Abstract:
:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl azide (INA) to assess interactions between viral proteins and the lipid envelope. In wild type (wt) virions, the three major structural proteins--G, M, and N--were labeled in the ratio ca. 1.0:0.4:0.2. INA labeled only the membrane-associated peptide of G protein, both in the intact virion and in reconstituted G protein--viral lipid vesicles, demonstrating the specificity of INA for lipid bilayer regions. Labeling of tsM virions with INA resulted in a 2--3-fold greater incorporation into M protein than was found for wt virions, suggesting increased M--membrane associations in the mutant virions. Temperature-stable revertants from tsM possessed wt labeling characteristics. Interaction of the M protein with nucleocapsids was assessed from the abundance of disulfide-linked M--N complexes found after disruption of the virions by sodium dodecyl sulfate solution under nonreducing conditions. The abundance of such complexes was 30--80% less from tsM virions than from wt virions, suggesting decreased M--nucleocapsid interactions in tsM virions. Temperature-stable revertants from tsM resembled wt in the abundance of M--N complex formed. We conclude that the mutations alter M protein in such a way as simultaneously to increase its association with membrane and to decrease its affinity for nucleocapsids in the intact virion.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mancarella DA,Lenard Jdoi
10.1021/bi00527a020subject
Has Abstractpub_date
1981-11-24 00:00:00pages
6872-7issue
24eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Seventeen ribosomal proteins of Saccharomyces cerevisiae were isolated from small ribosomal subunits and disodium ethylenediaminetetraacetate treated 80S ribosomes by chromatography on a column of carboxymethylcellulose and/or by filtration through Sephacryl S-200. The isolated proteins are YS4, YS7, YS8, YS9, YS10, Y...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00262a005
更新日期:1982-09-14 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00043
更新日期:2020-03-17 00:00:00
abstract::Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0027581
更新日期:2001-03-27 00:00:00
abstract::A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00614
更新日期:2020-09-29 00:00:00
abstract::Cytochrome b5 reductase (cb5r), a member of the ferredoxin:NADP+ reductase family of flavoprotein transhydrogenases, catalyzes the NADH-dependent reduction of cytochrome b5. Within this family, a conserved "GxGxxP" sequence motif has been implicated in binding reduced pyridine nucleotides. However, Glycine 179, a cons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051165t
更新日期:2005-10-18 00:00:00
abstract::The reduction of CBrCl3 by the heme-heme oxygenase complex forms dissociable and covalently bound heme products. No such products are formed with mesoheme in which the heme vinyl substituents are replaced by ethyl groups. The dissociable heme products are chromatographically similar but not identical to those obtained...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972720x
更新日期:1998-03-03 00:00:00
abstract::The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00590a031
更新日期:1979-11-13 00:00:00
abstract::We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990473m
更新日期:1999-08-03 00:00:00
abstract::Oncomodulin is a 108-residue, oncodevelopmental protein containing two calcium-binding sites identified as the CD- and EF-loops. The protein contains no tryptophan and only two tyrosine residues, one which is a calcium ligand in the CD-loop (Tyr-57) and one which lies in the flanking D-helix of this loop (Tyr-65). Sit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00244a038
更新日期:1991-07-30 00:00:00
abstract::Lysyl-tRNA synthetase is a member of the class II aminoacyl-tRNA synthetases and catalyses the specific aminoacylation of tRNA(Lys). The crystal structure of the constitutive lysyl-tRNA synthetase (LysS) from Escherichia coli has been determined to 2.7 A resolution in the unliganded form and in a complex with the lysi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001487r
更新日期:2000-10-24 00:00:00
abstract::The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monomers, and related enzyme domains embedded within inteins. DNA-bound structures of homodimeric I-CreI and monomeric I-SceI indicate that three catalytic divalent metal ions are distributed across a pair of overlapping active sites,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048970c
更新日期:2004-11-09 00:00:00
abstract::Rhodobacter sphaeroides strains lacking cytochrome c2 (cyt c2), the normal electron donor to P870+ in light-oxidized reaction center (RC) complexes, are unable to grow photosynthetically. However, spd mutations that suppress the photosynthetic deficiency of cyt c2 mutants elevate levels of the cyt c2 isoform, isocyt c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961648k
更新日期:1997-01-28 00:00:00
abstract::The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002437p
更新日期:2001-05-22 00:00:00
abstract::Spectroscopic and kinetic methods have been used to explore the roles of divalent metal ions in the enolase-catalyzed dehydration of 2-phosphoglycerate (2-PGA). Enolase requires 2 equiv of metal ion per active site for maximal activity. Previous crystallographic studies [Larsen, T. M., Wedekind, J. E., Rayment, I., an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0103922
