Origin of product selectivity in a prenyl transfer reaction from the same intermediate: exploration of multiple FtmPT1-catalyzed prenyl transfer pathways.

abstract：:ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with compon...

journal_title：Biochemistry

authors： Pérez-Alvarado GC,Martínez-Yamout M,Allen MM,Grosschedl R,Dyson HJ,Wright PE

更新日期：2003-06-24 00:00:00

abstract：:The secondary structure of protein kinase C alpha (PKC alpha) has been studied using infrared spectroscopy in the presence of both H(2)O and D(2)O buffers. In the absence of ligands at 20 degrees C, it was shown that beta-sheet is the major component, representing about 44% of the total structure, whereas the alpha-he...

journal_title：Biochemistry

authors： Torrecillas A,Corbalán-García S,Gómez-Fernández JC

更新日期：2004-03-02 00:00:00

abstract：:The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...

journal_title：Biochemistry

authors： Björnerås J,Gräslund A,Mäler L

更新日期：2013-06-18 00:00:00

abstract：:The binding of various forms of vanadate to myosin and myosin subfragment 1 (S-1) was studied by 51V NMR at increasing vanadate concentrations between 0.06 and 1.0 mM. The distribution of the various forms of vanadate in the solution depended on the total concentration of vanadate. At low concentrations, the predomina...

journal_title：Biochemistry

authors： Ringel I,Peyser YM,Muhlrad A

更新日期：1990-09-25 00:00:00

abstract：:The linear noncompetitive inhibition of the pepsin-catalyzed hydrolysis of Ac-Phe-Phe-Gly at pH 2.1 by L-Ac-Phe, L-Ac-Phe-NH2, and L-Ac-Phe-OEt has been claimed to substantiate the ordered release of products specified by the amino-enzyme mechanism for pepsin action. According to this interpretation, the binding of in...

journal_title：Biochemistry

authors： Silver MS,Stoddard M

更新日期：1975-02-11 00:00:00

abstract：:NADH oxidase activity has been detected in luminal membrane derived from the proximal tubules of rat kidney (brush border membranes). The cytochromes associated with these membranes [García, M.L., Benavides, J., Valdivieso, F., Mayor, F., & Giménez-Gallego, G. (1978) Biochem. Biophys. Res. Commun. 82, 738-744] were re...

journal_title：Biochemistry

authors： Giménez-Gallego G,Benavides J,García ML,Valdivieso F

更新日期：1980-10-14 00:00:00

abstract：:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl...

journal_title：Biochemistry

authors： Mancarella DA,Lenard J

更新日期：1981-11-24 00:00:00

abstract：:The biophysical analysis of multidomain proteins often is difficult because of overlapping signals from the individual domains. Previously, the fluorescent unnatural amino acid p-cyanophenylalanine has been used to study the folding of small single-domain proteins. Here we extend its use to a two-domain protein to sel...

journal_title：Biochemistry

authors： Ries LK,Schmid FX,Schmidpeter PA

更新日期：2016-12-13 00:00:00

abstract：:The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...

journal_title：Biochemistry

authors： Harrison MD,Yanagisawa S,Dennison C

更新日期：2005-03-01 00:00:00

abstract：:The contribution of backbone hydrogen bonds in alpha-helices to the overall stability of a protein has been examined experimentally by replacing several backbone amide linkages in alpha-helix 39-50 of T4 lysozyme with ester linkages. T4 lysozyme variants wherein the backbone amide bonds between residues Ser38 and Leu3...

journal_title：Biochemistry

authors： Koh JT,Cornish VW,Schultz PG

更新日期：1997-09-23 00:00:00

abstract：:The kinetics of charge recombination following photoexcitation by a laser pulse have been analyzed in the reaction center-light harvesting complex 1 (RC-LH1) purified from the photosynthetic bacterium Rhodobacter sphaeroides. In RC-LH1 core complexes isolated from photosynthetically grown cells P(+)Q(B)(-) recombines ...

journal_title：Biochemistry

authors： Francia F,Dezi M,Rebecchi A,Mallardi A,Palazzo G,Melandri BA,Venturoli G

更新日期：2004-11-09 00:00:00

abstract：:A model cosolvent, ethanol, has profound and diversified effects on the amyloidogenic self-assembly of insulin, yielding spectroscopically and morphologically distinguishable forms of beta-aggregates. The alcohol reduces hydrodynamic radii of insulin molecules, decreases enthalpic costs associated with aggregation-pro...

journal_title：Biochemistry

authors： Dzwolak W,Grudzielanek S,Smirnovas V,Ravindra R,Nicolini C,Jansen R,Loksztejn A,Porowski S,Winter R

