00:00

abstract：:Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer seg...

journal_title：Biochemistry

authors： Goldberg AF,Moritz OL,Molday RS

更新日期：1995-10-31 00:00:00

abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...

journal_title：Biochemistry

authors： Lai MZ,Vail WJ,Szoka FC

更新日期：1985-03-26 00:00:00

abstract：:Treatment of soybean lipoxygenase isozyme 1 with its substrates, linoleic acid and oxygen, or product, 13(S)-hydroperoxy-9,11(Z,E)-octadecadienoic acid (13-HPOD), leads to the appearance of a purple color. Although the structure of the chromophore has not been determined, we present strong evidence that it is an Fe(3+...

journal_title：Biochemistry

authors： Nelson MJ,Chase DB,Seitz SP

更新日期：1995-05-09 00:00:00

abstract：:The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...

journal_title：Biochemistry

authors： Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

更新日期：2003-04-22 00:00:00

abstract：:The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...

journal_title：Biochemistry

authors： Bures EJ,Hui JO,Young Y,Chow DT,Katta V,Rohde MF,Zeni L,Rosenfeld RD,Stark KL,Haniu M

更新日期：1998-09-01 00:00:00

abstract：:This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR...

journal_title：Biochemistry

authors： Thapar R,Nicholson EM,Rajagopal P,Waygood EB,Scholtz JM,Klevit RE

更新日期：1996-09-03 00:00:00

abstract：:The residue C221 on pyruvate decarboxylase (EC. 4.1.1.1) from Saccharomyces cerevisiae has been shown to be the site where the substrate activation cascade is triggered [Baburina et al. (1994) Biochemistry 33, 5630-5635] and is located on the beta domain [Arjunan et al. (1996) J. Mol. Biol. 256, 590], while the active...

journal_title：Biochemistry

authors： Baburina I,Dikdan G,Guo F,Tous GI,Root B,Jordan F

更新日期：1998-02-03 00:00:00

abstract：:Indolamine N-methyltransferase (INMT) has been purified to an apparent homogeneity from rabbit lung, and some of its catalytic and physicochemical properties have been examined. The enzyme is a monomeric protein with a molecular weight of 31,500 +/- 1000, a molecular Stokes radius of 21.5 A, and a diffusion coefficien...

journal_title：Biochemistry

authors： Irace G,Colonna G,Camardella M,Della Pietra G,Porta R

更新日期：1982-03-16 00:00:00

abstract：:Myosin subfragment 1 (S1) was covalently labeled with a fluorescent dye, N-[7-(dimethylamino)-4-methyl-3-coumarinyl]maleimide (DACM), and then digested by trypsin to cleave S1 heavy chain into fragments. The DACM-labeled and trypsin-treated S1 was complexed with F-actin and treated with a zero-length cross-linker, 1-e...

journal_title：Biochemistry

authors： Sutoh K

更新日期：1982-09-14 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:Lytic polysaccharide monooxygenases (LPMOs) are ubiquitous oxidoreductases, facilitating the degradation of polymeric carbohydrates in biomass. Cellobiose dehydrogenase (CDH) is a biologically relevant electron donor in this process, with the electrons resulting from cellobiose oxidation being shuttled from the CDH de...

journal_title：Biochemistry

authors： Laurent CVFP,Breslmayr E,Tunega D,Ludwig R,Oostenbrink C

更新日期：2019-03-05 00:00:00

abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

journal_title：Biochemistry

authors： Nath A,Atkins WM

更新日期：2008-01-08 00:00:00

abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

journal_title：Biochemistry

authors： Crute JJ,Wahl AF,Bambara RA

更新日期：1986-01-14 00:00:00

abstract：:4-Oxalocrotonate tautomerase (4-OT) and YwhB, a 4-OT homologue found in Bacillus subtilis, exhibit a low level hydratase activity that converts trans-3-haloacrylates to acetaldehyde, presumably through a malonate semialdehyde intermediate. The mechanism for the initial transformation of the 3-haloacrylate to malonate ...