更新日期:2001-07-10 00:00:00
abstract::Bicyclomycin is a novel, commercially important antibiotic. Information concerning the site of bicyclomycin inhibition in Escherichia coli has been obtained by the production of bicyclomycin resistant mutants by UV irradiation. Selection by growth in the presence of bicyclomycin of a plasmid clone library generated fr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a007
更新日期:1993-04-13 00:00:00
abstract::Cytidine deaminase from Escherichia coli contains 1 mol of tightly bound zinc per enzyme subunit (Yang, C., Carlow, D., Wolfenden, R., & Short, S.A. (1992) Biochemistry 31, 4168-4174). When the metal liganding residues Cys-129 and Cys-132 were replaced by Ala, and His-102 was replaced by Ala, Asn, or Gln, deaminase ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a012
更新日期:1994-05-31 00:00:00
abstract::The concept of using a dynamic base-pairing nucleobase as a mode for degenerate recognition presents a unique challenge to analysis of DNA structure. Proton and phosphorus NMR studies are reported for two nine-residue DNA oligodeoxyribonucleotides, d(CATGGGTAC).d(GTACNCATG) (1) and d(CATGTGTAC).(GTACNCATG) (2), which ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001448f
更新日期:2001-02-13 00:00:00
abstract::Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00314a027
更新日期:1984-09-11 00:00:00
abstract::Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00035a008
更新日期:1995-09-05 00:00:00
abstract::The reaction of ferric cytochrome c peroxidase (CcP) from Saccharomyces cerevisiae with peroxide produces compound I, characterized by both an oxyferryl iron center and a protein-based free radical. The electron paramagnetic resonance (EPR) signal of the CcP compound I radical can be resolved into a broad majority com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a036
更新日期:1991-02-19 00:00:00
abstract::Cytochrome P450 reductase (CPR) is a tethered membrane protein which transfers electrons from NADPH to microsomal P450s. We show that the lipid bilayer has a role in defining the redox potential of the CPR flavin domains. In order to quantitate the electrochemical behavior of this central redox protein, full-length CP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9011435
更新日期:2009-12-29 00:00:00
abstract::The factors that govern the substrate reactivity and stereoselectivity of phosphotriesterase (PTE) toward organophosphotriesters containing various combinations of methyl, ethyl, isopropyl, and phenyl substituents at the phosphorus center were determined by systematic alterations in the dimensions of the active site. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001549d
更新日期:2001-02-06 00:00:00
abstract::Macromolecular protease inhibitors and camelid single-domain antibodies achieve their enzymic inhibition functions often through protruded structures that directly interact with catalytic centers of targeted proteases. Inspired by this phenomenon, we constructed synthetic human antibody libraries encoding long CDR-H3s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00690
更新日期:2020-10-13 00:00:00
abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00560a003
更新日期:1980-09-16 00:00:00
abstract::The interaction of spin-labeled lipids with the myelin proteolipid apoprotein in complexes with dimyristoylphosphatidylcholine of varying lipid/protein ratios has been studied with electron spin resonance spectroscopy. A first shell of approximately 10 lipids per 25 000-dalton protein is found to be motionally restric...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00300a011
更新日期:1984-02-28 00:00:00
abstract::Troponin T (TnT) is an essential element in the thin filament-based regulatory system of striated muscle. Alternative mRNA splicing generates multiple TnT isoforms with primary structural differences in the NH2-terminal region. The functional significance of this hypervariable NH2-terminal domain and the developmental...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9812322
更新日期:1998-10-13 00:00:00
abstract::D-Xylose isomerase (XI) is a heat-stable homotetrameric enzyme used in industry for the production of high-fructose corn syrups by isomerization of D-glucose into D-fructose. To carry out biochemical and structural studies of this enzyme and of its engineered variants, a rapid and convenient method of purification of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00125a009
更新日期:1992-03-17 00:00:00
abstract::In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201248b
更新日期:2011-12-27 00:00:00
abstract::[1-Penicillamine,2-leucine]oxytocin is a conformationally restricted analogue of oxytoxin in which the half-cystine-1 and tyrosine-2 residues of the native hormone are replaced by half-penicillamine (beta, beta-dimethyl-half-cystine) and leucine, respectively. This analogue is a surprisingly potent oxytocin antagonist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00513a018
更新日期:1981-05-12 00:00:00
abstract::By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026848c
更新日期:2003-03-11 00:00:00