更新日期：2005-06-28 00:00:00

abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...

journal_title：Biochemistry

authors： Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

更新日期：2005-04-12 00:00:00

abstract：:The refined crystallographic structure of the "closed" conformation of chicken mitochondrial aspartate aminotransferase has been used as a template for the construction of models of the two Escherichia coli aminotransferases encoded by the tyrB and aspC genes. The main results are as follows: (1) Only minor changes ar...

journal_title：Biochemistry

authors： Seville M,Vincent MG,Hahn K

更新日期：1988-11-01 00:00:00

abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...

journal_title：Biochemistry

authors： Polacow I,Cabasso L,Li HJ

更新日期：1976-10-19 00:00:00

abstract：:2-Cys peroxiredoxins (Prxs) are a large and diverse family of peroxidases which, in addition to their antioxidant functions, regulate cell signaling pathways, apoptosis, and differentiation. These enzymes are obligate homodimers (alpha(2)), utilizing a unique intermolecular redox-active disulfide center for the reduct...

journal_title：Biochemistry

authors： Wood ZA,Poole LB,Hantgan RR,Karplus PA

更新日期：2002-04-30 00:00:00

abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...

journal_title：Biochemistry

authors： Caplow M,Shanks J

更新日期：1998-09-15 00:00:00

abstract：:Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the red...

journal_title：Biochemistry

authors： Akagawa M,Ishii Y,Ishii T,Shibata T,Yotsu-Yamashita M,Suyama K,Uchida K

更新日期：2006-12-19 00:00:00

abstract：:The Hsp70-class molecular chaperone HscA interacts specifically with a conserved (99)LPPVK(103) motif of the iron-sulfur cluster scaffold protein IscU. We used a cellulose-bound peptide array to perform single-site saturation substitution of peptide residues corresponding to Glu(98)-Ile(104) of IscU to determine posit...

journal_title：Biochemistry

authors： Tapley TL,Cupp-Vickery JR,Vickery LE

更新日期：2006-07-04 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...

journal_title：Biochemistry

authors： Osborne MJ,Breeze AL,Lian LY,Reilly A,James R,Kleanthous C,Moore GR

更新日期：1996-07-23 00:00:00

abstract：:The chirality of sulfoxidation catalyzed by two cytochrome P-450 isozymes purified from phenobarbital-induced rat liver was studied by using 4-tolyl ethyl sulfide as a substrate. Both P-450 isoenzymes, termed PB-1 and PB-4, when reconstituted with purified rat liver NADPH-cytochrome P-450 reductase and cytochrome b5, ...

journal_title：Biochemistry

authors： Waxman DJ,Light DR,Walsh C

更新日期：1982-05-11 00:00:00

abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

journal_title：Biochemistry

authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

更新日期：2000-07-18 00:00:00

abstract：:We constructed and expressed chimeric receptor cDNAs with insulin receptor exon 3 (residues 191-297 of the cysteine-rich region) replaced with either the comparable region of the insulin-like growth factor I receptor (IGF-IR) or the insulin receptor related receptor (IRR). Both chimeric receptors still could bind insu...

journal_title：Biochemistry

authors： Zhang B,Roth RA

更新日期：1991-05-28 00:00:00

abstract：:To probe the mechanism of chaperone substrate selection, we have investigated the kinetics of complex formation and dissociation between the molecular chaperone DnaK and a short peptide (Cro, representing amino acids 1-12 of the cro repressor protein). The Cro protein was N-terminally labeled with the environmentally ...

journal_title：Biochemistry

authors： Farr CD,Galiano FJ,Witt SN

更新日期：1995-11-28 00:00:00

abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...

journal_title：Biochemistry

authors： Makde RD,Mahajan SK,Kumar V

更新日期：2007-02-27 00:00:00

abstract：:Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...

journal_title：Biochemistry

authors： Kobayashi K,Koppenhöfer A,Ferguson SJ,Tagawa S

更新日期：1997-11-04 00:00:00

abstract：:The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...

journal_title：Biochemistry

authors： Switzer ME,Gordon HJ,McKee PA

更新日期：1983-03-15 00:00:00

abstract：:Stopped-flow and laser photolysis methods have been used to investigate the rates of electron-transfer reactions of fully reduced riboflavin and the three oxidation states of Clostridium pasteurianum flavodoxin. Both normal and 7,8-dichloroflavin analogues were studied. Redox reagents included oxygen, ferricyanide, fe...

journal_title：Biochemistry

authors： Jung J,Tollin G

更新日期：1981-09-01 00:00:00

abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

journal_title：Biochemistry

authors： Sidik K,Smerdon MJ

更新日期：1990-08-14 00:00:00