journal_title：Biochemistry

authors： Wang SC,Johnson WH Jr,Czerwinski RM,Whitman CP

更新日期：2004-01-27 00:00:00

abstract：:Suberyldicholine-induced ion translocation in the millisecond time region in acetylcholine receptor rich membrane vesicles prepared from the electric organ of Electrophorus electricus was investigated in eel Ringer's solution, pH 7.0, 1 degree C. A quench-flow technique with a time resolution of 5 ms was used to measu...

journal_title：Biochemistry

authors： Pasquale EB,Takeyasu K,Udgaonkar JB,Cash DJ,Severski MC,Hess GP

更新日期：1983-12-06 00:00:00

abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...

journal_title：Biochemistry

authors： Matsubara T,Iida M,Tsumuraya T,Fujii I,Sato T

更新日期：2008-07-01 00:00:00

abstract：:N-Ethylmaleimide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activity of the microtubule (MT) motors kinesin, Ncd, and dynein. Currently, the mechanism of inhibition is not known for any of these proteins. To investigate the mechanism by which NEM inhibits Ncd, the recombi...

journal_title：Biochemistry

authors： Phelps KK,Walker RA

更新日期：1999-08-17 00:00:00

abstract：:Circular dichroism spectra of the partially folded trapped intermediates were measured in order to aid in the elucidation of the conformational forces which determine a nonrandom, nonsequential pathway of disulfide bond formation upon refolding of bovine pancreatic trypsin inhibitor. Whatever conformation was responsi...

journal_title：Biochemistry

authors： Kosen PA,Creighton TE,Blout ER

更新日期：1983-05-10 00:00:00

abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...

journal_title：Biochemistry

authors： Zambelli B,Musiani F,Savini M,Tucker P,Ciurli S

更新日期：2007-03-20 00:00:00

abstract：:A highly sensitive and stable scanning microcalorimeter is employed in a reinvestigation of the effect of cholesterol on multilamellar suspensions of dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC). Below 20 mol % cholesterol the DPPC mixtures give heat-capacity curves each of which can...

journal_title：Biochemistry

authors： Mabrey S,Mateo PL,Sturtevant JM

更新日期：1978-06-13 00:00:00

abstract：:Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that during initial transcription of the first 8-10 bases of RNA complexes are relatively unstable, leading to the release of short abortive RNA tra...

journal_title：Biochemistry

authors： Vahia AV,Martin CT

更新日期：2011-08-16 00:00:00

abstract：:Resonances of the histidine region of human carbonic anhydrase B have been studied by proton magnetic resonance spectroscopy in the presence of seven sulfonamide inhibitors. Results of difference spectroscopy and observation of the C-2 resonance of an additional titratable histidine in some of these spectra suggest a ...

journal_title：Biochemistry

authors： Pesando JM,Grollman AP

更新日期：1975-02-25 00:00:00

abstract：:To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...

journal_title：Biochemistry

authors： Shelnutt JA,Rousseau DL,Dethmers JK,Margoliash E

更新日期：1981-10-27 00:00:00

abstract：:Previous work has indicated that PEP mutase catalyzes the rearrangement of phosphoenolpyruvate to phosphonopyruvate by a dissociative mechanism. The crystal structure of the mutase with Mg(II) and sulfopyruvate (a phosphonopyruvate analogue) bound showed that the substrate is anchored to the active site by the Mg(II),...

journal_title：Biochemistry

authors： Liu S,Lu Z,Han Y,Jia Y,Howard A,Dunaway-Mariano D,Herzberg O

更新日期：2004-04-20 00:00:00

abstract：:The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-pho...

journal_title：Biochemistry

authors： Eads JC,Beeby M,Scapin G,Yu TW,Floss HG

更新日期：1999-08-03 00:00:00

abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...

journal_title：Biochemistry

authors： Giacometti GM,Da Ros A,Antonini E,Brunori M

更新日期：1975-04-22 00:00:00

abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

journal_title：Biochemistry

authors： Janes N,Rubin E,Taraschi TF

更新日期：1990-09-11 00:00:00

abstract：:Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...

journal_title：Biochemistry

authors： Horie N,Yamaizumi Z,Kuchino Y,Takai K,Goldman E,Miyazawa T,Nishimura S,Yokoyama S

更新日期：1999-01-05 00:00:00

abstract：:In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...

journal_title：Biochemistry

authors： Murakami N,Kotula L,Hwang YW

更新日期：2000-09-19 00:00:00