Occurrence of a reduced nicotinamide adenine dinucleotide oxidase activity linked to a cytochrome system in renal brush border membranes.

abstract：:The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...

journal_title：Biochemistry

authors： Dabitz N,Hu NJ,Yusof AM,Tranter N,Winter A,Daley M,Zschörnig O,Brisson A,Hofmann A

更新日期：2005-12-13 00:00:00

abstract：:CopA, a thermophilic membrane ATPase from Archaeoglobus fulgidus, drives the outward movement of Cu(+) or Ag(+) [Mandal et al. (2002) J. Biol. Chem. 277, 7201-7208]. This, as other P(IB)-ATPases, is characterized by a putative metal binding sequence (C(380)PC(382)) in its sixth transmembrane fragment and cytoplasmic m...

journal_title：Biochemistry

authors： Mandal AK,Argüello JM

更新日期：2003-09-23 00:00:00

abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

journal_title：Biochemistry

authors： Dewey TG,Turner DH

更新日期：1980-04-15 00:00:00

abstract：:Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...

journal_title：Biochemistry

authors： Blackburn JM,Sutherland JD,Baldwin JE

更新日期：1995-06-06 00:00:00

abstract：:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting...

journal_title：Biochemistry

authors： Anderson ET,Fletcher L,Severin A,Murphy E,Baker SM,Matsuka YV

更新日期：2004-09-21 00:00:00

abstract：:HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...

journal_title：Biochemistry

authors： Lin Y,Shenoy SS,Harris RB,Colman RW

更新日期：1996-10-01 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...

journal_title：Biochemistry

authors： Schermerhorn KM,Delaney S

更新日期：2013-10-29 00:00:00

abstract：:A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc(3)Cer) by mass spectrometry and (1)H NMR. The natural occurren...

journal_title：Biochemistry

authors： Hikita T,Tadano-Aritomi K,Iida-Tanaka N,Ishizuka I,Hakomori S

更新日期：2005-07-12 00:00:00

abstract：:The nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (MADHox) from Thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of MADHox as do the monovalent cations Cs+, Rb+, and NH4+. Like the mo...

journal_title：Biochemistry

authors： Gorren AC,Moenne-Loccoz P,Backes G,de Vries S,Sanders-Loehr J,Duine JA

更新日期：1995-10-10 00:00:00

abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...

journal_title：Biochemistry

authors： An S,Yang J,So SW,Zeng L,Goetzl EJ

更新日期：1994-12-06 00:00:00

abstract：:Protein farnesyltransferase catalyzes the posttranslational farnesylation of several proteins involved in signal transduction, including Ras, and is a target enzyme for antitumor therapies. Efficient product formation catalyzed by protein farnesyltransferase requires an enzyme-bound zinc cation and high concentrations...

journal_title：Biochemistry

authors： Saderholm MJ,Hightower KE,Fierke CA

更新日期：2000-10-10 00:00:00

abstract：:A highly sensitive and stable scanning microcalorimeter is employed in a reinvestigation of the effect of cholesterol on multilamellar suspensions of dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC). Below 20 mol % cholesterol the DPPC mixtures give heat-capacity curves each of which can...

journal_title：Biochemistry

authors： Mabrey S,Mateo PL,Sturtevant JM

更新日期：1978-06-13 00:00:00

abstract：:Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...

journal_title：Biochemistry

authors： Northrop DB,Cho YK

更新日期：2000-03-07 00:00:00

abstract：:In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...

journal_title：Biochemistry

authors： Murakami N,Kotula L,Hwang YW

更新日期：2000-09-19 00:00:00

abstract：:Bovine hoof keratin was shown to be a substrate for cAMP-dependent protein kinase using [gamma-32P]ATP. Natural-abundance cross-polarization (CP) MAS 13C NMR was used to examine the effect of phosphorylation on keratin structure. When short contact times were used, phosphorylation was shown to increase the number of r...

journal_title：Biochemistry

authors： Yeagle PL,Frye J,Eckert BS

更新日期：1990-02-13 00:00:00

abstract：:Organisms rely on regulation at the molecular level, such as the allosteric regulation of hemoglobin (Hb) function by anions, to meet challenges presented by changing environmental and physiological conditions. A comparison of the effects of anions on oxygenation, oxidation, and sulfhydryl reactivity of Hb leads us to...

