1H NMR dipolar echo decay spectroscopy: a sensitive probe of membrane structure.

abstract：:The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a n...

journal_title：Biochemistry

authors： Scoville CD,Abraham GN,Turner DH

更新日期：1979-06-12 00:00:00

abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

journal_title：Biochemistry

authors： Hwang SR,Stoka V,Turk V,Hook VY

更新日期：2005-05-31 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...

journal_title：Biochemistry

authors： Pan LL,Yang Y,Merz KM Jr

更新日期：2014-09-30 00:00:00

abstract：:We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

journal_title：Biochemistry

authors： Sineva EV,Davydov DR

更新日期：2010-12-21 00:00:00

abstract：:Kynureninase is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to give l-alanine and anthranilic acid. beta-Benzoyl-l-alanine, the analogue of l-kynurenine lacking the aromatic amino group, was shown to a good substrate for kynureninase from Pseudomonas fluorescens, an...

journal_title：Biochemistry

authors： Kumar S,Gawandi VB,Capito N,Phillips RS

更新日期：2010-09-14 00:00:00

abstract：:A rapid, three-step purification of DNA alpha-polymerase from calf thymus is described. The key feature is immunoaffinity chromatography using a column of immobilized monoclonal immunoglobulin G (IgG) developed against human KB cell alpha-polymerase. This step is followed by preparative sucrose gradient sedimentation....

journal_title：Biochemistry

authors： Wahl AF,Kowalski SP,Harwell LW,Lord EM,Bambara RA

更新日期：1984-04-24 00:00:00

abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...

journal_title：Biochemistry

authors： Fried MG,Kanugula S,Bromberg JL,Pegg AE

更新日期：1996-12-03 00:00:00

abstract：:Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...

journal_title：Biochemistry

authors： Chu LK,Huang WY,Littledike ET,Hamilton JW,Cohn DV

更新日期：1975-08-12 00:00:00

abstract：:The solvent accessibility of thrombin in its substrate-free and substrate-bound forms has been compared by amide hydrogen/deuterium (H/(2)H) exchange. The optimized inhibitor peptide dPhe-Pro-Arg chloromethyl ketone (PPACK) was used to simulate the substrate-bound form of thrombin. These studies were motivated by the ...

journal_title：Biochemistry

authors： Croy CH,Koeppe JR,Bergqvist S,Komives EA

更新日期：2004-05-11 00:00:00

abstract：:Mass spectrometry was used to identify the sites of covalent attachment of [(14)C]-17alpha-bromoacetamidopropylestradiol ([(14)C]17BAPE(2), an estradiol agonist) to the ligand-binding domain (LBD) of mouse estrogen receptor alpha (ERalpha). A glutathione S-transferase (GST)-LBD chimera protein was overexpressed in Esc...

journal_title：Biochemistry

authors： Mattras H,Aliau S,Richard E,Bonnafous JC,Jouin P,Borgna JL

更新日期：2002-12-31 00:00:00

abstract：:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1992-12-29 00:00:00

abstract：:An empirical preduction algorithm which uses information on the shrs on either side) interactions in 20 proteins to assign every residue in a protein to one of five conformational states is described. The conformational states are defined in terms of the backbone dihedral angles of the residue so that the prediction a...

journal_title：Biochemistry

authors： Maxfield FR,Scheraga HA

更新日期：1976-11-16 00:00:00

abstract：:Simple procedures for the anaerobic preparation of photoactive and stable P840 reaction centers from Chlorobium tepidum and Chlorobium limicola in good yield are presented and quantitated. The subunit composition was tested by cosedimentation in sucrose density gradients. For C. limicola, it minimally comprises four s...

journal_title：Biochemistry

authors： Hager-Braun C,Xie DL,Jarosch U,Herold E,Büttner M,Zimmermann R,Deutzmann R,Hauska G,Nelson N

更新日期：1995-07-25 00:00:00

abstract：:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...

journal_title：Biochemistry

authors： Nishii I,Kataoka M,Tokunaga F,Goto Y

更新日期：1994-04-26 00:00:00

abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

journal_title：Biochemistry

authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

更新日期：2012-08-28 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00

abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

journal_title：Biochemistry

authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

更新日期：2019-02-12 00:00:00

abstract：:Purification and potential tachykinin and enkephalin precursor cleaving enzymes from bovine chromaffin granules was undertaken using as substrates the model precursors 35S-(Met)-beta-preprotachykinin [35S-(Met)-beta-PPT] and 35S-(Met)-preproenkephalin [35S-(Met)-PPE]. Purification by concanavalin A-Sepharose, Sephacry...

journal_title：Biochemistry

authors： Krieger TJ,Hook VY

更新日期：1992-05-05 00:00:00

abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

journal_title：Biochemistry

authors： Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

更新日期：1999-03-30 00:00:00

abstract：:The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinet...

journal_title：Biochemistry

authors： Vandemeulebroucke A,Aldag C,Stiebritz MT,Reiher M,Hilvert D

更新日期：2015-11-10 00:00:00

abstract：:Calcium is required for the substrate binding and for the chemical step of the interfacial catalytic turnover cycle of pancreatic phospholipase A2 (PLA2), but not for the binding of the enzyme to the interface. The role of calcium and other divalent cations (C) is analyzed for the effect on the substrate binding and k...

journal_title：Biochemistry

authors： Yu BZ,Rogers J,Nicol GR,Theopold KH,Seshadri K,Vishweshwara S,Jain MK

更新日期：1998-09-08 00:00:00

abstract：:S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...

journal_title：Biochemistry

authors： Katusz RM,Colman RF

更新日期：1991-11-26 00:00:00

abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...

journal_title：Biochemistry

authors： Furter R,Snaith M,Gillespie DE,Hall BD

更新日期：1992-11-10 00:00:00

abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...

journal_title：Biochemistry

authors： Wolf-Watz M,Grundström T,Härd T

更新日期：2001-09-25 00:00:00

abstract：:The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...

journal_title：Biochemistry

authors： Williams DL Jr,Greene LE

更新日期：1983-05-24 00:00:00

abstract：:CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin's ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic ...

journal_title：Biochemistry

authors： Park D,Pandey SK,Maksimova E,Kole S,Bernier M

更新日期：2000-10-17 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

journal_title：Biochemistry

authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

更新日期：2019-11-26 00:00:00

abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...

journal_title：Biochemistry

authors： Yanchunas J Jr,Dabrowski MJ,Schurke P,Atkins WM

更新日期：1994-12-20 00:00:00