journal_title：Biochemistry

authors： Bonaventura C,Tesh S,Faulkner KM,Kraiter D,Crumbliss AL

更新日期：1998-01-13 00:00:00

abstract：:The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...

journal_title：Biochemistry

authors： Cheshnovsky D,Navon G

更新日期：1980-04-29 00:00:00

abstract：:Cerebrovascular deposition of fibrillar 39-42 amino acid amyloid beta-protein (Abeta), a condition known as cerebral amyloid angiopathy (CAA), is a key pathological feature of Alzheimer's disease and related disorders including hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Severe cases of CAA, ...

journal_title：Biochemistry

authors： Wagner MR,Keane DM,Melchor JP,Auspaker KR,Van Nostrand WE

更新日期：2000-06-27 00:00:00

abstract：:Vesicular stomatitis virus buds from domains of the plasma membrane that have a unique protein and lipid composition. Fluorescence digital imaging microscopy and resonance energy transfer were used to determine how the two viral envelope-associated proteins, the G and the M proteins, could alter the lateral distributi...

journal_title：Biochemistry

authors： Luan P,Yang L,Glaser M

更新日期：1995-08-08 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

journal_title：Biochemistry

authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

更新日期：2010-01-12 00:00:00

abstract：:RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...

journal_title：Biochemistry

authors： Malik SS,Rich E,Viswanathan R,Cairns BR,Fischer CJ

更新日期：2011-09-20 00:00:00

abstract：:Copper binding to the human copper chaperone for superoxide dismutase (hCCS) has been investigated by X-ray absorption spectroscopy. Stoichiometry measurements on the dialyzed, as-isolated protein indicated that up to 3.5 Cu ions bound per hCCS molecule. Reduction with either sodium dithionite or dithiothreitol decrea...

journal_title：Biochemistry

authors： Eisses JF,Stasser JP,Ralle M,Kaplan JH,Blackburn NJ

更新日期：2000-06-27 00:00:00

abstract：:Aromatic amine dehydrogenase (AADH) is the second enzyme known to possess the tryptophan tryptophylquinone (TTQ) prosthetic group. Its ability to catalyze the oxidative deamination of a wide range of aromatic and aliphatic amines has been investigated. Steady-state and transient kinetic studies of the reaction of AADH...

journal_title：Biochemistry

authors： Hyun YL,Davidson VL

更新日期：1995-01-24 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP co...

journal_title：Biochemistry

authors： Stehle R,Lionne C,Travers F,Barman T

更新日期：2000-06-27 00:00:00

abstract：:Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...

journal_title：Biochemistry

authors： Tian F,Lee KC,Hu W,Cross TA

更新日期：1996-09-17 00:00:00

abstract：:NMR studies of the adenosine analog tubercidin have been carried out in the presence of Escherichia coli purine nucleoside phosphorylase (PNP) in order to characterize the conformation of the enzyme-complexed nucleoside. Although analysis of transferred NOE data at various enzyme/inhibitor ratios indicated a predomina...

journal_title：Biochemistry

authors： Perlman ME,Davis DG,Koszalka GW,Tuttle JV,London RE

更新日期：1994-06-21 00:00:00

abstract：:The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted...

journal_title：Biochemistry

authors： Hjörleifsson JG,Ásgeirsson B

更新日期：2017-09-26 00:00:00

abstract：:Oligonucleotides labeled with hexachlorofluorescein (hex) have enabled the interaction of the restriction endonuclease EcoRV with DNA to be evaluated using fluorescence anisotropy. The sensitivity of hex allowed measurements at oligonucleotide concentrations as low as 1 nM, enabling K(D) values in the low nanomolar ra...

journal_title：Biochemistry

authors： Reid SL,Parry D,Liu HH,Connolly BA

更新日期：2001-02-27 00:00:00

abstract：:13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...

journal_title：Biochemistry

authors： Hunter BK,Nicholls KM,Sanders JK

更新日期：1984-01-31 00:00:00